LOCUS BBa_K1036005 2880 bp DNA linear 22-Nov-2024 DEFINITION sfgfp (with LVA-tag) under plux R control ACCESSION BBa_K1036005 VERSION BBa_K1036005.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..732 /label=sfGFP misc_feature 733..765 /label=LVA misc_feature 766..771 /label=double stop codon misc_feature 19..771 /label=BBa_K1036004 stem_loop 791..834 /label=stem_loop misc_feature 780..859 /label=BBa_B0010 terminator 780..859 /label=BBa_B0010 stem_loop 875..894 /label=T7 TE misc_feature 901..901 /label=stop polyA_site 895..908 /label=polya misc_feature 868..908 /label=BBa_B0012 terminator 868..908 /label=BBa_B0012 misc_feature 917..936 /label=LuxR/HSL promoter 1031..1038 /label=-10 promoter 1005..1010 /label=-35 misc_feature 1044..1044 /label=start misc_feature 1046..1065 /label=Putative LuxR/HSL misc_feature 917..1067 /label=BBa_R0063 promoter 917..1067 /label=BBa_R0063 misc_feature 1080..1083 /label=conserved RBS 1076..1087 /label=BBa_B0034 misc_feature 1094..1095 /label=prefix misc_feature 1267..1267 /label=T misc_feature 1585..1585 /label=A CDS 1094..1843 /label=luxR misc_feature 1094..1849 /label=BBa_C0062 misc_feature 1850..1874 /label=Help:Barcodes CDS 1094..1849 /label=BBa_C0062 stem_loop 1894..1937 /label=stem_loop misc_feature 1883..1962 /label=BBa_B0010 terminator 1883..1962 /label=BBa_B0010 stem_loop 1978..1997 /label=T7 TE misc_feature 2004..2004 /label=stop polyA_site 1998..2011 /label=polya misc_feature 1971..2011 /label=BBa_B0012 terminator 1971..2011 /label=BBa_B0012 misc_feature 2020..2039 /label=LuxR/HSL promoter 2039..2044 /label=-35 promoter 2061..2066 /label=-10 misc_feature 2072..2072 /label=start misc_feature 2020..2074 /label=BBa_R0062 promoter 2020..2074 /label=BBa_R0062 misc_feature 917..2074 /label=BBa_F2621 misc_feature 2087..2090 /label=conserved RBS 2083..2094 /label=BBa_B0034 misc_feature 2680..2711 /label=LVA CDS 2101..2679 /label=luxI misc_feature 2101..2718 /label=BBa_C0061 misc_feature 2719..2743 /label=Help:Barcodes CDS 2101..2718 /label=BBa_C0061 misc_feature 2083..2743 /label=BBa_C0261 stem_loop 2763..2806 /label=stem_loop misc_feature 2752..2831 /label=BBa_B0010 terminator 2752..2831 /label=BBa_B0010 stem_loop 2847..2866 /label=T7 TE misc_feature 2873..2873 /label=stop polyA_site 2867..2880 /label=polya misc_feature 2840..2880 /label=BBa_B0012 terminator 2840..2880 /label=BBa_B0012 terminator 2752..2880 /label=BBa_B0015 misc_feature 2083..2880 /label=BBa_K082014 misc_feature 917..2880 /label=BBa_K546000 ORIGIN 1 aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt 61 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa 121 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg 181 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat 241 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag 301 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt 361 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc 421 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc 481 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc 541 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg 601 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa 661 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat 721 gaactgtaca aagctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc 781 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 841 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 901 cgtttatata ctagagacct gtacgatcct acaggtgctt atgttaagta attgtattcc 961 cagcgataca atagtgtgac aaaaatccaa tttattagaa tcaaatgtca atccattacc 1021 gttttaatga tatataacac gcaaaacttg cgacaaacaa taggtaatac tagagaaaga 1081 ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat 1141 taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt 1201 acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat 1261 ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat 1321 aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat 1381 atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc 1441 aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct 1501 tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat 1561 gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa 1621 taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga 1681 aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt 1741 ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa 1801 agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg 1861 ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1921 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1981 tcaccttcgg gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt 2041 tacgcaagaa aatggtttgt tatagtcgaa taaatactag agaaagagga gaaatactag 2101 atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga gtataaaggt 2161 attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt agttgtagaa 2221 aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta tgcttgtgat 2281 gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga ttatatgctg 2341 aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc taatatagtc 2401 gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc tgctagtgaa 2461 attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg tattacagaa 2521 tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa agttccttgt 2581 catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt tgtattgtct 2641 atgcctatta atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga cgaaaactac 2701 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc 2761 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2821 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //