##gff-version 3
##sequence-region BBa_K1036005 1 2880
BBa_K1036005	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1036005	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036005	.	terminator	868	908	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1036005	.	engineered_region	868	908	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036005	.	stem_loop	875	894	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036005	.	polyA_site	895	908	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036005	.	stop_codon	901	901	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036005	.	engineered_region	917	2880	.	+	0	ID=BBa_K546000;Name=BBa_K546000
BBa_K1036005	.	engineered_region	2083	2880	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K546000
BBa_K1036005	.	terminator	2752	2880	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K1036005	.	terminator	2840	2880	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1036005	.	engineered_region	2840	2880	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036005	.	stem_loop	2847	2866	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036005	.	polyA_site	2867	2880	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036005	.	stop_codon	2873	2873	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036005	.	terminator	2752	2831	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1036005	.	engineered_region	2752	2831	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036005	.	stem_loop	2763	2806	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036005	.	engineered_region	2083	2743	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K1036005	.	ribosome_entry_site	2083	2094	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1036005	.	conserved_region	2087	2090	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036005	.	CDS	2101	2718	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1036005	.	CDS	2101	2679	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1036005	.	tag	2680	2711	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1036005	.	engineered_tag	2719	2743	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1036005	.	engineered_region	2101	2718	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1036005	.	engineered_region	917	2074	.	+	0	ID=BBa_F2621;Name=BBa_F2621;Parent=BBa_K546000
BBa_K1036005	.	ribosome_entry_site	1076	1087	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1036005	.	conserved_region	1080	1083	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036005	.	promoter	917	1067	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1036005	.	engineered_region	917	1067	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1036005	.	start_codon	1044	1044	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1036005	.	promoter	1031	1038	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1036005	.	non_covalent_binding_site	917	936	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1036005	.	non_covalent_binding_site	1046	1065	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1036005	.	promoter	1005	1010	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1036005	.	terminator	1971	2011	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1036005	.	engineered_region	1971	2011	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036005	.	stem_loop	1978	1997	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036005	.	polyA_site	1998	2011	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036005	.	stop_codon	2004	2004	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036005	.	CDS	1094	1849	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1036005	.	engineered_region	1094	1095	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1036005	.	engineered_region	1094	1849	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1036005	.	engineered_tag	1850	1874	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1036005	.	point_mutation	1585	1585	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1036005	.	point_mutation	1267	1267	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1036005	.	CDS	1094	1843	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1036005	.	terminator	1883	1962	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1036005	.	engineered_region	1883	1962	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036005	.	stem_loop	1894	1937	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036005	.	promoter	2020	2074	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1036005	.	non_covalent_binding_site	2020	2039	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1036005	.	promoter	2039	2044	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1036005	.	engineered_region	2020	2074	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1036005	.	promoter	2061	2066	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1036005	.	start_codon	2072	2072	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1036005	.	terminator	780	859	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1036005	.	engineered_region	780	859	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036005	.	stem_loop	791	834	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036005	.	engineered_region	19	771	.	+	0	ID=BBa_K1036004;Name=BBa_K1036004
BBa_K1036005	.	stop_codon	766	771	.	+	0	ID=annotation2336906;Name=double stop codon;Parent=BBa_K1036004
BBa_K1036005	.	CDS	19	732	.	+	0	ID=annotation2336904;Name=sfGFP;Parent=BBa_K1036004
BBa_K1036005	.	tag	733	765	.	+	0	ID=annotation2336905;Name=LVA;Parent=BBa_K1036004
>BBa_K1036005
aaagaggagaaatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtg
atgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcat
ctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgt
tatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcct
ttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaa
aggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatc
accgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctgg
ctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaag
cgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcat
ggtatggatgaactgtacaaagctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaag
taattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaa
tgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaacataa
atgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctga
tatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttca
atcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagatt
attctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaat
taaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgctt
agttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttc
cttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaaga
atgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcact
ttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggag
caattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatg
gtttgttatagtcgaataaatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttg
gcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttag
ttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaa
tgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaa
cagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataact
ctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaac
agtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcat
gtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaa
acgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata