LOCUS BBa_K1036006 4046 bp DNA linear 22-Dec-2024 DEFINITION lux pL controlled luxR with lux pR controlled sfgfp (with LVA-tag) ACCESSION BBa_K1036006 VERSION BBa_K1036006.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 2083..2102 /label=LuxR/HSL promoter 2197..2204 /label=-10 promoter 2171..2176 /label=-35 misc_feature 2210..2210 /label=start misc_feature 2212..2231 /label=Putative LuxR/HSL misc_feature 2083..2233 /label=BBa_R0063 promoter 2083..2233 /label=BBa_R0063 misc_feature 2246..2249 /label=conserved RBS 2242..2253 /label=BBa_B0034 misc_feature 2260..2261 /label=prefix misc_feature 2433..2433 /label=T misc_feature 2751..2751 /label=A CDS 2260..3009 /label=luxR misc_feature 2260..3015 /label=BBa_C0062 misc_feature 3016..3040 /label=Help:Barcodes CDS 2260..3015 /label=BBa_C0062 stem_loop 3060..3103 /label=stem_loop misc_feature 3049..3128 /label=BBa_B0010 terminator 3049..3128 /label=BBa_B0010 stem_loop 3144..3163 /label=T7 TE misc_feature 3170..3170 /label=stop polyA_site 3164..3177 /label=polya misc_feature 3137..3177 /label=BBa_B0012 terminator 3137..3177 /label=BBa_B0012 misc_feature 3186..3205 /label=LuxR/HSL promoter 3205..3210 /label=-35 promoter 3227..3232 /label=-10 misc_feature 3238..3238 /label=start misc_feature 3186..3240 /label=BBa_R0062 promoter 3186..3240 /label=BBa_R0062 misc_feature 2083..3240 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 CDS 1185..1898 /label=sfGFP misc_feature 1899..1931 /label=LVA misc_feature 1932..1937 /label=double stop codon misc_feature 1185..1937 /label=BBa_K1036004 stem_loop 1957..2000 /label=stem_loop misc_feature 1946..2025 /label=BBa_B0010 terminator 1946..2025 /label=BBa_B0010 stem_loop 2041..2060 /label=T7 TE misc_feature 2067..2067 /label=stop polyA_site 2061..2074 /label=polya misc_feature 2034..2074 /label=BBa_B0012 terminator 2034..2074 /label=BBa_B0012 misc_feature 2083..2102 /label=LuxR/HSL promoter 2197..2204 /label=-10 promoter 2171..2176 /label=-35 misc_feature 2210..2210 /label=start misc_feature 2212..2231 /label=Putative LuxR/HSL misc_feature 2083..2233 /label=BBa_R0063 promoter 2083..2233 /label=BBa_R0063 misc_feature 2246..2249 /label=conserved RBS 2242..2253 /label=BBa_B0034 misc_feature 2260..2261 /label=prefix misc_feature 2433..2433 /label=T misc_feature 2751..2751 /label=A CDS 2260..3009 /label=luxR misc_feature 2260..3015 /label=BBa_C0062 misc_feature 3016..3040 /label=Help:Barcodes CDS 2260..3015 /label=BBa_C0062 stem_loop 3060..3103 /label=stem_loop misc_feature 3049..3128 /label=BBa_B0010 terminator 3049..3128 /label=BBa_B0010 stem_loop 3144..3163 /label=T7 TE misc_feature 3170..3170 /label=stop polyA_site 3164..3177 /label=polya misc_feature 3137..3177 /label=BBa_B0012 terminator 3137..3177 /label=BBa_B0012 misc_feature 3186..3205 /label=LuxR/HSL promoter 3205..3210 /label=-35 promoter 3227..3232 /label=-10 misc_feature 3238..3238 /label=start misc_feature 3186..3240 /label=BBa_R0062 promoter 3186..3240 /label=BBa_R0062 misc_feature 2083..3240 /label=BBa_F2621 misc_feature 3253..3256 /label=conserved RBS 3249..3260 /label=BBa_B0034 misc_feature 3846..3877 /label=LVA CDS 3267..3845 /label=luxI misc_feature 3267..3884 /label=BBa_C0061 misc_feature 3885..3909 /label=Help:Barcodes CDS 3267..3884 /label=BBa_C0061 misc_feature 3249..3909 /label=BBa_C0261 stem_loop 3929..3972 /label=stem_loop misc_feature 3918..3997 /label=BBa_B0010 terminator 3918..3997 /label=BBa_B0010 stem_loop 4013..4032 /label=T7 TE misc_feature 4039..4039 /label=stop polyA_site 4033..4046 /label=polya misc_feature 4006..4046 /label=BBa_B0012 terminator 4006..4046 /label=BBa_B0012 terminator 3918..4046 /label=BBa_B0015 misc_feature 3249..4046 /label=BBa_K082014 misc_feature 2083..4046 /label=BBa_K546000 misc_feature 1167..4046 /label=BBa_K1036005 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgcgt aaaggcgaag 1201 agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata 1261 agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt 1321 tcatctgtac tactggtaaa ctgccggtac cttggccgac tctggtaacg acgctgactt 1381 atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt 1441 ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt 1501 acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga 1561 aaggcattga ctttaaagaa gacggcaata tcctgggcca taagctggaa tacaatttta 1621 acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta 1681 aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac cagcaaaaca 1741 ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc acgcaaagcg 1801 ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg 1861 cagcgggcat cacgcatggt atggatgaac tgtacaaagc tgcaaacgac gaaaactacg 1921 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1981 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 2041 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtac gatcctacag 2101 gtgcttatgt taagtaattg tattcccagc gatacaatag tgtgacaaaa atccaattta 2161 ttagaatcaa atgtcaatcc attaccgttt taatgatata taacacgcaa aacttgcgac 2221 aaacaatagg taatactaga gaaagaggag aaatactaga tgaaaaacat aaatgccgac 2281 gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa 2341 tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat 2401 cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg 2461 caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc 2521 aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca 2581 aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat 2641 acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata 2701 gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat 2761 aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa 2821 aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta 2881 ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca 2941 acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac 3001 tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa 3061 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3121 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 3181 tagagacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataaa 3241 tactagagaa agaggagaaa tactagatga ctataatgat aaaaaaatcg gattttttgg 3301 caattccatc ggaggagtat aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa 3361 gacttgagtg ggacttagtt gtagaaaata accttgaatc agatgagtat gataactcaa 3421 atgcagaata tatttatgct tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat 3481 tacctacaac aggtgattat atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga 3541 gtgctcccaa agatcctaat atagtcgaat taagtcgttt tgctgtaggt aaaaatagct 3601 caaagataaa taactctgct agtgaaatta caatgaaact atttgaagct atatataaac 3661 acgctgttag tcaaggtatt acagaatatg taacagtaac atcaacagca atagagcgat 3721 ttttaaagcg tattaaagtt ccttgtcatc gtattggaga caaagaaatt catgtattag 3781 gtgatactaa atcggttgta ttgtctatgc ctattaatga acagtttaaa aaagcagtct 3841 taaatgctgc aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt 3901 gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 3961 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 4021 ttcgggtggg cctttctgcg tttata //