BBa_K1039001
1
BS1
Bxb1 Invertible Promoter Switch (PB State)
2013-07-07T11:00:00Z
2015-05-08T01:08:50Z
Ghosh, P., Kim, A. I. & Hatfull, G. F. (2003). The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Mol Cell 12, 1101-1111.
The strong constitutive promoter J23119 complementary sequence is flanked by the Bxb1 attP and the complementary sequence of the Bxb1 attB site. In the default state the promoter directs transcription on the negative sense strand towards the attP site. In the presence of the Bxb1 integrase the attP and attB sites are recombined and the promoter is directing transcription to the sense strand towards the attB site.
false
false
_1346_
0
14183
9
In stock
false
The transcriptional terminator J61048 was included to ensure transcription only proceeds in the expected direction. Spacer regions were added adopted from ...
false
Aaron Bender
BBa_K1039001_sequence
1
gctagctcgtggtttgtctggtcaaccaccgcggtctcagtggtgtacggtacaaacccaatagcgaggaggtaaattgtaagctttagggaaacgacatttactgacgcgactttctcaaagctagggtgttggcctgaactgtacgcttggagttgtaggacagacataaaagtatcagatacgcacactaaccatctgctagcattatacctaggactgagctagctgtcaagtgacaactgtataggcgcgagagcgacggcgtgcttccggaccgggccttagcgagcgttagcacggtagtagacctttggggttgaataaccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaaatctattgtactaatcggcttcaacgtgccgcacgggtggcacctcaggaggggcccggatgatcctgacgacggagaccgccgtcgtcgacaagccggccga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z