BBa_K1039006 1 BBa_K1039006 Bxb1 Recombination Directionality Factor 2013-09-09T11:00:00Z 2015-05-08T01:08:50Z The sequence was designed by Bonnet lab at the Department of Bioengineering, Stanford University, and was further modified by Waterloo team. This is the recombination directionality factor (RDF) that acts with Bxb1 serine integrase. It reverses the recombination directionality of Bxb1 integrase (BBa_K1039003). In concert with Bxb1 RDF, Bxb1 integrase (BBa_K1039003) catalyzes site-specific recombination between Bxb1 attR and attL sites, to produce Bxb1 attP and attB sites. This recombination is uni-directional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of Bxb1 integrase and RDF. In concert with Bxb1 RDF (BBa_K1039006), Bxb1 integrase can be used to flip the Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039005 from RL state to PB state. false false _1346_ 0 14649 9 In stock false to be written later. false Urooj Kishor annotation2339258 1 Bxb1 RDF range2339258 1 1 768 BBa_K1039006_sequence 1 atgactcagcgtatcgtctttctacccgatactcagttgcctttcgaggcgcgcaaagagatgcaagcggtcatccgcttcatcggggatgtccagccgtacggcgtggtacatatcggtgacgtcctagacctgccgcagccctcgcgctggaaccggggaaccaagggcgagttcgagggctcggtgtaccgcgacgcggactacgccaagaagaacctgatggagccgctgcgcaaggtctacgacggctggatcgggatgcacgagggcaaccacgatctgcgagcccgcgagtacctggccaagaacgcaccggccctggagggtacgcacgctttcgacatcgacgtgctgctcgacttcgacgggttcggtgtggagctgctgcctgacttctacgacatcgctccgggctggatctccactcacgggcacatgggcaagatgacgctatcccagatcgccggatcgacagcgctcaacggtgccaagaagttcggcaagtccgtggtctgcggccacacgcaccggcaggctgtcgtctcgcactcgttcgggtacggcggctcggtgcgcaagaccgtcaccggcatggaagtcgggcacctgatggacatgaagaaggccaactatctaaagggcggagccgggaactggcagatgggcttcgggatgctcacggtcgacggcaagcatgtcaaggctgagatcgtcccgatcctgggaggcaagttcaccgttgacggccaggtctgggaagtctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z