BBa_J23104 1 BBa_J23104 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters replace later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1039014 1 BBa_K1039014 PhiC31 Intgrase and Recombination Directionality Factor Expression Cassette 2013-09-11T11:00:00Z 2015-05-08T01:08:50Z Biobricks to make this part were synthesized by BioBasic Inc or from the iGEM 2013 distribution. A strong promoter and strong RBS (BBa_K608002) drive expression of PhiC31 integrase (BBa_K1039012) and PhiC31 recombination directionality factor (RDF) (BBa_K1039013). The RBS (BBa_B0034) is associated with RDF. Together, PhiC31 integrase and RDF (BBa_K1039012 and BBa_K1039013) catalyze site-specific recombination between PhiC31 attR and attL sites (Bba_K1039037 and Bba_K1039038). They will also allow flipping of PhiC31 invertible promoter switches BBa_K1039011 and BBa_K1039010 from RL state to PB state. false false _1346_ 0 18339 9 Not in stock false The following mutation was made on PhiC31 integrase Base 51 is changed from T to C, silent mutation. The sequence was synthesized by a third party company. false Jama Hagi-Yusuf component2373584 1 BBa_B0034 component2373582 1 BBa_K1039012 component2373585 1 BBa_K1039013 component2373580 1 BBa_K608002 annotation2373585 1 BBa_K1039013 range2373585 1 2033 2768 annotation2373584 1 BBa_B0034 range2373584 1 2015 2026 annotation2373580 1 BBa_K608002 range2373580 1 1 55 annotation2373582 1 BBa_K1039012 range2373582 1 62 2006 BBa_K1039013 1 BBa_K1039013 PhiC31 Recombination Directionality Factor 2013-09-10T11:00:00Z 2015-05-08T01:08:50Z The sequence was obtained by Genbank and was synthesized by BioBasic Inc This is the recombination directionality factor (RDF) that acts with PhiC31 serine integrase. It reverses the recombination directionality of PhiC31 integrase (BBa_K1039012) In concert with PhiC31 RDF, PhiC31 integrase (BBa_K1039012) catalyzes site-specific recombination between PhiC31 attR and attL sites, to produce PhiC31 attP and attB sites. This recombination is unideirectional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of PhiC31 integrase and RDF. In concert with PhiC31 RDF (BBa_K1039013), PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches (BBa_K1039010 and BBa_K1039011) from RL state to PB state. false false _1346_ 0 18339 9 In stock false Added base after the stop codon to prevent creation of an SpeI site due to addition of suffix false Jama Hagi-Yusuf BBa_K1039012 1 BBa_K1039012 PhiC31 integrase 2013-09-11T11:00:00Z 2015-05-08T01:08:50Z Synthesized DNA from BioBasic. PhiC31 integrase is a serine integrase that catalyzes site-specific recombination between PhiC31 attP (BBa_K103900) and attB (Bba_K1039036) sites to produce PhiC31 attR (Bba_K1039037) and attL (Bba_K1039038) sites. This recombination is unidirectional, i.e. attR and attL do not recombine in the presence of PhiC31 integrase alone. (1) In concert with PhiC31 recombination directionality factor (RDF) (BBa_K1039013), PhiC31 integrase catalyzes site-specific recombination between PhiC31 attR and attL sites, to produce PhiC31 attP and attB sites. This recombination is also unideirectional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of PhiC31 integrase and RDF. PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches BBa_K1039009 and BBa_K1039008 from PB state to RL state. In concert with B PhiC31 RDF (BBa_K1039013), PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches BBa_K1039010 and BBa_K1039011 from RL state to PB state. References: 1. Groth, Amy C., and Michele P. Calos. "Phage Integrases: Biology and Applications." Journal of Molecular Biology. 335.3 (2004): 667-678. false false _1346_ 0 18339 9 In stock false The following mutation was made: Base 51 is changed from T to C, silent mutation. The sequence was synthesized by a third party company. false Jama Hagi-Yusuf annotation2339389 1 PhiC31 integrase range2339389 1 1 1945 BBa_K608002 1 BBa_K608002 strong Promoter and strong RBS 2011-09-14T11:00:00Z 2015-05-08T01:12:52Z assembly from iGEM parts Released HQ 2013 you can insert your part behind this part so it will be immediatly expressed in e.coli. false false _780_ 0 9115 9 In stock false cloning via 3a-assembly false Julia M??ller component2128648 1 BBa_B0034 component2128646 1 BBa_J23104 annotation2128646 1 BBa_J23104 range2128646 1 1 35 annotation2128648 1 BBa_B0034 range2128648 1 44 55 BBa_K1039013_sequence 1 atggcgaagcgttcgatctgggccggagacgaagacaacaagccgaagaagcgtgagacgtacgccgacgacaccgttgggcgtttccactccggctacagcgagacgaatgagcggggcaaggtcgttcccgtcgcgctcgacaagtggcgtatctcgaccggcgaacagtccgttgcggacgctgtggctcagctcttcggcggcaccccggttgagaacgaagagagcacgtctgagaacttcatcgacgtgttcaccgaccggccgaaggtccccgtgatcattgaggctgacggtatccactgggatatgaagctttggctgaacggcaagctgaagcaccactgcgacggctttgacttcgtgtcgcacgccgacgaagagatgatcggtcagccgtgcgggtgcccgaagctcttcgatgagcggaaggcagcggcgaaggagtacgacgcgccgaacccggcaatcaccgtgaccttcacgctcgccgacgacccggagcttggccgcttcaagttccagaccggttcttggacgctcttcaaggttctgcacgaagccgaagacgacgttgagcgcgtcggcaagggtggcgctgtgctcgccaatctcgaacttgagcttgtggagtacacgccgaagcgtggcccgatgcggaacaagcttgtgagctactacaagccgaccatcacggttctgaagtcgtacaacgacgcgattgccgactagg BBa_B0034_sequence 1 aaagaggagaaa BBa_K608002_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa BBa_K1039014_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatggacacgtacgcgggtgcttacgaccgtcagtcgcgcgagcgcgagaactcgagcgcagcaagcccagcgacacagcgtagcgccaacgaagacaaggcggccgaccttcagcgcgaagtcgagcgcgacgggggccggttcaggttcgtcgggcatttcagcgaagcgccgggcacgtcggcgttcgggacggcggagcgcccggagttcgaacgcatcctgaacgaatgccgcgccgggcggctcaacatgatcattgtctatgacgtgtcgcgcttctcgcgcctgaaggtcatggacgcgattccgattgtctcggaattgctcgccctgggcgtgacgattgtttccactcaggaaggcgtcttccggcagggaaacgtcatggacctgattcacctgattatgcggctcgacgcgtcgcacaaagaatcttcgctgaagtcggcgaagattctcgacacgaagaaccttcagcgcgaattgggcgggtacgtcggcgggaaggcgccttacggcttcgagcttgtttcggagacgaaggagatcacgcgcaacggccgaatggtcaatgtcgtcatcaacaagcttgcgcactcgaccactccccttaccggacccttcgagttcgagcccgacgtaatccggtggtggtggcgtgagatcaagacgcacaaacaccttcccttcaagccgggcagtcaagccgccattcacccgggcagcatcacggggctttgtaagcgcatggacgctgacgccgtgccgacccggggcgagacgattgggaagaagaccgcttcaagcgcctgggacccggcaaccgttatgcgaatccttcgggacccgcgtattgcgggcttcgccgctgaggtgatctacaagaagaagccggacggcacgccgaccacgaagattgagggttaccgcattcagcgcgacccgatcacgctccggccggtcgagcttgattgcggaccgatcatcgagcccgctgagtggtatgagcttcaggcgtggttggacggcagggggcgcggcaaggggctttcccgggggcaagccattctgtccgccatggacaagctgtactgcgagtgtggcgccgtcatgacttcgaagcgcggggaagaatcgatcaaggactcttaccgctgccgtcgccggaaggtggtcgacccgtccgcacctgggcagcacgaaggcacgtgcaacgtcagcatggcggcactcgacaagttcgttgcggaacgcatcttcaacaagatcaggcacgccgaaggcgacgaagagacgttggcgcttctgtgggaagccgcccgacgcttcggcaagctcactgaggcgcctgagaagagcggcgaacgggcgaaccttgttgcggagcgcgccgacgccctgaacgcccttgaagagctgtacgaagaccgcgcggcaggcgcgtacgacggacccgttggcaggaagcacttccggaagcaacaggcagcgctgacgctccggcagcaaggggcggaagagcggcttgccgaacttgaagccgccgaagccccgaagcttccccttgaccaatggttccccgaagacgccgacgctgacccgaccggccctaagtcgtggtgggggcgcgcgtcagtagacgacaagcgcgtgttcgtcgggctcttcgtagacaagatcgttgtcacgaagtcgactacgggcagggggcagggaacgcccatcgagaagcgcgcttcgatcacgtgggcgaagccgccgaccgacgacgacgaagacgacgcccaggacggcacggaagacgtagcggcgtagcgagacacccgggaagcctgttaggcgctgagacgggcgcacagcgggcttcctggggcagcgggaagggtcggccggtcccccggtcggcccatttctcttgtctcggtttagttagttagcctaatactagagaaagaggagaaatactagatggcgaagcgttcgatctgggccggagacgaagacaacaagccgaagaagcgtgagacgtacgccgacgacaccgttgggcgtttccactccggctacagcgagacgaatgagcggggcaaggtcgttcccgtcgcgctcgacaagtggcgtatctcgaccggcgaacagtccgttgcggacgctgtggctcagctcttcggcggcaccccggttgagaacgaagagagcacgtctgagaacttcatcgacgtgttcaccgaccggccgaaggtccccgtgatcattgaggctgacggtatccactgggatatgaagctttggctgaacggcaagctgaagcaccactgcgacggctttgacttcgtgtcgcacgccgacgaagagatgatcggtcagccgtgcgggtgcccgaagctcttcgatgagcggaaggcagcggcgaaggagtacgacgcgccgaacccggcaatcaccgtgaccttcacgctcgccgacgacccggagcttggccgcttcaagttccagaccggttcttggacgctcttcaaggttctgcacgaagccgaagacgacgttgagcgcgtcggcaagggtggcgctgtgctcgccaatctcgaacttgagcttgtggagtacacgccgaagcgtggcccgatgcggaacaagcttgtgagctactacaagccgaccatcacggttctgaagtcgtacaacgacgcgattgccgactagg BBa_J23104_sequence 1 ttgacagctagctcagtcctaggtattgtgctagc BBa_K1039012_sequence 1 atggacacgtacgcgggtgcttacgaccgtcagtcgcgcgagcgcgagaactcgagcgcagcaagcccagcgacacagcgtagcgccaacgaagacaaggcggccgaccttcagcgcgaagtcgagcgcgacgggggccggttcaggttcgtcgggcatttcagcgaagcgccgggcacgtcggcgttcgggacggcggagcgcccggagttcgaacgcatcctgaacgaatgccgcgccgggcggctcaacatgatcattgtctatgacgtgtcgcgcttctcgcgcctgaaggtcatggacgcgattccgattgtctcggaattgctcgccctgggcgtgacgattgtttccactcaggaaggcgtcttccggcagggaaacgtcatggacctgattcacctgattatgcggctcgacgcgtcgcacaaagaatcttcgctgaagtcggcgaagattctcgacacgaagaaccttcagcgcgaattgggcgggtacgtcggcgggaaggcgccttacggcttcgagcttgtttcggagacgaaggagatcacgcgcaacggccgaatggtcaatgtcgtcatcaacaagcttgcgcactcgaccactccccttaccggacccttcgagttcgagcccgacgtaatccggtggtggtggcgtgagatcaagacgcacaaacaccttcccttcaagccgggcagtcaagccgccattcacccgggcagcatcacggggctttgtaagcgcatggacgctgacgccgtgccgacccggggcgagacgattgggaagaagaccgcttcaagcgcctgggacccggcaaccgttatgcgaatccttcgggacccgcgtattgcgggcttcgccgctgaggtgatctacaagaagaagccggacggcacgccgaccacgaagattgagggttaccgcattcagcgcgacccgatcacgctccggccggtcgagcttgattgcggaccgatcatcgagcccgctgagtggtatgagcttcaggcgtggttggacggcagggggcgcggcaaggggctttcccgggggcaagccattctgtccgccatggacaagctgtactgcgagtgtggcgccgtcatgacttcgaagcgcggggaagaatcgatcaaggactcttaccgctgccgtcgccggaaggtggtcgacccgtccgcacctgggcagcacgaaggcacgtgcaacgtcagcatggcggcactcgacaagttcgttgcggaacgcatcttcaacaagatcaggcacgccgaaggcgacgaagagacgttggcgcttctgtgggaagccgcccgacgcttcggcaagctcactgaggcgcctgagaagagcggcgaacgggcgaaccttgttgcggagcgcgccgacgccctgaacgcccttgaagagctgtacgaagaccgcgcggcaggcgcgtacgacggacccgttggcaggaagcacttccggaagcaacaggcagcgctgacgctccggcagcaaggggcggaagagcggcttgccgaacttgaagccgccgaagccccgaagcttccccttgaccaatggttccccgaagacgccgacgctgacccgaccggccctaagtcgtggtgggggcgcgcgtcagtagacgacaagcgcgtgttcgtcgggctcttcgtagacaagatcgttgtcacgaagtcgactacgggcagggggcagggaacgcccatcgagaagcgcgcttcgatcacgtgggcgaagccgccgaccgacgacgacgaagacgacgcccaggacggcacggaagacgtagcggcgtagcgagacacccgggaagcctgttaggcgctgagacgggcgcacagcgggcttcctggggcagcgggaagggtcggccggtcccccggtcggcccatttctcttgtctcggtttagttagttagcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z