LOCUS BBa_K1039016 8356 bp DNA linear 22-Nov-2024 DEFINITION HPdO Helper Plasmid ACCESSION BBa_K1039016 VERSION BBa_K1039016.1 FEATURES Location/Qualifiers CDS 1..1233 /label=pII CDS 898..1233 /label=pX CDS 1245..1508 /label=pV CDS 1510..1611 /label=pVII CDS 1608..1706 /label=pIX CDS 1703..1924 /label=pVIII CDS 1981..3255 /label=pIII CDS 3258..3596 /label=pVI CDS 3598..4644 /label=pI CDS 4622..5902 /label=pIV misc_feature 5918..6780 /label=p15A CDS 7229..8044 /label=Kanamycin Resistance Protein ORIGIN 1 atgattgaca tgctagtttt acgattaccg ttcatcgatt ctcttgtttg ctccagactc 61 tcaggcaatg acctgatagc ctttgtagac ctctcaaaaa tagctaccct ctccggcatg 121 aatttatcag ctagaacggt tgaatatcat gttgatggtg atttgactgt ctccggcctt 181 tctcaccctt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag 241 ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag 301 ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat 361 tttgctaatt ctttgccttg cctgtatgat ttattggatg ttaacgctac tactattagt 421 agaattgatg ccaccttttc agctcgcgcc ccaaatgaaa atatagctaa acaggttatt 481 gaccatttgc gaaatgtatc taatggtcaa actaaatcta ctcgttcgca gaattgggaa 541 tcaactgtta catggaatga aacttccaga caccgtactt tagttgcata tttaaaacat 601 gttgagctac agcaccagat tcagcaatta agctctaagc catccgcaaa aatgacctct 661 tatcaaaagg agcaattaaa ggtactctct aatcctgacc tgttggagtt tgcttccggt 721 ctggttcgct ttgaagctcg aattaaaacg cgatatttga agtctttcgg gcttcctctt 781 aatctttttg atgcaatccg ctttgcttct gactataata gtcagggtaa agacctgatt 841 tttgatttat ggtcattctc gttttctgaa ctgtttaaag catttgaggg ggattcaatg 901 aatatttatg acgattccgc agtattggac gctatccagt ctaaacattt tactattacc 961 ccctctggca aaacttcttt tgcaaaagcc tctcgctatt ttggttttta tcgtcgtctg 1021 gtaaacgagg gttatgatag tgttgctctt actatgcctc gtaattcctt ttggcgttat 1081 gtatctgcat tagttgaatg tggtattcct aaatctcaac tgatgaatct ttctacctgt 1141 aataatgttg ttccgttagt tcgttttatt aacgtagatt tttcttccca acgtcctgac 1201 tggtataatg agccagttct taaaatcgca taaggtaatt cacaatgatt aaagttgaaa 1261 ttaaaccatc tcaagcccaa tttactactc gttctggtgt ttctcgtcag ggcaagcctt 1321 attcactgaa tgagcagctt tgttacgttg atttgggtaa tgaatatccg gttcttgtca 1381 agattactct tgatgaaggt cagccagcct atgcgcctgg tctgtacacc gttcatctgt 1441 cctctttcaa agttggtcag ttcggttccc ttatgattga ccgtctgcgc ctcgttccgg 1501 ctaagtaaca tggagcaggt cgcggatttc gacacaattt atcaggcgat gatacaaatc 1561 tccgttgtac tttgtttcgc gcttggtata atcgctgggg gtcaaagatg agtgttttag 1621 tgtattcttt cgcctctttc gttttaggtt ggtgccttcg tagtggcatt acgtatttta 1681 cccgtttaat ggaaacttcc tcatgaaaaa gtctttagtc ctcaaagcct ctgtagccgt 1741 tgctaccctc gttccgatgc tgtctttcgc tgctgagggt gacgatcccg caaaagcggc 1801 ctttaactcc ctgcaagcct cagcgaccga atatatcggt tatgcgtggg cgatggttgt 1861 tgtcattgtc ggcgcaacta tcggtatcaa gctgtttaag aaattcacct cgaaagcaag 1921 ctgataaacc gatacaatta aaggctcctt ttggagcctt tttttttgga gattttcaac 1981 gtgaaaaaat tattattcgc aattccttta gttgttcctt tctattctca ctccgctgaa 2041 actgttgaaa gttgtttagc aaaaccccat acagaaaatt catttactaa cgtctggaaa 2101 gacgacaaaa ctttagatcg ttacgctaac tatgagggct gtctgtggaa tgctacaggc 2161 gttgtagttt gtactggtga cgaaactcag tgttacggta catgggttcc tattgggctt 2221 gctatccctg aaaatgaggg tggtggctct gagggtggcg gttctgaggg tggcggttct 2281 gagggtggcg gtactaaacc tcctgagtac ggtgatacac ctattccggg ctatacttat 2341 atcaaccctc tcgacggcac ttatccgcct ggtactgagc aaaaccccgc taatcctaat 2401 ccttctcttg aggagtctca gcctcttaat actttcatgt ttcagaataa taggttccga 2461 aataggcagg gggcattaac tgtttatacg ggcactgtta ctcaaggcac tgaccccgtt 2521 aaaacttatt accagtacac tcctgtatca tcaaaagcca tgtatgacgc ttactggaac 2581 ggtaaattca gagactgcgc tttccattct ggctttaatg aggatccatt cgtttgtgaa 2641 tatcaaggcc aatcgtctga cctgcctcaa cctcctgtca atgctggcgg cggctctggt 2701 ggtggttctg gtggcggctc tgagggtggt ggctctgagg gtggcggttc tgagggtggc 2761 ggctctgagg gaggcggttc cggtggtggc tctggttccg gtgattttga ttatgaaaag 2821 atggcaaacg ctaataaggg ggctatgacc gaaaatgccg atgaaaacgc gctacagtct 2881 gacgctaaag gcaaacttga ttctgtcgct actgattacg gtgctgctat cgatggtttc 2941 attggtgacg tttccggcct tgctaatggt aatggtgcta ctggtgattt tgctggctct 3001 aattcccaaa tggctcaagt cggtgacggt gataattcac ctttaatgaa taatttccgt 3061 caatatttac cttccctccc tcaatcggtt gaatgtcgcc cttttgtctt tggcgctggt 3121 aaaccatatg aattttctat tgattgtgac aaaataaact tattccgtgg tgtctttgcg 3181 tttcttttat atgttgccac ctttatgtat gtattttcta cgtttgctaa catactgcgt 3241 aataaggagt cttaatcatg ccagttcttt tgggtattcc gttattattg cgtttcctcg 3301 gtttccttct ggtaactttg ttcggctatc tgcttacttt tcttaaaaag ggcttcggta 3361 agatagctat tgctatttca ttgtttcttg ctcttattat tgggcttaac tcaattcttg 3421 tgggttatct ctctgatatt agcgctcaat taccctctga ctttgttcag ggtgttcagt 3481 taattctccc gtctaatgcg cttccctgtt tttatgttat tctctctgta aaggctgcta 3541 ttttcatttt tgacgttaaa caaaaaatcg tttcttattt ggattgggat aaataatatg 3601 gctgtttatt ttgtaactgg caaattaggc tctggaaaga cgctcgttag cgttggtaag 3661 attcaggata aaattgtagc tgggtgcaaa atagcaacta atcttgattt aaggcttcaa 3721 aacctcccgc aagtcgggag gttcgctaaa acgcctcgcg ttcttagaat accggataag 3781 ccttctatat ctgatttgct tgctattggg cgcggtaatg attcctacga tgaaaataaa 3841 aacggcttgc ttgttctcga tgagtgcggt acttggttta atacccgttc ttggaatgat 3901 aaggaaagac agccgattat tgattggttt ctacatgctc gtaaattagg atgggatatt 3961 atttttcttg ttcaggactt atctattgtt gataaacagg cgcgttctgc attagctgaa 4021 catgttgttt attgtcgtcg tctggacaga attactttac cttttgtcgg tactttatat 4081 tctcttatta ctggctcgaa aatgcctctg cctaaattac atgttggcgt tgttaaatat 4141 ggcgattctc aattaagccc tactgttgag cgttggcttt atactggtaa gaatttgtat 4201 aacgcatatg atactaaaca ggctttttct agtaattatg attccggtgt ttattcttat 4261 ttaacgcctt atttatcaca cggtcggtat ttcaaaccat taaatttagg tcagaagatg 4321 aaattaacta aaatatattt gaaaaagttt tctcgcgttc tttgtcttgc gattggattt 4381 gcatcagcat ttacatatag ttatataacc caacctaagc cggaggttaa aaaggtagtc 4441 tctcagacct atgattttga taaattcact attgactctt ctcagcgtct taatctaagc 4501 tatcgctatg ttttcaagga ttctaaggga aaattaatta atagcgacga tttacagaag 4561 caaggttatt cactcacata tattgattta tgtactgttt ccattaaaaa aggtaattca 4621 aatgaaattg ttaaatgtaa ttaattttgt tttcttgatg tttgtttcat catcttcttt 4681 tgctcaggta attgaaatga ataattcgcc tctgcgcgat tttgtaactt ggtattcaaa 4741 gcaatcaggc gaatccgtta ttgtttctcc cgatgtaaaa ggtactgtta ctgtatattc 4801 atctgacgtt aaacctgaaa atctacgcaa tttctttatt tctgttttac gtgcaaataa 4861 ttttgatatg gtaggttcta acccttccat tattcagaag tataatccaa acaatcagga 4921 ttatattgat gaattgccat catctgataa tcaggaatat gatgataatt ccgctccttc 4981 tggtggtttc tttgttccgc aaaatgataa tgttactcaa acttttaaaa ttaataacgt 5041 tcgggcaaag gatttaatac gagttgtcga attgtttgta aagtctaata cttctaaatc 5101 ctcaaatgta ttatctattg acggctctaa tctattagtt gttagtgctc ctaaagatat 5161 tttagataac cttcctcaat tcctttcaac tgttgatttg ccaactgacc agatattgat 5221 tgagggtttg atatttgagg ttcagcaagg tgatgcttta gatttttcat ttgctgctgg 5281 ctctcagcgt ggcactgttg caggcggtgt taatactgac cgcctcacct ctgttttatc 5341 ttctgctggt ggttcgttcg gtatttttaa tggcgatgtt ttagggctat cagttcgcgc 5401 attaaagact aatagccatt caaaaatatt gtctgtgcca cgtattctta cgctttcagg 5461 tcagaagggt tctatctctg ttggccagaa tgtccctttt attactggtc gtgtgactgg 5521 tgaatctgcc aatgtaaata atccatttca gacgattgag cgtcaaaatg taggtatttc 5581 catgagcgtt tttcctgttg caatggctgg cggtaatatt gttctggata ttaccagcaa 5641 ggccgatagt ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat 5701 tgctacaacg gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta 5761 taaaaacact tctcaggatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct 5821 cctgtttagc tcccgctctg attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc 5881 aaccatagta cgcgccctgt agcggcgcat taagcgctag cagtgagctt catgtggcag 5941 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc 6001 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga 6061 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc 6121 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc 6181 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc 6241 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta 6301 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa 6361 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta 6421 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa 6481 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa 6541 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga 6601 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc 6661 gcagaccaaa acgatctcaa gaagatcatc ttattaaggg gtctgacgct cagtggaacg 6721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 6901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7021 taaaccagcc agccgattcg agctcgcccg gggatcgacc agttggtgat tttgaacttt 7081 tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca 7141 gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta atgctctgcc 7201 agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact 7261 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg 7321 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga 7381 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat 7441 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca 7501 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat 7561 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt 7621 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat 7681 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg 7741 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg 7801 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg 7861 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc 7921 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat 7981 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc 8041 tcataacccc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata 8101 tatttttatc ttgtgcaatg taacatcaga gattttgaaa cacaacgtgg ctttcccccc 8161 cccccccctg caggtctcgg gctattcttt tgatttataa gggattttgc cgatttcggc 8221 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 8281 aacgtttaca atttaaatat ttgcttatac aatcttcctg tttttggggc ttttcttatt 8341 atcaaccggg gtacat //