LOCUS BBa_K1039017 8172 bp DNA linear 22-Nov-2024 DEFINITION HPdO with deleted gVIII Helper Plasmid ACCESSION BBa_K1039017 VERSION BBa_K1039017.1 FEATURES Location/Qualifiers CDS 1..1233 /label=pII CDS 898..1233 /label=pX CDS 1245..1508 /label=pV CDS 1510..1611 /label=pVII CDS 1608..1706 /label=pIX CDS 1797..3071 /label=pIII CDS 3074..3412 /label=pVI CDS 3414..4460 /label=pI misc_feature 1707..1712 /label=BglII CDS 4438..5718 /label=pIV misc_feature 5734..6596 /label=p15A CDS 7045..7860 /label=Kanamycin Resistance Protein ORIGIN 1 atgattgaca tgctagtttt acgattaccg ttcatcgatt ctcttgtttg ctccagactc 61 tcaggcaatg acctgatagc ctttgtagac ctctcaaaaa tagctaccct ctccggcatg 121 aatttatcag ctagaacggt tgaatatcat gttgatggtg atttgactgt ctccggcctt 181 tctcaccctt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag 241 ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag 301 ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat 361 tttgctaatt ctttgccttg cctgtatgat ttattggatg ttaacgctac tactattagt 421 agaattgatg ccaccttttc agctcgcgcc ccaaatgaaa atatagctaa acaggttatt 481 gaccatttgc gaaatgtatc taatggtcaa actaaatcta ctcgttcgca gaattgggaa 541 tcaactgtta catggaatga aacttccaga caccgtactt tagttgcata tttaaaacat 601 gttgagctac agcaccagat tcagcaatta agctctaagc catccgcaaa aatgacctct 661 tatcaaaagg agcaattaaa ggtactctct aatcctgacc tgttggagtt tgcttccggt 721 ctggttcgct ttgaagctcg aattaaaacg cgatatttga agtctttcgg gcttcctctt 781 aatctttttg atgcaatccg ctttgcttct gactataata gtcagggtaa agacctgatt 841 tttgatttat ggtcattctc gttttctgaa ctgtttaaag catttgaggg ggattcaatg 901 aatatttatg acgattccgc agtattggac gctatccagt ctaaacattt tactattacc 961 ccctctggca aaacttcttt tgcaaaagcc tctcgctatt ttggttttta tcgtcgtctg 1021 gtaaacgagg gttatgatag tgttgctctt actatgcctc gtaattcctt ttggcgttat 1081 gtatctgcat tagttgaatg tggtattcct aaatctcaac tgatgaatct ttctacctgt 1141 aataatgttg ttccgttagt tcgttttatt aacgtagatt tttcttccca acgtcctgac 1201 tggtataatg agccagttct taaaatcgca taaggtaatt cacaatgatt aaagttgaaa 1261 ttaaaccatc tcaagcccaa tttactactc gttctggtgt ttctcgtcag ggcaagcctt 1321 attcactgaa tgagcagctt tgttacgttg atttgggtaa tgaatatccg gttcttgtca 1381 agattactct tgatgaaggt cagccagcct atgcgcctgg tctgtacacc gttcatctgt 1441 cctctttcaa agttggtcag ttcggttccc ttatgattga ccgtctgcgc ctcgttccgg 1501 ctaagtaaca tggagcaggt cgcggatttc gacacaattt atcaggcgat gatacaaatc 1561 tccgttgtac tttgtttcgc gcttggtata atcgctgggg gtcaaagatg agtgttttag 1621 tgtattcttt cgcctctttc gttttaggtt ggtgccttcg tagtggcatt acgtatttta 1681 cccgtttaat ggaaacttcc tcatgaagat cttaagaaat tcacctcgaa agcaagctga 1741 taaaccgata caattaaagg ctccttttgg agcctttttt tttggagatt ttcaacgtga 1801 aaaaattatt attcgcaatt cctttagttg ttcctttcta ttctcactcc gctgaaactg 1861 ttgaaagttg tttagcaaaa ccccatacag aaaattcatt tactaacgtc tggaaagacg 1921 acaaaacttt agatcgttac gctaactatg agggctgtct gtggaatgct acaggcgttg 1981 tagtttgtac tggtgacgaa actcagtgtt acggtacatg ggttcctatt gggcttgcta 2041 tccctgaaaa tgagggtggt ggctctgagg gtggcggttc tgagggtggc ggttctgagg 2101 gtggcggtac taaacctcct gagtacggtg atacacctat tccgggctat acttatatca 2161 accctctcga cggcacttat ccgcctggta ctgagcaaaa ccccgctaat cctaatcctt 2221 ctcttgagga gtctcagcct cttaatactt tcatgtttca gaataatagg ttccgaaata 2281 ggcagggggc attaactgtt tatacgggca ctgttactca aggcactgac cccgttaaaa 2341 cttattacca gtacactcct gtatcatcaa aagccatgta tgacgcttac tggaacggta 2401 aattcagaga ctgcgctttc cattctggct ttaatgagga tccattcgtt tgtgaatatc 2461 aaggccaatc gtctgacctg cctcaacctc ctgtcaatgc tggcggcggc tctggtggtg 2521 gttctggtgg cggctctgag ggtggtggct ctgagggtgg cggttctgag ggtggcggct 2581 ctgagggagg cggttccggt ggtggctctg gttccggtga ttttgattat gaaaagatgg 2641 caaacgctaa taagggggct atgaccgaaa atgccgatga aaacgcgcta cagtctgacg 2701 ctaaaggcaa acttgattct gtcgctactg attacggtgc tgctatcgat ggtttcattg 2761 gtgacgtttc cggccttgct aatggtaatg gtgctactgg tgattttgct ggctctaatt 2821 cccaaatggc tcaagtcggt gacggtgata attcaccttt aatgaataat ttccgtcaat 2881 atttaccttc cctccctcaa tcggttgaat gtcgcccttt tgtctttggc gctggtaaac 2941 catatgaatt ttctattgat tgtgacaaaa taaacttatt ccgtggtgtc tttgcgtttc 3001 ttttatatgt tgccaccttt atgtatgtat tttctacgtt tgctaacata ctgcgtaata 3061 aggagtctta atcatgccag ttcttttggg tattccgtta ttattgcgtt tcctcggttt 3121 ccttctggta actttgttcg gctatctgct tacttttctt aaaaagggct tcggtaagat 3181 agctattgct atttcattgt ttcttgctct tattattggg cttaactcaa ttcttgtggg 3241 ttatctctct gatattagcg ctcaattacc ctctgacttt gttcagggtg ttcagttaat 3301 tctcccgtct aatgcgcttc cctgttttta tgttattctc tctgtaaagg ctgctatttt 3361 catttttgac gttaaacaaa aaatcgtttc ttatttggat tgggataaat aatatggctg 3421 tttattttgt aactggcaaa ttaggctctg gaaagacgct cgttagcgtt ggtaagattc 3481 aggataaaat tgtagctggg tgcaaaatag caactaatct tgatttaagg cttcaaaacc 3541 tcccgcaagt cgggaggttc gctaaaacgc ctcgcgttct tagaataccg gataagcctt 3601 ctatatctga tttgcttgct attgggcgcg gtaatgattc ctacgatgaa aataaaaacg 3661 gcttgcttgt tctcgatgag tgcggtactt ggtttaatac ccgttcttgg aatgataagg 3721 aaagacagcc gattattgat tggtttctac atgctcgtaa attaggatgg gatattattt 3781 ttcttgttca ggacttatct attgttgata aacaggcgcg ttctgcatta gctgaacatg 3841 ttgtttattg tcgtcgtctg gacagaatta ctttaccttt tgtcggtact ttatattctc 3901 ttattactgg ctcgaaaatg cctctgccta aattacatgt tggcgttgtt aaatatggcg 3961 attctcaatt aagccctact gttgagcgtt ggctttatac tggtaagaat ttgtataacg 4021 catatgatac taaacaggct ttttctagta attatgattc cggtgtttat tcttatttaa 4081 cgccttattt atcacacggt cggtatttca aaccattaaa tttaggtcag aagatgaaat 4141 taactaaaat atatttgaaa aagttttctc gcgttctttg tcttgcgatt ggatttgcat 4201 cagcatttac atatagttat ataacccaac ctaagccgga ggttaaaaag gtagtctctc 4261 agacctatga ttttgataaa ttcactattg actcttctca gcgtcttaat ctaagctatc 4321 gctatgtttt caaggattct aagggaaaat taattaatag cgacgattta cagaagcaag 4381 gttattcact cacatatatt gatttatgta ctgtttccat taaaaaaggt aattcaaatg 4441 aaattgttaa atgtaattaa ttttgttttc ttgatgtttg tttcatcatc ttcttttgct 4501 caggtaattg aaatgaataa ttcgcctctg cgcgattttg taacttggta ttcaaagcaa 4561 tcaggcgaat ccgttattgt ttctcccgat gtaaaaggta ctgttactgt atattcatct 4621 gacgttaaac ctgaaaatct acgcaatttc tttatttctg ttttacgtgc aaataatttt 4681 gatatggtag gttctaaccc ttccattatt cagaagtata atccaaacaa tcaggattat 4741 attgatgaat tgccatcatc tgataatcag gaatatgatg ataattccgc tccttctggt 4801 ggtttctttg ttccgcaaaa tgataatgtt actcaaactt ttaaaattaa taacgttcgg 4861 gcaaaggatt taatacgagt tgtcgaattg tttgtaaagt ctaatacttc taaatcctca 4921 aatgtattat ctattgacgg ctctaatcta ttagttgtta gtgctcctaa agatatttta 4981 gataaccttc ctcaattcct ttcaactgtt gatttgccaa ctgaccagat attgattgag 5041 ggtttgatat ttgaggttca gcaaggtgat gctttagatt tttcatttgc tgctggctct 5101 cagcgtggca ctgttgcagg cggtgttaat actgaccgcc tcacctctgt tttatcttct 5161 gctggtggtt cgttcggtat ttttaatggc gatgttttag ggctatcagt tcgcgcatta 5221 aagactaata gccattcaaa aatattgtct gtgccacgta ttcttacgct ttcaggtcag 5281 aagggttcta tctctgttgg ccagaatgtc ccttttatta ctggtcgtgt gactggtgaa 5341 tctgccaatg taaataatcc atttcagacg attgagcgtc aaaatgtagg tatttccatg 5401 agcgtttttc ctgttgcaat ggctggcggt aatattgttc tggatattac cagcaaggcc 5461 gatagtttga gttcttctac tcaggcaagt gatgttatta ctaatcaaag aagtattgct 5521 acaacggtta atttgcgtga tggacagact cttttactcg gtggcctcac tgattataaa 5581 aacacttctc aggattctgg cgtaccgttc ctgtctaaaa tccctttaat cggcctcctg 5641 tttagctccc gctctgattc taacgaggaa agcacgttat acgtgctcgt caaagcaacc 5701 atagtacgcg ccctgtagcg gcgcattaag cgctagcagt gagcttcatg tggcaggaga 5761 aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg 5821 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg 5881 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg 5941 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa 6001 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct 6061 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc 6121 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg 6181 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt 6241 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga 6301 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt 6361 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac 6421 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag 6481 accaaaacga tctcaagaag atcatcttat taaggggtct gacgctcagt ggaacgaaaa 6541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 6601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 6661 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 6721 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 6781 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 6841 ccagccagcc gattcgagct cgcccgggga tcgaccagtt ggtgattttg aacttttgct 6901 ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa 6961 aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc tctgccagtg 7021 ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa 7081 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg 7141 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc 7201 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag 7261 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc 7321 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac 7381 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa 7441 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac 7501 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat 7561 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag 7621 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac 7681 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata 7741 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc 7801 atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat 7861 aacccccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt 7921 tttatcttgt gcaatgtaac atcagagatt ttgaaacaca acgtggcttt cccccccccc 7981 cccctgcagg tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 8041 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 8101 tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt cttattatca 8161 accggggtac at //