BBa_K1039021
type: ComponentDefinition
persistent identity: BBa K1039021
display id: BBa_K1039021
version: 1
was generated by: iGEM to SBOL conversion
description: Bxb1 integrase and Recombination Directionality Factor (RDF) Under ???Lock and Key??? Control with L
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:08:50Z
mutable provenance: BBa_I714036, BBa_R0011, BBa_J23119 and BBa_Q04121 were obtained from the iGEM 2013 distribution kit. The rest of the biobricks were synthesized by BioBasic Inc.
owned by: james
owned by: myers
mutable description: Bxb1 integrase (BBa_K1039003) and lacI repressor (BBa_Q04121) are under the constitutive strong promoter BBa_J23119. Key (BBa_I714036) and Bxb1 RDF (BBa_K1039006) are under the lac inducible promoter BBa_R0011. Bxb1 integrase (BBa_K1039003) and recombination directionality factor (RDF) (BBa_K1039006) with ribosome binding sites associated with the cis-repressive RNA ???lock??? (BBa_J01010), so translation is inhibited. The trans-activating RNA ???key??? (BBa_I714036) can displace the lock and allows translation. Together, Bxb1 integrase and RDF (BBa_K1039003 and BBa_K1039006) catalyze site-specific recombination between Bxb1 attR and attL sites (Bba_K1039033 and Bba_K1039034) They will also allow flipping of Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039004 from RL state to PB state. Lock and key regulate BXB1 integrase and BRDF expression. BXB1 integrase and BRDF???s expression is blocked by lock. Key expression is controlled by a lacI repressor, upon IPTG addition to the media, key will be expressed, which then unlocks BXB1 integrase and BRDF.
discontinued: false
dominant: false
group u list: _1346_
m user id: 0
owner id: 18339
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Bxb1 Integrase (BBa_K1039003) has the following three silent mutations, to remove forbidden restriction sites, base 273 G->A, base 1269 A->T and base 1440 G->A
star: false
creator: Jama Hagi-Yusuf
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J01010
component: BBa_Q04121
component: BBa_J23119
component: BBa_I714036
component: BBa_R0011
component: BBa_B0015
component: BBa_K1039003
component: BBa_K1039006
sequence annotation: BBa_J23119
sequence annotation: BBa_Q04121
sequence annotation: BBa_J01010
sequence annotation: BBa_K1039003
sequence annotation: BBa_B0015
sequence annotation: BBa_R0011
sequence annotation: BBa_I714036
sequence annotation: BBa_K1039006
sequence: https://synbiohub.org/public/igem/BBa_K1039021_sequence/1

BBa_J01010
type: Component
persistent identity: component2340565
display id: component2340565
version: 1
top level: BBa_K1039021
definition: Lock 1
access: public

BBa_Q04121
type: Component
persistent identity: component2340564
display id: component2340564
version: 1
top level: BBa_K1039021
definition: lacI
access: public

BBa_J23119
type: Component
persistent identity: component2340545
display id: component2340545
version: 1
top level: BBa_K1039021
definition: BBa_J23119
access: public

BBa_I714036
type: Component
persistent identity: component2340579
display id: component2340579
version: 1
top level: BBa_K1039021
definition: BBa_I714036
access: public

BBa_R0011
type: Component
persistent identity: component2340574
display id: component2340574
version: 1
top level: BBa_K1039021
definition: lacI+pL
access: public

BBa_B0015
type: Component
persistent identity: component2340573
display id: component2340573
version: 1
top level: BBa_K1039021
definition: BBa_B0015
access: public

BBa_K1039003
type: Component
persistent identity: component2340566
display id: component2340566
version: 1
top level: BBa_K1039021
definition: Bxb1-int
access: public

BBa_K1039006
type: Component
persistent identity: component2340594
display id: component2340594
version: 1
top level: BBa_K1039021
definition: BBa_K1039006
access: public

BBa_J01010
type: Component
persistent identity: component2340592
display id: component2340592
version: 1
top level: BBa_K1039021
definition: Lock 1
access: public

BBa_R0011
type: Component
persistent identity: component2340587
display id: component2340587
version: 1
top level: BBa_K1039021
definition: lacI+pL
access: public

BBa_B0015
type: Component
persistent identity: component2340586
display id: component2340586
version: 1
top level: BBa_K1039021
definition: BBa_B0015
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2340545
display id: annotation2340545
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340545/range2340545/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340545/range2340545/1
type: Range
persistent identity: range2340545
display id: range2340545
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2340564
display id: annotation2340564
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340564/range2340564/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340564/range2340564/1
type: Range
persistent identity: range2340564
display id: range2340564
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 44
end: 1414
orientation: inline

BBa_J01010
type: SequenceAnnotation
persistent identity: annotation2340565
display id: annotation2340565
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340565/range2340565/1
component: BBa_J01010

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340565/range2340565/1
type: Range
persistent identity: range2340565
display id: range2340565
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 1423
end: 1462
orientation: inline

BBa_K1039003
type: SequenceAnnotation
persistent identity: annotation2340566
display id: annotation2340566
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340566/range2340566/1
component: BBa_K1039003

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340566/range2340566/1
type: Range
persistent identity: range2340566
display id: range2340566
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 1469
end: 2971
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2340573
display id: annotation2340573
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340573/range2340573/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340573/range2340573/1
type: Range
persistent identity: range2340573
display id: range2340573
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 2980
end: 3108
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2340574
display id: annotation2340574
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340574/range2340574/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340574/range2340574/1
type: Range
persistent identity: range2340574
display id: range2340574
version: 1
top level: BBa_K1039021
direction: direction/forward
start: 3117
end: 3170
orientation: inline

BBa_I714036
type: SequenceAnnotation
persistent identity: annotation2340579
display id: annotation2340579
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340579/range2340579/1
component: BBa_I714036

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340579/range2340579/1
type: Range
persistent identity: range2340579
display id: range2340579
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 3180
end: 3273
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2340586
display id: annotation2340586
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340586/range2340586/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340586/range2340586/1
type: Range
persistent identity: range2340586
display id: range2340586
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 3282
end: 3410
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2340587
display id: annotation2340587
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340587/range2340587/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340587/range2340587/1
type: Range
persistent identity: range2340587
display id: range2340587
version: 1
top level: BBa_K1039021
direction: direction/forward
start: 3419
end: 3472
orientation: inline

BBa_J01010
type: SequenceAnnotation
persistent identity: annotation2340592
display id: annotation2340592
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340592/range2340592/1
component: BBa_J01010

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340592/range2340592/1
type: Range
persistent identity: range2340592
display id: range2340592
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 3482
end: 3521
orientation: inline

BBa_K1039006
type: SequenceAnnotation
persistent identity: annotation2340594
display id: annotation2340594
version: 1
top level: BBa_K1039021
location: https://synbiohub.org/public/igem/BBa_K1039021/annotation2340594/range2340594/1
component: BBa_K1039006

https://synbiohub.org/public/igem/BBa_K1039021/annotation2340594/range2340594/1
type: Range
persistent identity: range2340594
display id: range2340594
version: 1
top level: BBa_K1039021
direction: direction/reverse
start: 3528
end: 4295
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

Lock 1
type: ComponentDefinition
persistent identity: BBa J01010
display id: BBa_J01010
version: 1
was generated by: iGEM to SBOL conversion
description: Riboregulator Lock 1
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: Biobricked version of Isaacs' riboregulator cis repressed lock, crR12.
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01010_sequence/1

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_I714036
type: ComponentDefinition
persistent identity: BBa I714036
display id: BBa_I714036
version: 1
was generated by: iGEM to SBOL conversion
description: Riboregulator key 1
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:47Z
mutable provenance: J01008
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The same as J01008
discontinued: false
dominant: false
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The Isaacs key1 riboregulator taR12 is modeled on the hok/sok post-segregational killing method of plasmid R1. It makes 25 base pairs with its counterpart, the riboregulator J01010 (aka "lock 1" or "crR12"). Isaacs documentation (see reference #1): Upon induction of the araC promoter (using 0.25% arabinose solution), the taR12 key activated the corresponding crR12 lock to express the downstream indicator, GFP, to levels of fluorescence 19-fold greater than without taR12 expression. Maximal expression was reached at 70 minutes after addition of arabinose solution, and was maintained after that point.
star: false
creator: Chunbo Lou
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_I714036_sequence/1

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

lacI
type: ComponentDefinition
persistent identity: BBa Q04121
display id: BBa_Q04121
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0012.B0015.R0011)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LacI QPI with strong RBS, hybrid promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0015
component: BBa_C0012
component: BBa_R0011
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_Q04121_sequence/1

BBa_B0015
type: Component
persistent identity: component2221145
display id: component2221145
version: 1
top level: lacI
definition: BBa_B0015
access: public

BBa_C0012
type: Component
persistent identity: component2221136
display id: component2221136
version: 1
top level: lacI
definition: lacI
access: public

BBa_R0011
type: Component
persistent identity: component2221146
display id: component2221146
version: 1
top level: lacI
definition: lacI+pL
access: public

BBa_B0034
type: Component
persistent identity: component2221134
display id: component2221134
version: 1
top level: lacI
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221134
display id: annotation2221134
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221134/range2221134/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221134/range2221134/1
type: Range
persistent identity: range2221134
display id: range2221134
version: 1
top level: lacI
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2221136
display id: annotation2221136
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221136/range2221136/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221136/range2221136/1
type: Range
persistent identity: range2221136
display id: range2221136
version: 1
top level: lacI
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221145
display id: annotation2221145
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221145/range2221145/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221145/range2221145/1
type: Range
persistent identity: range2221145
display id: range2221145
version: 1
top level: lacI
direction: direction/reverse
start: 1180
end: 1308
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221146
display id: annotation2221146
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221146/range2221146/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221146/range2221146/1
type: Range
persistent identity: range2221146
display id: range2221146
version: 1
top level: lacI
direction: direction/forward
start: 1317
end: 1370
orientation: inline

Bxb1-int
type: ComponentDefinition
persistent identity: BBa K1039003
display id: BBa_K1039003
version: 1
was generated by: iGEM to SBOL conversion
description: Bxb1 integrase
created: 2013-07-07T11:00:00Z
modified: 2015-05-08T01:08:50Z
mutable provenance: Ghosh P, Kim AI, Hatfull GF. The orientation of mycobacteriophage Bxb1 inte- gration is solely dependent on the central dinucleotide of attP and attB. Molecular Cell 2003, 12:1101???1111.
owned by: james
owned by: myers
mutable description: In nature, serine integrases allow phages to integrate into the bacterial genome by site-specific recombination between attachment sites on the phage and bacterial genomes (attP and attB), both of which are between 30 and 60 bp depending which phage serine integrase is considered. Recombination between attP and attB leaves behind left and right attachment sites (attL and attR), which cannot be acted upon by Int alone. Thus, in the presence of Int alone, this recombination is irreversible (1).
discontinued: false
dominant: false
experience: experience/None
group u list: _1346_
m user id: 0
owner id: 14183
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: ...
star: false
creator: Aaron Bender
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1039003_sequence/1

BBa_K1039006
type: ComponentDefinition
persistent identity: BBa K1039006
display id: BBa_K1039006
version: 1
was generated by: iGEM to SBOL conversion
description: Bxb1 Recombination Directionality Factor
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:50Z
mutable provenance: The sequence was designed by Bonnet lab at the Department of Bioengineering, Stanford University, and was further modified by Waterloo team.
owned by: james
owned by: myers
mutable description: This is the recombination directionality factor (RDF) that acts with Bxb1 serine integrase. It reverses the recombination directionality of Bxb1 integrase (BBa_K1039003). In concert with Bxb1 RDF, Bxb1 integrase (BBa_K1039003) catalyzes site-specific recombination between Bxb1 attR and attL sites, to produce Bxb1 attP and attB sites. This recombination is uni-directional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of Bxb1 integrase and RDF. In concert with Bxb1 RDF (BBa_K1039006), Bxb1 integrase can be used to flip the Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039005 from RL state to PB state.
discontinued: false
dominant: false
group u list: _1346_
m user id: 0
owner id: 14649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: to be written later.
star: false
creator: Urooj Kishor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Bxb1 RDF
sequence: https://synbiohub.org/public/igem/BBa_K1039006_sequence/1

Bxb1 RDF
type: SequenceAnnotation
persistent identity: annotation2339258
display id: annotation2339258
version: 1
top level: BBa_K1039006
location: https://synbiohub.org/public/igem/BBa_K1039006/annotation2339258/range2339258/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1039006/annotation2339258/range2339258/1
type: Range
persistent identity: range2339258
display id: range2339258
version: 1
top level: BBa_K1039006
direction: direction/forward
start: 1
end: 768
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

https://synbiohub.org/public/igem/BBa_K1039021_sequence/1
type: Sequence
persistent identity: BBa K1039021 sequence
display id: BBa_K1039021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaactagaatcacctcttggatttgggtattaaagaggagatactagatgagagccctggtagtcatccgcctgtcccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggacgtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggcccggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggttgacccgatcgatccggcatctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacgacgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaaccgttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgtggacggggagtggcggctggtgccggacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01010_sequence/1
type: Sequence
persistent identity: BBa J01010 sequence
display id: BBa_J01010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttggatttgggtattaaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I714036_sequence/1
type: Sequence
persistent identity: BBa I714036 sequence
display id: BBa_I714036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaatccaggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04121_sequence/1
type: Sequence
persistent identity: BBa Q04121 sequence
display id: BBa_Q04121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1039003_sequence/1
type: Sequence
persistent identity: BBa K1039003 sequence
display id: BBa_K1039003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagagccctggtagtcatccgcctgtcccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggacgtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggcccggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggttgacccgatcgatccggcatctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacgacgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaaccgttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgtggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtatcaccgcgtcgtcgacaaccacgagccgctgcacctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaaggactacttcgcgcagctgcaaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctccgaggcgatgctcgggtacgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgagccgatcctgacccgtgagcagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtctaccccgtcgctgctgctgcgggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaagcacccgcgctaccgctgccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggacgcgttctgcgaggagcaggtgctggatctgctcggggacgcggagcgtctggagaaagtctgggtagccggctcggactccgcggtcgaactcgcggaggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggccggctctccgcagcgagaagcactggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggtcttgaggctcgcccgtctggctgggagtggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaagaacacctggcttcggtcgatgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcggggatctgcaagagtacgagcagcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1039006_sequence/1
type: Sequence
persistent identity: BBa K1039006 sequence
display id: BBa_K1039006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactcagcgtatcgtctttctacccgatactcagttgcctttcgaggcgcgcaaagagatgcaagcggtcatccgcttcatcggggatgtccagccgtacggcgtggtacatatcggtgacgtcctagacctgccgcagccctcgcgctggaaccggggaaccaagggcgagttcgagggctcggtgtaccgcgacgcggactacgccaagaagaacctgatggagccgctgcgcaaggtctacgacggctggatcgggatgcacgagggcaaccacgatctgcgagcccgcgagtacctggccaagaacgcaccggccctggagggtacgcacgctttcgacatcgacgtgctgctcgacttcgacgggttcggtgtggagctgctgcctgacttctacgacatcgctccgggctggatctccactcacgggcacatgggcaagatgacgctatcccagatcgccggatcgacagcgctcaacggtgccaagaagttcggcaagtccgtggtctgcggccacacgcaccggcaggctgtcgtctcgcactcgttcgggtacggcggctcggtgcgcaagaccgtcaccggcatggaagtcgggcacctgatggacatgaagaaggccaactatctaaagggcggagccgggaactggcagatgggcttcgggatgctcacggtcgacggcaagcatgtcaaggctgagatcgtcccgatcctgggaggcaagttcaccgttgacggccaggtctgggaagtctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z