LOCUS BBa_K1039022 4705 bp DNA linear 14-May-2024 DEFINITION PhiC31 integrase and PhiC31 RDF under lock and key control with lacI Expression Cassette ACCESSION BBa_K1039022 VERSION BBa_K1039022.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1151..1189 /label=LVA CDS 62..1189 /label=lacI-LVA misc_feature 62..1189 /label=BBa_C0012 misc_feature 1190..1214 /label=Help:Barcodes CDS 62..1189 /label=BBa_C0012 stem_loop 1234..1277 /label=stem_loop misc_feature 1223..1302 /label=BBa_B0010 terminator 1223..1302 /label=BBa_B0010 stem_loop 1318..1337 /label=T7 TE misc_feature 1344..1344 /label=stop polyA_site 1338..1351 /label=polya misc_feature 1311..1351 /label=BBa_B0012 terminator 1311..1351 /label=BBa_B0012 terminator 1223..1351 /label=BBa_B0015 misc_feature 1362..1378 /label=lac O1 promoter 1379..1384 /label=-35 misc_feature 1385..1401 /label=lac O1 promoter 1402..1407 /label=-10 misc_feature 1360..1413 /label=BBa_R0011 promoter 1360..1413 /label=BBa_R0011 misc_feature 44..1414 /label=BBa_Q04121 misc_feature 1423..1462 /label=BBa_J01010 misc_feature 1469..3413 /label=PhiC31 integrase CDS 1469..3413 /label=BBa_K1039012 stem_loop 3433..3476 /label=stem_loop misc_feature 3422..3501 /label=BBa_B0010 terminator 3422..3501 /label=BBa_B0010 stem_loop 3517..3536 /label=T7 TE misc_feature 3543..3543 /label=stop polyA_site 3537..3550 /label=polya misc_feature 3510..3550 /label=BBa_B0012 terminator 3510..3550 /label=BBa_B0012 terminator 3422..3550 /label=BBa_B0015 misc_feature 3561..3577 /label=lac O1 promoter 3578..3583 /label=-35 misc_feature 3584..3600 /label=lac O1 promoter 3601..3606 /label=-10 misc_feature 3559..3612 /label=BBa_R0011 promoter 3559..3612 /label=BBa_R0011 misc_feature 3622..3715 /label=BBa_I714036 stem_loop 3735..3778 /label=stem_loop misc_feature 3724..3803 /label=BBa_B0010 terminator 3724..3803 /label=BBa_B0010 stem_loop 3819..3838 /label=T7 TE misc_feature 3845..3845 /label=stop polyA_site 3839..3852 /label=polya misc_feature 3812..3852 /label=BBa_B0012 terminator 3812..3852 /label=BBa_B0012 terminator 3724..3852 /label=BBa_B0015 misc_feature 3863..3879 /label=lac O1 promoter 3880..3885 /label=-35 misc_feature 3886..3902 /label=lac O1 promoter 3903..3908 /label=-10 misc_feature 3861..3914 /label=BBa_R0011 promoter 3861..3914 /label=BBa_R0011 misc_feature 3924..3963 /label=BBa_J01010 CDS 3970..4705 /label=BBa_K1039013 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt 1381 gacattgtga gcggataaca agatactgag cacatactag agaactagaa tcacctcttg 1441 gatttgggta ttaaagagga gatactagat ggacacgtac gcgggtgctt acgaccgtca 1501 gtcgcgcgag cgcgagaact cgagcgcagc aagcccagcg acacagcgta gcgccaacga 1561 agacaaggcg gccgaccttc agcgcgaagt cgagcgcgac gggggccggt tcaggttcgt 1621 cgggcatttc agcgaagcgc cgggcacgtc ggcgttcggg acggcggagc gcccggagtt 1681 cgaacgcatc ctgaacgaat gccgcgccgg gcggctcaac atgatcattg tctatgacgt 1741 gtcgcgcttc tcgcgcctga aggtcatgga cgcgattccg attgtctcgg aattgctcgc 1801 cctgggcgtg acgattgttt ccactcagga aggcgtcttc cggcagggaa acgtcatgga 1861 cctgattcac ctgattatgc ggctcgacgc gtcgcacaaa gaatcttcgc tgaagtcggc 1921 gaagattctc gacacgaaga accttcagcg cgaattgggc gggtacgtcg gcgggaaggc 1981 gccttacggc ttcgagcttg tttcggagac gaaggagatc acgcgcaacg gccgaatggt 2041 caatgtcgtc atcaacaagc ttgcgcactc gaccactccc cttaccggac ccttcgagtt 2101 cgagcccgac gtaatccggt ggtggtggcg tgagatcaag acgcacaaac accttccctt 2161 caagccgggc agtcaagccg ccattcaccc gggcagcatc acggggcttt gtaagcgcat 2221 ggacgctgac gccgtgccga cccggggcga gacgattggg aagaagaccg cttcaagcgc 2281 ctgggacccg gcaaccgtta tgcgaatcct tcgggacccg cgtattgcgg gcttcgccgc 2341 tgaggtgatc tacaagaaga agccggacgg cacgccgacc acgaagattg agggttaccg 2401 cattcagcgc gacccgatca cgctccggcc ggtcgagctt gattgcggac cgatcatcga 2461 gcccgctgag tggtatgagc ttcaggcgtg gttggacggc agggggcgcg gcaaggggct 2521 ttcccggggg caagccattc tgtccgccat ggacaagctg tactgcgagt gtggcgccgt 2581 catgacttcg aagcgcgggg aagaatcgat caaggactct taccgctgcc gtcgccggaa 2641 ggtggtcgac ccgtccgcac ctgggcagca cgaaggcacg tgcaacgtca gcatggcggc 2701 actcgacaag ttcgttgcgg aacgcatctt caacaagatc aggcacgccg aaggcgacga 2761 agagacgttg gcgcttctgt gggaagccgc ccgacgcttc ggcaagctca ctgaggcgcc 2821 tgagaagagc ggcgaacggg cgaaccttgt tgcggagcgc gccgacgccc tgaacgccct 2881 tgaagagctg tacgaagacc gcgcggcagg cgcgtacgac ggacccgttg gcaggaagca 2941 cttccggaag caacaggcag cgctgacgct ccggcagcaa ggggcggaag agcggcttgc 3001 cgaacttgaa gccgccgaag ccccgaagct tccccttgac caatggttcc ccgaagacgc 3061 cgacgctgac ccgaccggcc ctaagtcgtg gtgggggcgc gcgtcagtag acgacaagcg 3121 cgtgttcgtc gggctcttcg tagacaagat cgttgtcacg aagtcgacta cgggcagggg 3181 gcagggaacg cccatcgaga agcgcgcttc gatcacgtgg gcgaagccgc cgaccgacga 3241 cgacgaagac gacgcccagg acggcacgga agacgtagcg gcgtagcgag acacccggga 3301 agcctgttag gcgctgagac gggcgcacag cgggcttcct ggggcagcgg gaagggtcgg 3361 ccggtccccc ggtcggccca tttctcttgt ctcggtttag ttagttagcc taatactaga 3421 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 3481 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 3541 tgcgtttata tactagagaa ttgtgagcgg ataacaattg acattgtgag cggataacaa 3601 gatactgagc acatactaga gacccaaatc caggaggtga atctagtagg tggttaatga 3661 aaattaactt acttactaga aatatctcta aaaagccaga ttattaatcc ggctttacta 3721 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 3781 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 3841 tctgcgttta tatactagag aattgtgagc ggataacaat tgacattgtg agcggataac 3901 aagatactga gcacatacta gagaactaga atcacctctt ggatttgggt attaaagagg 3961 agatactaga tggcgaagcg ttcgatctgg gccggagacg aagacaacaa gccgaagaag 4021 cgtgagacgt acgccgacga caccgttggg cgtttccact ccggctacag cgagacgaat 4081 gagcggggca aggtcgttcc cgtcgcgctc gacaagtggc gtatctcgac cggcgaacag 4141 tccgttgcgg acgctgtggc tcagctcttc ggcggcaccc cggttgagaa cgaagagagc 4201 acgtctgaga acttcatcga cgtgttcacc gaccggccga aggtccccgt gatcattgag 4261 gctgacggta tccactggga tatgaagctt tggctgaacg gcaagctgaa gcaccactgc 4321 gacggctttg acttcgtgtc gcacgccgac gaagagatga tcggtcagcc gtgcgggtgc 4381 ccgaagctct tcgatgagcg gaaggcagcg gcgaaggagt acgacgcgcc gaacccggca 4441 atcaccgtga ccttcacgct cgccgacgac ccggagcttg gccgcttcaa gttccagacc 4501 ggttcttgga cgctcttcaa ggttctgcac gaagccgaag acgacgttga gcgcgtcggc 4561 aagggtggcg ctgtgctcgc caatctcgaa cttgagcttg tggagtacac gccgaagcgt 4621 ggcccgatgc ggaacaagct tgtgagctac tacaagccga ccatcacggt tctgaagtcg 4681 tacaacgacg cgattgccga ctagg //