LOCUS BBa_K1039023 3410 bp DNA linear 13-May-2024 DEFINITION BXB1 integrase under lock and key control with lacI expression cassette ACCESSION BBa_K1039023 VERSION BBa_K1039023.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1151..1189 /label=LVA CDS 62..1189 /label=lacI-LVA misc_feature 62..1189 /label=BBa_C0012 misc_feature 1190..1214 /label=Help:Barcodes CDS 62..1189 /label=BBa_C0012 stem_loop 1234..1277 /label=stem_loop misc_feature 1223..1302 /label=BBa_B0010 terminator 1223..1302 /label=BBa_B0010 stem_loop 1318..1337 /label=T7 TE misc_feature 1344..1344 /label=stop polyA_site 1338..1351 /label=polya misc_feature 1311..1351 /label=BBa_B0012 terminator 1311..1351 /label=BBa_B0012 terminator 1223..1351 /label=BBa_B0015 misc_feature 1362..1378 /label=lac O1 promoter 1379..1384 /label=-35 misc_feature 1385..1401 /label=lac O1 promoter 1402..1407 /label=-10 misc_feature 1360..1413 /label=BBa_R0011 promoter 1360..1413 /label=BBa_R0011 misc_feature 44..1414 /label=BBa_Q04121 misc_feature 1423..1462 /label=BBa_J01010 CDS 1469..2971 /label=BBa_K1039003 stem_loop 2991..3034 /label=stem_loop misc_feature 2980..3059 /label=BBa_B0010 terminator 2980..3059 /label=BBa_B0010 stem_loop 3075..3094 /label=T7 TE misc_feature 3101..3101 /label=stop polyA_site 3095..3108 /label=polya misc_feature 3068..3108 /label=BBa_B0012 terminator 3068..3108 /label=BBa_B0012 terminator 2980..3108 /label=BBa_B0015 misc_feature 3119..3135 /label=lac O1 promoter 3136..3141 /label=-35 misc_feature 3142..3158 /label=lac O1 promoter 3159..3164 /label=-10 misc_feature 3117..3170 /label=BBa_R0011 promoter 3117..3170 /label=BBa_R0011 misc_feature 3180..3273 /label=BBa_I714036 stem_loop 3293..3336 /label=stem_loop misc_feature 3282..3361 /label=BBa_B0010 terminator 3282..3361 /label=BBa_B0010 stem_loop 3377..3396 /label=T7 TE misc_feature 3403..3403 /label=stop polyA_site 3397..3410 /label=polya misc_feature 3370..3410 /label=BBa_B0012 terminator 3370..3410 /label=BBa_B0012 terminator 3282..3410 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt 1381 gacattgtga gcggataaca agatactgag cacatactag agaactagaa tcacctcttg 1441 gatttgggta ttaaagagga gatactagat gagagccctg gtagtcatcc gcctgtcccg 1501 cgtcaccgat gctacgactt caccggagcg tcagctggag tcttgccagc agctctgcgc 1561 ccagcgcggc tgggacgtcg tcggggtagc ggaggatctg gacgtctccg gggcggtcga 1621 tccgttcgac cggaagcgca gaccgaacct ggcccggtgg ctagcgttcg aggagcaacc 1681 gttcgacgtg atcgtggcgt accgggtaga ccggttgacc cgatcgatcc ggcatctgca 1741 acagctggtc cactgggccg aggaccacaa gaagctggtc gtctccgcga ccgaagcgca 1801 cttcgatacg acgacgccgt ttgcggcggt cgtcatcgcg cttatgggaa cggtggcgca 1861 gatggaatta gaagcgatca aagagcggaa ccgttcggct gcgcatttca atatccgcgc 1921 cgggaaatac cgaggatccc tgccgccgtg gggatacctg cctacgcgcg tggacgggga 1981 gtggcggctg gtgccggacc ctgtgcagcg agagcgcatc ctcgaggtgt atcaccgcgt 2041 cgtcgacaac cacgagccgc tgcacctggt ggcccacgac ctgaaccggc gtggtgtcct 2101 gtcgccgaag gactacttcg cgcagctgca aggccgcgag ccgcagggcc gggagtggtc 2161 ggctaccgcg ctgaagcgat cgatgatctc cgaggcgatg ctcgggtacg cgactctgaa 2221 cggtaagacc gtccgagacg acgacggagc cccgctggtg cgggctgagc cgatcctgac 2281 ccgtgagcag ctggaggcgc tgcgcgccga gctcgtgaag acctcccggg cgaagcccgc 2341 ggtgtctacc ccgtcgctgc tgctgcgggt gttgttctgc gcggtgtgcg gggagcccgc 2401 gtacaagttc gccgggggag gacgtaagca cccgcgctac cgctgccgct cgatggggtt 2461 cccgaagcac tgcgggaacg gcacggtggc gatggccgag tgggacgcgt tctgcgagga 2521 gcaggtgctg gatctgctcg gggacgcgga gcgtctggag aaagtctggg tagccggctc 2581 ggactccgcg gtcgaactcg cggaggtgaa cgcggagctg gtggacctga cgtcgctgat 2641 cggctccccg gcctaccggg ccggctctcc gcagcgagaa gcactggatg cccgtattgc 2701 ggcgctggcc gcgcggcaag aggagctgga gggtcttgag gctcgcccgt ctggctggga 2761 gtggcgcgag accgggcagc ggttcgggga ctggtggcgg gagcaggaca ccgcggcaaa 2821 gaacacctgg cttcggtcga tgaacgttcg gctgacgttc gacgtccgcg gcgggctgac 2881 tcgcacgatc gacttcgggg atctgcaaga gtacgagcag catctcaggc tcggcagcgt 2941 ggtcgaacgg ctacacaccg ggatgtcgta gtactagagc caggcatcaa ataaaacgaa 3001 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3061 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 3121 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga 3181 cccaaatcca ggaggtgaat ctagtaggtg gttaatgaaa attaacttac ttactagaaa 3241 tatctctaaa aagccagatt attaatccgg ctttactaga gccaggcatc aaataaaacg 3301 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3361 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //