LOCUS       BBa_K1040008             875 bp    DNA     linear       22-Dec-2024
DEFINITION  toggle switch construction kit ( Tet promoter_rbs_C1 represser_rbs 
            )
ACCESSION   BBa_K1040008
VERSION     BBa_K1040008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..791
                     /label=cI lambda
     misc_feature    792..824
                     /label=LVA
     misc_feature    831..855
                     /label=Help:Barcodes
     CDS             81..855
                     /label=BBa_C0051
     misc_feature    868..871
                     /label=conserved
     RBS             864..875
                     /label=BBa_B0034
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg
      121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc
      181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg
      241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg
      301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta
      361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga
      421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa
      481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac
      541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg
      601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga
      661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga
      721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag
      781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt
      841 gctagtgtag atcgctacta gagaaagagg agaaa
//