LOCUS       BBa_K104001             2122 bp    DNA     linear       04-May-2024
DEFINITION  Sensor for small peptide Subtilin
ACCESSION   BBa_K104001
VERSION     BBa_K104001.1  
FEATURES             Location/Qualifiers
     promoter        7..123
                     /label=Prom_spaRK
     RBS             124..132
                     /label=RBS_spaR
     CDS             137..799
                     /label=spaR
     RBS             772..780
                     /label=RBS_spaK
     CDS             772..1953
                     /label=spaK
     stem_loop       1939..1957
                     /label=ter_rrn0
     promoter        2043..2120
                     /label=Prom_spaS
ORIGIN
        1 ctagagatcc gcatgaaata aattcagggg tattgatgat ggtttttggt ataatatgta
       61 caatcattgc cagtcttata tggtttagta aatgggaagg cagaaaaacg tttgaatagt
      121 atgagggggc cgggaaatgg caaaaatcct tgctgtcgat gatgaaaaag atattcttgt
      181 actgatacaa aatattttgc gccgtgacca gcatcaagtt gacattttag atcatgttca
      241 tggacagccg cctgatgtat ttcaaggata tgatttaatt ttgttagatg tgatgatgcc
      301 ggatattgac ggttttgagt tatgtaaaca gatacggcct ttggttgatt gtcccatttt
      361 attcctcaca gctaaaacgg aagaggaagc gatagtaaaa ggcctcatta ccggcggaga
      421 tgattatatt acaaagccgt tcggcgttag agagcttagt gccagagtga atgctcactt
      481 aagaagagaa cggagagata aacatcaatc gaagagagtg atctccggtt ttttatttca
      541 ttttgatagt aaagaagtat ttatcaataa caacaaactc aacctaacaa aaaatgaata
      601 taaaatctgt gaatttttag ctcaacacaa agggagaact ttttcgagag aacaaattta
      661 cgaggagata tatggattag aggggaatgc tttgtattca acaattactg aatttattcg
      721 aacgattcga aaaaaatgta aagagcataa tgcagatccc attaaaacgg tatggggcgt
      781 tgggtacaaa tgggaatagg ttttaagggc cggaaaacgc ttcttagaga actggtaaag
      841 tatatggtga ccctatgtat tagcttggtc gtacttgctc tgctatatat atttataaat
      901 acgattgcaa tgaatacagg tttctcacac ccagcgaatt ataatgaacg agaagcggaa
      961 aaacttgcgc cgaagctgga aaccatcgac aaagtaactg ccgatatgat tcctgataca
     1021 atgtcatatg ccattcttaa caaagaaaca aaacaaaaaa cagctggaac tattaaagaa
     1081 aaagacctac agctggtaaa aaagaagatt gaaaaaaaac catatgtcaa ttataagcaa
     1141 aaaggctatt tggttataga aagaaacaat gaatattgtg ttttgcaata ttcattgcgt
     1201 gccgatttca gttcgccttt actaagaaaa taccttccta attatgaatt aacgagtatt
     1261 tgtattttga ttatcttgct cattatagtt atctctatta taacaactta ctttgcgaat
     1321 agattaagaa aacattttga gacattgaac gtaatcactc gatatataaa agagcaaaat
     1381 ttgcaattca ctcccgaatt tacacatatt aaggaatttg atgatgtgat tgactcgttg
     1441 attgaaatga gagatgctct ccagtcctct cttgaggctc tgtggcgttt agaaaaaaac
     1501 aagaaagaac aaataggtgc tttggcacat gagatcaaga ttccgatcac cattattaag
     1561 gggaatgctg aattactcag cttgtcaatg caaaacgaag aacaagctga gtatacaaag
     1621 tatattcttg gagcaggaaa tcaaattgaa cagtatattt atcagctgat tcatctttcg
     1681 aaaacggagg atgccttaac gattcatttg gagaaagcat ctgtagatga gttgacagaa
     1741 actctagtga aagatatatc cgcctataaa ggaaataaaa atataaacat ttcgttcaaa
     1801 aaagaaaatc taatgaaaga agcgaaaata gactggcaat tgttacatag ggcattattg
     1861 aatattttaa caaatgcagt tgattataca ccggaaggcg gcaccgtctc ggtacatgca
     1921 gaatcgattc tgagatattt tatttttttg taataaagga cgccgccaag ccagcttaaa
     1981 cccagctcaa tgagctgggt tttttgtttg ttaaaaatga agaagaaact gtgaagcgta
     2041 ttgatcttaa aaaaaggaaa aaattgataa aatcttgata tttgtctgtt actatttagg
     2101 tattgaaagg aggtgaccac ct
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