BBa_K104001 1 Subtilin P Sensor for small peptide Subtilin 2008-10-23T11:00:00Z 2015-06-09T09:00:20Z The two component system, its promoter and RBS's, and the pSpaS promoter sequence are all derived from the genome of Bacillus subtilis ATCC 6633. This part encodes a two-component system that can be used to sense the level of a small lantibiotic peptide called subtilin. It belongs to a series of parts that can be used to sense quorum sensing peptides from Gram positive organisms. In the Newcastle 2008 iGEM entry it was used a 'proof of concept' brick to test whether a peptide produced by one organism could be sensed by a different organism. The part contains two orfs arranged in an operon, SpaK and SpaR, that are driven by their native RBSs and the promoter PspaRK. Downstream of the SpaRK operon is terminator followed by a RBS and a promoter PspaS. In response to subtilin, the sensor kinase SpaK will phosphorylate, and hence activate, the response regulator SpaR. The phosphorylated SpaR protein activates the PspaS promoter driving the expression of any downstream genes. In this brick there are no ORF's downstream of PspaS but the brick has been tested with gfp and mcherry by the Newcastle 2008 team. false false _237_ 4206 2652 9 Not in stock false A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified. false The Newcastle 2008 iGEM team annotation1999038 1 spaK range1999038 1 772 1953 annotation1999037 1 RBS_spaK range1999037 1 772 780 annotation1999039 1 ter_rrn0 range1999039 1 1939 1957 annotation1986985 1 Prom_spaRK range1986985 1 7 123 annotation1999035 1 RBS_spaR range1999035 1 124 132 annotation1999036 1 spaR range1999036 1 137 799 annotation1999040 1 Prom_spaS range1999040 1 2043 2120 BBa_K104001_sequence 1 ctagagatccgcatgaaataaattcaggggtattgatgatggtttttggtataatatgtacaatcattgccagtcttatatggtttagtaaatgggaaggcagaaaaacgtttgaatagtatgagggggccgggaaatggcaaaaatccttgctgtcgatgatgaaaaagatattcttgtactgatacaaaatattttgcgccgtgaccagcatcaagttgacattttagatcatgttcatggacagccgcctgatgtatttcaaggatatgatttaattttgttagatgtgatgatgccggatattgacggttttgagttatgtaaacagatacggcctttggttgattgtcccattttattcctcacagctaaaacggaagaggaagcgatagtaaaaggcctcattaccggcggagatgattatattacaaagccgttcggcgttagagagcttagtgccagagtgaatgctcacttaagaagagaacggagagataaacatcaatcgaagagagtgatctccggttttttatttcattttgatagtaaagaagtatttatcaataacaacaaactcaacctaacaaaaaatgaatataaaatctgtgaatttttagctcaacacaaagggagaactttttcgagagaacaaatttacgaggagatatatggattagaggggaatgctttgtattcaacaattactgaatttattcgaacgattcgaaaaaaatgtaaagagcataatgcagatcccattaaaacggtatggggcgttgggtacaaatgggaataggttttaagggccggaaaacgcttcttagagaactggtaaagtatatggtgaccctatgtattagcttggtcgtacttgctctgctatatatatttataaatacgattgcaatgaatacaggtttctcacacccagcgaattataatgaacgagaagcggaaaaacttgcgccgaagctggaaaccatcgacaaagtaactgccgatatgattcctgatacaatgtcatatgccattcttaacaaagaaacaaaacaaaaaacagctggaactattaaagaaaaagacctacagctggtaaaaaagaagattgaaaaaaaaccatatgtcaattataagcaaaaaggctatttggttatagaaagaaacaatgaatattgtgttttgcaatattcattgcgtgccgatttcagttcgcctttactaagaaaataccttcctaattatgaattaacgagtatttgtattttgattatcttgctcattatagttatctctattataacaacttactttgcgaatagattaagaaaacattttgagacattgaacgtaatcactcgatatataaaagagcaaaatttgcaattcactcccgaatttacacatattaaggaatttgatgatgtgattgactcgttgattgaaatgagagatgctctccagtcctctcttgaggctctgtggcgtttagaaaaaaacaagaaagaacaaataggtgctttggcacatgagatcaagattccgatcaccattattaaggggaatgctgaattactcagcttgtcaatgcaaaacgaagaacaagctgagtatacaaagtatattcttggagcaggaaatcaaattgaacagtatatttatcagctgattcatctttcgaaaacggaggatgccttaacgattcatttggagaaagcatctgtagatgagttgacagaaactctagtgaaagatatatccgcctataaaggaaataaaaatataaacatttcgttcaaaaaagaaaatctaatgaaagaagcgaaaatagactggcaattgttacatagggcattattgaatattttaacaaatgcagttgattatacaccggaaggcggcaccgtctcggtacatgcagaatcgattctgagatattttatttttttgtaataaaggacgccgccaagccagcttaaacccagctcaatgagctgggttttttgtttgttaaaaatgaagaagaaactgtgaagcgtattgatcttaaaaaaaggaaaaaattgataaaatcttgatatttgtctgttactatttaggtattgaaaggaggtgaccacct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z