LOCUS       BBa_K1040013            1989 bp    DNA     linear       23-May-2024
DEFINITION  ilvE mesurement system
ACCESSION   BBa_K1040013
VERSION     BBa_K1040013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..892
                     /label=amilCP
     CDS             227..895
                     /label=BBa_K592009
     misc_feature    908..911
                     /label=conserved
     RBS             904..915
                     /label=BBa_B0034
     CDS             922..1852
                     /label=BBa_K1040000
     misc_feature    904..1852
                     /label=BBa_K1040005
     stem_loop       1872..1915
                     /label=stem_loop
     misc_feature    1861..1940
                     /label=BBa_B0010
     terminator      1861..1940
                     /label=BBa_B0010
     stem_loop       1956..1975
                     /label=T7 TE
     misc_feature    1982..1982
                     /label=stop
     polyA_site      1976..1989
                     /label=polya
     misc_feature    1949..1989
                     /label=BBa_B0012
     terminator      1949..1989
                     /label=BBa_B0012
     terminator      1861..1989
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gtgtgatcgc
      241 taaacaaatg acctacaagg tttatatgtc aggcacggtc aatggacact actttgaggt
      301 cgaaggcgat ggaaaaggta agccctacga gggggagcag acggtaaagc tcactgtcac
      361 caagggcgga cctctgccat ttgcttggga tattttatca ccacagtgtc agtacggaag
      421 cataccattc accaagtacc ctgaagacat ccctgactat gtaaagcagt cattcccgga
      481 gggctataca tgggagagga tcatgaactt tgaagatggt gcagtgtgta ctgtcagcaa
      541 tgattccagc atccaaggca actgtttcat ctaccatgtc aagttctctg gtttgaactt
      601 tcctcccaat ggacctgtca tgcagaagaa gacacagggc tgggaaccca acactgagcg
      661 tctctttgca cgagatggaa tgctgctagg aaacaacttt atggctctga agttagaagg
      721 aggcggtcac tatttgtgtg aatttaaaac tacttacaag gcaaagaagc ctgtgaagat
      781 gccagggtat cactatgttg accgcaaact ggatgtaacc aatcacaaca aggattacac
      841 ttcggttgag cagtgtgaaa tttccattgc acgcaaacct gtggtcgcct aataatacta
      901 gagaaagagg agaaatacta gatgaccacg aagaaagctg attacatttg gttcaatggg
      961 gagatggttc gctgggaaga cgcgaaggtg catgtgatgt cgcacgcgct gcactatggc
     1021 acttcggttt ttgaaggcat ccgttgctac gactcgcaca aaggaccggt tgtattccgc
     1081 catcgtgagc atatgcagcg tctgcatgac tccgccaaaa tctatcgctt cccggtttcg
     1141 cagagcattg atgagctgat ggaagcttgt cgtgacgtga tccgcaaaaa caatctcacc
     1201 agcgcctata tccgtccgct gatcttcgtc ggtgatgttg gcatgggagt aaacccgcca
     1261 gcgggatact caaccgacgt gattatcgct gctttcccgt ggggagcgta tctgggcgca
     1321 gaagcgctgg agcaggggat cgatgcgatg gtttcctcct ggaaccgcgc agcaccaaac
     1381 accatcccga cggcggcaaa agccggtggt aactacctct cttccctgct ggtgggtagc
     1441 gaagcgcgcc gccacggtta tcaggaaggt atcgcgctgg atgtgaacgg ttatatctct
     1501 gaaggcgcag gcgaaaacct gtttgaagtg aaagatggtg tgctgttcac cccaccgttc
     1561 acctcctccg cgctgccggg tattacccgt gatgccatca tcaaactggc gaaagagctg
     1621 ggaattgaag tacgtgagca ggtgctgtcg cgcgaatccc tgtacctggc ggatgaagtg
     1681 tttatgtccg gtacggcggc agaaatcacg ccagtgcgca gcgtagacgg tattcaggtt
     1741 ggcgaaggcc gttgtggccc ggttaccaaa cgcattcagc aagccttctt cggcctcttc
     1801 actggcgaaa ccgaagataa atggggctgg ttagatcaag ttaatcaata aatactagag
     1861 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1921 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1981 gcgtttata
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