BBa_K1041000 1 BBa_K1041000 RFP Coding Device 2013-08-07T11:00:00Z 2015-05-08T01:08:51Z BBa_J04450 Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid. false false _1348_ 0 17128 9 In stock true Nde1 site was added at the start of the RFP coding region. false NRP UEA annotation2331501 1 end of LacI coding region (inactive) range2331501 1 1 88 annotation2331511 1 BBa_E1010 range2331511 1 227 932 annotation2331515 1 T7 TE range2331515 1 1036 1055 annotation2331510 1 Help:Barcodes range2331510 1 908 932 annotation2331500 1 BBa_R0010 range2331500 1 1 200 annotation2331523 1 Nde1 Restriction Site range2331523 1 224 229 annotation2331508 1 BBa_B0034 range2331508 1 209 220 annotation2331506 1 start range2331506 1 173 173 annotation2331516 1 polya range2331516 1 1056 1069 annotation2331512 1 BBa_B0010 range2331512 1 941 1020 annotation2331502 1 CAP binding site range2331502 1 89 126 annotation2331509 1 mrfp1 range2331509 1 227 901 annotation2331505 1 LacI binding site range2331505 1 166 200 annotation2331504 1 -10 range2331504 1 161 166 annotation2331503 1 -35 range2331503 1 137 142 annotation2331514 1 BBa_B0012 range2331514 1 1029 1069 annotation2331513 1 stem_loop range2331513 1 952 995 annotation2331507 1 conserved range2331507 1 213 216 annotation2331517 1 stop range2331517 1 1062 1062 annotation2331518 1 BBa_B0015 range2331518 1 941 1069 BBa_K1041000_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaataccatatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z