##gff-version 3
##sequence-region BBa_K1041000 1 1069
BBa_K1041000	.	engineered_region	227	932	.	+	0	ID=annotation2331511;Name=BBa_E1010
BBa_K1041000	.	engineered_tag	908	932	.	+	0	ID=annotation2331510;Name=Help:Barcodes
BBa_K1041000	.	engineered_region	209	220	.	+	0	ID=annotation2331508;Name=BBa_B0034
BBa_K1041000	.	engineered_region	1	200	.	+	0	ID=annotation2331500;Name=BBa_R0010
BBa_K1041000	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation2331502;Name=CAP binding site
BBa_K1041000	.	promoter	137	142	.	+	0	ID=annotation2331503;Name=-35
BBa_K1041000	.	CDS	1	88	.	+	0	ID=annotation2331501;Name=end of LacI coding region (inactive)
BBa_K1041000	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation2331505;Name=LacI binding site
BBa_K1041000	.	CDS	227	901	.	+	0	ID=annotation2331509;Name=mrfp1
BBa_K1041000	.	sequence_feature	224	229	.	+	0	ID=annotation2331523;Name=Nde1 Restriction Site
BBa_K1041000	.	engineered_region	941	1020	.	+	0	ID=annotation2331512;Name=BBa_B0010
BBa_K1041000	.	promoter	161	166	.	+	0	ID=annotation2331504;Name=-10
BBa_K1041000	.	stem_loop	1036	1055	.	+	0	ID=annotation2331515;Name=T7 TE
BBa_K1041000	.	engineered_region	941	1069	.	+	0	ID=annotation2331518;Name=BBa_B0015
BBa_K1041000	.	polyA_site	1056	1069	.	+	0	ID=annotation2331516;Name=polya
BBa_K1041000	.	start_codon	173	173	.	+	0	ID=annotation2331506;Name=start
BBa_K1041000	.	stop_codon	1062	1062	.	+	0	ID=annotation2331517;Name=stop
BBa_K1041000	.	engineered_region	1029	1069	.	+	0	ID=annotation2331514;Name=BBa_B0012
BBa_K1041000	.	conserved_region	213	216	.	+	0	ID=annotation2331507;Name=conserved
BBa_K1041000	.	stem_loop	952	995	.	+	0	ID=annotation2331513;Name=stem_loop
>BBa_K1041000
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaataccatatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata