BBa_K1041001 1 BBa_K1041001 Neomycin Resistance Coding Device + AntG promoter 2013-08-07T11:00:00Z 2015-05-08T01:08:51Z BBa_J04450 Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid. false false _1348_ 0 11711 9 In stock true Nde1 site was added at the start of the RFP coding region. false NRP UEA annotation2331525 1 rbs range2331525 1 113 121 annotation2331522 1 S. S4 AntG Promoter range2331522 1 24 62 annotation2331524 1 neomycin resistance gene (optimised) range2331524 1 129 922 annotation2331526 1 Nde1 site range2331526 1 126 131 BBa_K1041001_sequence 1 cgcgtgacgagcgtcaccacggggccgggggattgccgccgcctcctcgcgcctcttcctctgcgacaacacacgtgtgtggccgggaccggcggacggccgcggctgagtgaagaggagaaggccatatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactccaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z