BBa_K1041004 1 BBa_K1041004 AntG Promoter + Gus gene 2013-10-01T11:00:00Z 2015-05-08T01:08:51Z This part was designed by our team to aid our project in identifying new strains of Antimycin-Producing Actinomycetes.The 14 known biosynthetic gene clusters contain four operons: antAB, antCDE, antFG and antHIJKLMNO. The antA gene encodes a unique ECF RNA polymerase sigma factor, referred to as σAntA, which has the sole function of regulating antimycin synthesis by activating transcription of the antFG and antHIJKLMNO genes [1]. Homologues of the AntA sigma factor, the key regulatory protein in antimycin biosynthesis, are present in all known gene clusters [2]. Due to this property a biosensor was designed with the AntA-regulated promoter (antGp) controlling the expression of the reporter Gus. This part was produced by performing restriction digests of the part BBa_K1041001. Team NRP-UEA_Norwich 2013 desgined this part using biobricks BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and a gene encoding Gus ligated in front of the promoter of BBa_K1041001 to create a new biobrick. false false _1348_ 0 17128 9 Not in stock false Team NRP-UEA_Norwich 2013 desgined this part using biobricks BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and a gene encoding Gus ligated in front of the promoter of BBa_K1041001 to create a new biobrick. false Lucy Clark annotation2368987 1 rbs range2368987 1 113 121 annotation2368985 1 S. S4 AntG Promoter range2368985 1 24 62 annotation2368988 1 Nde site range2368988 1 126 131 annotation2368989 1 GUS gene range2368989 1 129 1940 BBa_K1041004_sequence 1 cgcgtgacgagcgtcaccacggggccgggggattgccgccgcctcctcgcgcctcttcctctgcgacaacacacgtgtgtggccgggaccggcggacggccgcggctgagtgaagaggagaaggccatatgctgcggcccgtcgaaaccccgacccgcgagatcaagaagctggacggcctgtgggcgttcagcctcgaccgggagaactgcggtatcgaccagcggtggtgggagagcgccctccaggagtcgcgcgccatcgccgtccccggcagcttcaacgaccagttcgcggacgcggacatccgcaactacgcggggaacgtctggtatcagcgggaggtgttcatccccaagggctgggccggtcagcgcatcgtgctgcgcttcgacgccgtgacccactacggcaaggtctgggtcaacaaccaggaagtgatggagcaccagggcggctacacgcccttcgaggcggacgtgaccccgtacgtcatcgccggcaagtcggtccgcatcacggtctgcgtcaacaacgagctcaactggcagaccatcccgcccggcatggtgatcacggacgagaacggcaagaagaagcagagctacttccacgacttcttcaactacgccggcatccaccgctcggtcatgctgtacacgacgccgaacacctgggtcgacgacatcacggtggtcacccacgtggcccaggactgcaaccacgcgtccgtggactggcaggtcgtcgccaacggcgacgtgagcgtggagctccgcgacgccgagcagcaggtcgtggcgaccgggcagggcacctcggggaccctccaggtcgtgaacccccacctctggcagccgggtgagggctacctgtacgagctgtgcgtgacggcgaagagccagaccgagtgcgacatctaccccctgcgcgtcggcatccggtccgtggcggtcaagggcgagcagttcctgatcaaccacaagccgttctacttcacgggcttcggtcggcacgaggacgccgacctccgcggcaagggcttcgacaacgtcctgatggtccacgaccacgccctgatggactggatcggcgccaactcgtaccggacctcgcactacccgtacgcggaagagatgctggactgggcggacgagcacgggatcgtcgtcatcgacgaaaccgccgcggtgggcttcaacctgtccctcggcatcggcttcgaggccggcaacaagccgaaagagctctactccgaagaggccgtcaacggcgaaacccagcaggcccacctccaggcgatcaaagagctcatcgcccgcgacaagaaccacccgtccgtcgtgatgtggtcgatcgccaacgagccggacacccggccgcagggtgcccgggagtacttcgccccgctggccgaggccacccggaagctcgaccccacccggcccatcacctgcgtgaacgtgatgttctgcgacgcccacacggacaccatctccgacctcttcgacgtcctgtgcctgaaccgctactacggctggtacgtgcagtccggggacctggaaacggccgagaaggtcctggagaaagagctgctggcgtggcaggagaagctgcaccagccgatcatcatcaccgagtacggcgtggacacgctggccggtctgcactccatgtacaccgacatgtggtccgaagagtaccagtgcgcgtggctggacatgtaccaccgcgtgttcgaccgcgtcagcgcggtcgtcggcgagcaggtgtggaacttcgccgacttcgccacctcccagggcatcctccgcgtcgggggcaacaagaagggcatcttcacccgggaccgcaagcccaagtcggcggcgttcctgctccagaagcggtggaccgggatgaacttcggggagaagccgcagcagggcgggaagcagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z