BBa_K1042015
type: ComponentDefinition
persistent identity: BBa K1042015
display id: BBa_K1042015
version: 1
was generated by: iGEM to SBOL conversion
description: Cadmium Responsive GFP+Ogr Activator
created: 2013-09-25T11:00:00Z
modified: 2015-05-08T01:08:51Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: After creating two intermediate parts, the upstream one being the Cadmium Promoter with Ogr Activator ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1042012 BBa_K1042012]) and the downstream one being the PO Promoter with GFP ([http://parts.igem.org/Part:BBa_K1042013 BBa_K1042013]), we digested the intermediates and ligated them to the linearized pSB1C3 plasmid provided. The resulting colonies were tested for responsive GFP production from the addition of cadmium.
discontinued: false
dominant: false
experience: experience/None
group u list: _1349_
m user id: 0
owner id: 9220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: test
star: false
creator: Parth Patel
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1042013
component: BBa_K1042012
sequence annotation: BBa_K1042012
sequence annotation: BBa_K1042013
sequence: https://synbiohub.org/public/igem/BBa_K1042015_sequence/1

BBa_K1042013
type: Component
persistent identity: component2363668
display id: component2363668
version: 1
top level: BBa_K1042015
definition: BBa_K1042013
access: public

BBa_K1042012
type: Component
persistent identity: component2363657
display id: component2363657
version: 1
top level: BBa_K1042015
definition: BBa_K1042012
access: public

BBa_K1042012
type: SequenceAnnotation
persistent identity: annotation2363657
display id: annotation2363657
version: 1
top level: BBa_K1042015
location: https://synbiohub.org/public/igem/BBa_K1042015/annotation2363657/range2363657/1
component: BBa_K1042012

https://synbiohub.org/public/igem/BBa_K1042015/annotation2363657/range2363657/1
type: Range
persistent identity: range2363657
display id: range2363657
version: 1
top level: BBa_K1042015
direction: direction/reverse
start: 1
end: 450
orientation: inline

BBa_K1042013
type: SequenceAnnotation
persistent identity: annotation2363668
display id: annotation2363668
version: 1
top level: BBa_K1042015
location: https://synbiohub.org/public/igem/BBa_K1042015/annotation2363668/range2363668/1
component: BBa_K1042013

https://synbiohub.org/public/igem/BBa_K1042015/annotation2363668/range2363668/1
type: Range
persistent identity: range2363668
display id: range2363668
version: 1
top level: BBa_K1042015
direction: direction/reverse
start: 459
end: 1413
orientation: inline

BBa_K1042012
type: ComponentDefinition
persistent identity: BBa K1042012
display id: BBa_K1042012
version: 1
was generated by: iGEM to SBOL conversion
description: Cadmium Promoter + P2 ogr activator
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:08:51Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: test
discontinued: false
dominant: false
experience: experience/None
group u list: _1349_
m user id: 0
owner id: 9218
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: test
star: false
creator: Parth Patel
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K174015
component: BBa_I746350
sequence annotation: BBa_K174015
sequence annotation: BBa_I746350
sequence: https://synbiohub.org/public/igem/BBa_K1042012_sequence/1

BBa_K174015
type: Component
persistent identity: component2363628
display id: component2363628
version: 1
top level: BBa_K1042012
definition: BBa_K174015
access: public

BBa_I746350
type: Component
persistent identity: component2363632
display id: component2363632
version: 1
top level: BBa_K1042012
definition: BBa_I746350
access: public

BBa_K174015
type: SequenceAnnotation
persistent identity: annotation2363628
display id: annotation2363628
version: 1
top level: BBa_K1042012
location: https://synbiohub.org/public/igem/BBa_K1042012/annotation2363628/range2363628/1
component: BBa_K174015

https://synbiohub.org/public/igem/BBa_K1042012/annotation2363628/range2363628/1
type: Range
persistent identity: range2363628
display id: range2363628
version: 1
top level: BBa_K1042012
direction: direction/reverse
start: 1
end: 205
orientation: inline

BBa_I746350
type: SequenceAnnotation
persistent identity: annotation2363632
display id: annotation2363632
version: 1
top level: BBa_K1042012
location: https://synbiohub.org/public/igem/BBa_K1042012/annotation2363632/range2363632/1
component: BBa_I746350

https://synbiohub.org/public/igem/BBa_K1042012/annotation2363632/range2363632/1
type: Range
persistent identity: range2363632
display id: range2363632
version: 1
top level: BBa_K1042012
direction: direction/reverse
start: 214
end: 450
orientation: inline

BBa_K1042013
type: ComponentDefinition
persistent identity: BBa K1042013
display id: BBa_K1042013
version: 1
was generated by: iGEM to SBOL conversion
description: PO promoter + GFP with Stops
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:08:51Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: test
discontinued: false
dominant: false
experience: experience/None
group u list: _1349_
m user id: 0
owner id: 9218
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: test
star: false
creator: Parth Patel
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I746361
component: BBa_I13401
sequence annotation: BBa_I746361
sequence annotation: BBa_I13401
sequence: https://synbiohub.org/public/igem/BBa_K1042013_sequence/1

BBa_I746361
type: Component
persistent identity: component2363634
display id: component2363634
version: 1
top level: BBa_K1042013
definition: BBa_I746361
access: public

BBa_I13401
type: Component
persistent identity: component2363642
display id: component2363642
version: 1
top level: BBa_K1042013
definition: BBa_I13401
access: public

BBa_I746361
type: SequenceAnnotation
persistent identity: annotation2363634
display id: annotation2363634
version: 1
top level: BBa_K1042013
location: https://synbiohub.org/public/igem/BBa_K1042013/annotation2363634/range2363634/1
component: BBa_I746361

https://synbiohub.org/public/igem/BBa_K1042013/annotation2363634/range2363634/1
type: Range
persistent identity: range2363634
display id: range2363634
version: 1
top level: BBa_K1042013
direction: direction/reverse
start: 1
end: 92
orientation: inline

BBa_I13401
type: SequenceAnnotation
persistent identity: annotation2363642
display id: annotation2363642
version: 1
top level: BBa_K1042013
location: https://synbiohub.org/public/igem/BBa_K1042013/annotation2363642/range2363642/1
component: BBa_I13401

https://synbiohub.org/public/igem/BBa_K1042013/annotation2363642/range2363642/1
type: Range
persistent identity: range2363642
display id: range2363642
version: 1
top level: BBa_K1042013
direction: direction/reverse
start: 99
end: 955
orientation: inline

BBa_I746350
type: ComponentDefinition
persistent identity: BBa I746350
display id: BBa_I746350
version: 1
was generated by: iGEM to SBOL conversion
description: ogr activator from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The ogr activator taken from P2 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: P2 ogr
sequence: https://synbiohub.org/public/igem/BBa_I746350_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943865
display id: annotation1943865
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943865/range1943865/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I746350/annotation1943865/range1943865/1
type: Range
persistent identity: range1943865
display id: range1943865
version: 1
top level: BBa_I746350
direction: direction/forward
start: 1
end: 12
orientation: inline

P2 ogr
type: SequenceAnnotation
persistent identity: annotation1943867
display id: annotation1943867
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943867/range1943867/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I746350/annotation1943867/range1943867/1
type: Range
persistent identity: range1943867
display id: range1943867
version: 1
top level: BBa_I746350
direction: direction/forward
start: 19
end: 237
orientation: inline

P2 ogr
type: SequenceAnnotation
persistent identity: annotation1943866
display id: annotation1943866
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943866/range1943866/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746350/annotation1943866/range1943866/1
type: Range
persistent identity: range1943866
display id: range1943866
version: 1
top level: BBa_I746350
direction: direction/forward
start: 19
end: 19
orientation: inline

BBa_I13401
type: ComponentDefinition
persistent identity: BBa I13401
display id: BBa_I13401
version: 1
was generated by: iGEM to SBOL conversion
description: GFP reporter for RHS of library test constructs
created: 2005-04-05T11:00:00Z
modified: 2015-08-31T04:07:33Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part will be suffixed to a promoter.RBS library. The purpose of the experiment is to make a first pass at a library-based construction step to identify efficiency issues and also to examine maxPOPS/RIPS level.
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part was built by Jen as an intermediate to their nuts and bolts parts.
star: true
creator: jasonk
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13401_sequence/1

BBa_E0040
type: Component
persistent identity: component1476713
display id: component1476713
version: 1
top level: BBa_I13401
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1476728
display id: component1476728
version: 1
top level: BBa_I13401
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1476718
display id: component1476718
version: 1
top level: BBa_I13401
definition: BBa_B0010
access: public

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1476713
display id: annotation1476713
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
type: Range
persistent identity: range1476713
display id: range1476713
version: 1
top level: BBa_I13401
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1476718
display id: annotation1476718
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
type: Range
persistent identity: range1476718
display id: range1476718
version: 1
top level: BBa_I13401
direction: direction/forward
start: 729
end: 808
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1476728
display id: annotation1476728
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
type: Range
persistent identity: range1476728
display id: range1476728
version: 1
top level: BBa_I13401
direction: direction/forward
start: 817
end: 857
orientation: inline

BBa_K174015
type: ComponentDefinition
persistent identity: BBa K174015
display id: BBa_K174015
version: 1
was generated by: iGEM to SBOL conversion
description: Cadmium Sensor
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:10:58Z
mutable provenance: CadA promoter which has the binding site for CzrA protein is combined with ArsR binding site to create this AND gate Cadmium sensor.
owned by: james
owned by: myers
mutable description: This Cadmium sensor has binding sites for ArsR and CzrA metal sensors. By placing them together, our device works as an AND gate and senses Cadmium when both proteins bind to Cadmium. When Cadmium is not present both proteins bind to their relevant sites on the promoter and represses the expression of downstream genes. When Cadmium present in the environment, it binds to these repressors which then fall of from where they are bound to on the promoter. Hence the promoter becomes free to express the downstream genes as an indication of Cadmium. This combinatorial approach enables us to filter out sensing other metals. ArsR and CzrA can bind to different metals, however when both are used as an ANd gate, they will only recognize Cadmium.
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 0
owner id: 3942
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We wanted to sense Cadmium accurately and used a combinatorial approach to achieve our goal.
star: false
creator: The Newcastle 2009 iGEM team
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K174016
component: BBa_K174017
sequence annotation: BBa_K174016
sequence annotation: BBa_K174017
sequence: https://synbiohub.org/public/igem/BBa_K174015_sequence/1

BBa_K174016
type: Component
persistent identity: component2040851
display id: component2040851
version: 1
top level: BBa_K174015
definition: BBa_K174016
access: public

BBa_K174017
type: Component
persistent identity: component2040856
display id: component2040856
version: 1
top level: BBa_K174015
definition: BBa_K174017
access: public

BBa_K174016
type: SequenceAnnotation
persistent identity: annotation2040851
display id: annotation2040851
version: 1
top level: BBa_K174015
location: https://synbiohub.org/public/igem/BBa_K174015/annotation2040851/range2040851/1
component: BBa_K174016

https://synbiohub.org/public/igem/BBa_K174015/annotation2040851/range2040851/1
type: Range
persistent identity: range2040851
display id: range2040851
version: 1
top level: BBa_K174015
direction: direction/reverse
start: 1
end: 26
orientation: inline

BBa_K174017
type: SequenceAnnotation
persistent identity: annotation2040856
display id: annotation2040856
version: 1
top level: BBa_K174015
location: https://synbiohub.org/public/igem/BBa_K174015/annotation2040856/range2040856/1
component: BBa_K174017

https://synbiohub.org/public/igem/BBa_K174015/annotation2040856/range2040856/1
type: Range
persistent identity: range2040856
display id: range2040856
version: 1
top level: BBa_K174015
direction: direction/reverse
start: 35
end: 205
orientation: inline

BBa_I746361
type: ComponentDefinition
persistent identity: BBa I746361
display id: BBa_I746361
version: 1
was generated by: iGEM to SBOL conversion
description: PO promoter from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The source of the DNA is the P2 phage genome.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PO
sequence: https://synbiohub.org/public/igem/BBa_I746361_sequence/1

PO
type: SequenceAnnotation
persistent identity: annotation1943784
display id: annotation1943784
version: 1
top level: BBa_I746361
location: https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
type: Range
persistent identity: range1943784
display id: range1943784
version: 1
top level: BBa_I746361
direction: direction/forward
start: 1
end: 92
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K174016
type: ComponentDefinition
persistent identity: BBa K174016
display id: BBa_K174016
version: 1
was generated by: iGEM to SBOL conversion
description: Promoterless ArsR binding site
created: 2009-10-13T11:00:00Z
modified: 2015-07-24T01:17:49Z
mutable provenance: ''B. subtilis''
owned by: james
owned by: myers
mutable description: This part purely holds the binding site for ArsR protein which can be released when bound to different metals. Hence this part can be combined with any promoter to sense metals that can bind to ArsR protein. When added in front of a promoter with another metal sensor's protein's binding site, it can form an AND gate to sensitively detect specific metals.
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 4206
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It was developed mainly to use combinatorial approaches for metal sensing.
star: false
creator: The Newcastle 2009 iGEM team
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ArsR binding site
sequence: https://synbiohub.org/public/igem/BBa_K174016_sequence/1

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation2040774
display id: annotation2040774
version: 1
top level: BBa_K174016
location: https://synbiohub.org/public/igem/BBa_K174016/annotation2040774/range2040774/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K174016/annotation2040774/range2040774/1
type: Range
persistent identity: range2040774
display id: range2040774
version: 1
top level: BBa_K174016
direction: direction/forward
start: 9
end: 26
orientation: inline

BBa_K174017
type: ComponentDefinition
persistent identity: BBa K174017
display id: BBa_K174017
version: 1
was generated by: iGEM to SBOL conversion
description: CadA promoter with CzrA binding site
created: 2009-10-13T11:00:00Z
modified: 2015-07-24T01:17:17Z
mutable provenance: It is taken from ''B. subtilis'' ''cadA'' promoter.
owned by: james
owned by: myers
mutable description: In ''B. subtilis'' CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by CzrA repressor protein. When certain metals get inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system. By taking the promoter of CadA system, we created a generic metal sensor. Hence when it is combined with coding sequences of other genes, it will regulate the expression of these genes upon sensing metals.
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 4206
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It was developed mainly to use combinatorial approaches for metal sensing
star: false
creator: The Newcastle 2009 iGEM team
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cadA promoter
sequence annotation: sigA -35
sequence annotation: sigA -10
sequence annotation: CzrA binding site
sequence: https://synbiohub.org/public/igem/BBa_K174017_sequence/1

cadA promoter
type: SequenceAnnotation
persistent identity: annotation2040827
display id: annotation2040827
version: 1
top level: BBa_K174017
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040827/range2040827/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K174017/annotation2040827/range2040827/1
type: Range
persistent identity: range2040827
display id: range2040827
version: 1
top level: BBa_K174017
direction: direction/forward
start: 11
end: 144
orientation: inline

sigA -35
type: SequenceAnnotation
persistent identity: annotation2040828
display id: annotation2040828
version: 1
top level: BBa_K174017
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040828/range2040828/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K174017/annotation2040828/range2040828/1
type: Range
persistent identity: range2040828
display id: range2040828
version: 1
top level: BBa_K174017
direction: direction/forward
start: 18
end: 23
orientation: inline

sigA -10
type: SequenceAnnotation
persistent identity: annotation2040829
display id: annotation2040829
version: 1
top level: BBa_K174017
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040829/range2040829/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K174017/annotation2040829/range2040829/1
type: Range
persistent identity: range2040829
display id: range2040829
version: 1
top level: BBa_K174017
direction: direction/forward
start: 42
end: 47
orientation: inline

CzrA binding site
type: SequenceAnnotation
persistent identity: annotation2040830
display id: annotation2040830
version: 1
top level: BBa_K174017
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040830/range2040830/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K174017/annotation2040830/range2040830/1
type: Range
persistent identity: range2040830
display id: range2040830
version: 1
top level: BBa_K174017
direction: direction/forward
start: 56
end: 86
orientation: inline

https://synbiohub.org/public/igem/BBa_K1042015_sequence/1
type: Sequence
persistent identity: BBa K1042015 sequence
display id: BBa_K1042015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtaatcaaaataaattgatttattttactagaggcgcttgctgtttttcattgacactttcttggaaaacaacatataataggtgtaacttatatatgagtatatgctcatatatataaaataaatacaatactcattgatacgctttgaagagggaaccctaaatcggaaggtaagctaagaggaggaactactatggctagctactagagaaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaatactagagcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1042012_sequence/1
type: Sequence
persistent identity: BBa K1042012 sequence
display id: BBa_K1042012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtaatcaaaataaattgatttattttactagaggcgcttgctgtttttcattgacactttcttggaaaacaacatataataggtgtaacttatatatgagtatatgctcatatatataaaataaatacaatactcattgatacgctttgaagagggaaccctaaatcggaaggtaagctaagaggaggaactactatggctagctactagagaaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1042013_sequence/1
type: Sequence
persistent identity: BBa K1042013 sequence
display id: BBa_K1042013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746350_sequence/1
type: Sequence
persistent identity: BBa I746350 sequence
display id: BBa_I746350_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13401_sequence/1
type: Sequence
persistent identity: BBa I13401 sequence
display id: BBa_I13401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K174015_sequence/1
type: Sequence
persistent identity: BBa K174015 sequence
display id: BBa_K174015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtaatcaaaataaattgatttattttactagaggcgcttgctgtttttcattgacactttcttggaaaacaacatataataggtgtaacttatatatgagtatatgctcatatatataaaataaatacaatactcattgatacgctttgaagagggaaccctaaatcggaaggtaagctaagaggaggaactactatggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746361_sequence/1
type: Sequence
persistent identity: BBa I746361 sequence
display id: BBa_I746361_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K174016_sequence/1
type: Sequence
persistent identity: BBa K174016 sequence
display id: BBa_K174016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtaatcaaaataaattgatttattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K174017_sequence/1
type: Sequence
persistent identity: BBa K174017 sequence
display id: BBa_K174017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgcttgctgtttttcattgacactttcttggaaaacaacatataataggtgtaacttatatatgagtatatgctcatatatataaaataaatacaatactcattgatacgctttgaagagggaaccctaaatcggaaggtaagctaagaggaggaactactatggctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z