BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1044007
1
BBa_K1044007
T7 promoter (strong promoter from T7 bacteriophage)+RBS (Elowitz 1999) -- defines RBS efficiency
2013-09-22T11:00:00Z
2015-05-08T01:08:51Z
BBa_I712074,BBa_B0034
T7 promoter (strong promoter from T7 bacteriophage)+RBS (Elowitz 1999) -- defines RBS efficiency
false
false
_1351_
0
16207
9
It's complicated
false
none
false
Koki Tsutsumi
component2358788
1
BBa_B0034
component2358786
1
BBa_I712074
annotation2358788
1
BBa_B0034
range2358788
1
55
66
annotation2358786
1
BBa_I712074
range2358786
1
1
46
BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1044007_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaa
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z