BBa_K1045003
1
DacA
Diadenylate cyclase domain of Listeria monocytogenes cdaA (DacA)
2013-08-21T11:00:00Z
2015-05-08T01:08:52Z
The part was amplified using pcr from L. monocytogenes genomic DNA.
Cyclic di-AMP is involved in cell signaling of gram positive bacteria including L. monocytogenes, B. subtilis, S. aureus, and S. pneumoniae.
The diadenylate cyclase domain of Listeria monocytogenes cdaA protein generates cyclic di-AMP for cell signaling. The part BBa_K1045003 described here covers the amino acids 100 to 273 of the full length coding sequence of cdaA.
false
false
_1352_
0
16038
9
It's complicated
true
The genomic sequence of the part BBa_K1045003 contained a SpeI restriction site on position 456 of the DNA sequence. The restriction site was deleted without altering the amino acid sequence of the protein.
false
iGEM Team G??ttingen 2013
annotation2360237
1
stop
range2360237
1
526
528
annotation2360236
1
Start
range2360236
1
1
3
annotation2360250
1
Diadenylate cyclase domain
range2360250
1
1
528
BBa_K1045003_sequence
1
atgtatggatcaagaattgagcgtgaacagcatcatttaatcgagtctatcgaaaaatccacccaatatatggcaaaacgtcgaattggggcactgatttcagtggcacgcgatacaggcatggacgattatattgaaacaggtattccgttaaatgcaaaaatttcttctcaattattaattaatatttttattccgaatacaccgcttcatgatggagcagttattattaaaggaaacgaaattgcatcggcagcaagttacttgccactttcagatagcccgttcttatccaaagaacttggaacgcgtcaccgggctgcacttgggattagtgaagtgacagatagtattacgattgtagtttctgaagagactggcggaatttccctaactaaaggtggagaacttttccgtgatgtgtctgaagaagagttacataaaattcttcttaaagaactggtcacagtaactgcaaagaaaccttctatcttttctaaatggaaaggaggcaaaagcgaatgatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z