BBa_K105017 1 BBa_K105017 Ura3 - Orotidine-5-phosphate decarboxylase 2008-10-21T11:00:00Z 2015-05-08T01:08:52Z Amplification from the plasmid pRS416 (Sikorski & Hieter) The Orotidine-5'-phosphate (OMP) decarboxylase catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines in ''S. cerevisiae''. It converts OMP into uridine monophosphate (UMP) and capable to transform 5-FOA into the toxic 5-fluorouracil. Under the name of Ura3 it is one of the commonly used auxotrophic markers in Yeast. There exists a number of ''S. cerevisiae'' strains which are deleated for this enzyme. <br\n> <br\n> Our BioBrick contains the protein coding sequence as well as the natural promoter and terminator of the gene. To avoid a transcription of genes downstream of this part, it might be usefull put a strong termintor (i.e. Adh1_term: [[Part:BBa_J63002| BBa_J63002]]) in between the parts. <br\n> false false _253_ 0 3313 9 It's complicated true Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design. So there is no base in between the BioBrick and the restriction sites. <br\n> The deletation of an internal Pst1 site has been possible with a silent mutation. true Michael Wild, Katja Karstens annotation1983855 1 Orotidine-5'-phosphate decarboxylase range1983855 1 232 1036 annotation1983856 1 please mutate T -> C range1983856 1 211 211 BBa_K105017_sequence 1 accacagcttttcaattcaattcatcattttttttttattcttttttttgatttcggtttctttgaaatttttttgattcggtaatctccgaacagaaggaagaacgaaggaaggagcacagacttagattggtatatatacgcatatgtagtgttgaagaaacatgaaattgcccagtattcttaacccaactgcacagaacaaaaacctgcaggaaacgaagataaatcatgtcgaaagctacatataaggaacgtgctgctactcatcctagtcctgttgctgccaagctatttaatatcatgcacgaaaagcaaacaaacttgtgtgcttcattggatgttcgtaccaccaaggaattactggagttagttgaagcattaggtcccaaaatttgtttactaaaaacacatgtggatatcttgactgatttttccatggagggcacagttaagccgctaaaggcattatccgccaagtacaattttttactcttcgaagacagaaaatttgctgacattggtaatacagtcaaattgcagtactctgcgggtgtatacagaatagcagaatgggcagacattacgaatgcacacggtgtggtgggcccaggtattgttagcggtttgaagcaggcggcagaagaagtaacaaaggaacctagaggccttttgatgttagcagaattgtcatgcaagggctccctatctactggagaatatactaagggtactgttgacattgcgaagagcgacaaagattttgttatcggctttattgctcaaagagacatgggtggaagagatgaaggttacgattggttgattatgacacccggtgtgggtttagatgacaagggagacgcattgggtcaacagtatagaaccgtggatgatgtggtctctacaggatctgacattattattgttggaagaggactatttgcaaagggaagggatgctaaggtagagggtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcggccagcaaaactaaaaaactgtattataagtaaatgcatgtatactaaactcacaaattagagcttcaatttaattatatcagttattaccctatgcggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z