BBa_K105030 1 BBa_K105030 cyc28 minimal promoter 2008-10-25T11:00:00Z 2015-05-08T01:08:52Z Amplification and Mutation from the plasmid pAH3 This is a derivate of [[Part:BBa_K105027| BBa_K105027]]. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 28 % of the natural yeast CYC1 promoter. <br> <br> false false _253_ 0 3313 9 It's complicated true This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!<br> <br> Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design. true Michael Wild annotation1989840 1 A -> T range1989840 1 22 22 annotation1989839 1 TATA-box range1989839 1 17 23 BBa_K105030_sequence 1 gcatgtgctctgtatgtatattaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z