BBa_K1051300 1 BBa_K1051300 cln2 promoter (G1 phase to S phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z ATTTGTCTCAACGGAAGTACACGAAATTCGTCCCGCTGAACCTCAAAACTGCGTGTTCTA GTCACGAAACGCGCCAAAAACCGTTATGTTTCCGCATGCGGATACCTAGCGAAGAGCACA TTTGGCGTAGCCTGTTAAGTATAACTGCTAAAAATTGAAATGAACACCAACTGAAACAAC TGTTGGCCCTTTTTGAGGATCTAACCTGCGAAATGTTGATCTGACAGGCCTACTGAAAAA CGGAAAGGAAAAAAGGCCTGCGCAAACGCAGAACGCAGATCCGCCTTCTATTTGCGTATC GCACGCAGCCTCTGGCTACTTGTTTAACTTACTATCTCTTTCATATATAAAAAAATAGCA ATCGACTCTGGTAACTATTATGCTCCTCTTACTGGTTTTTTAGTGAATTGGCAATAGATA ATATCAAGGACAACTTACTTTTATTTTAACATCTACTTTACTTCATCGTCAAATTTTCTT CTTATATACAGCATTGCTAGACTCTATAGCTGCCAATTCATTCGCTTACCACATCATAAT TTGCATACAAAAGAAAATCTTTTCTTTTTTCCATTCATTCATTAA ATTTA ACGACAGACA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 It's complicated false We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATTTGTCTCAACGGAAGTAC downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTCTGTCGTTAAATTTAAT false Kequan Lin BBa_K1051300_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z