BBa_K1051304 1 BBa_K1051304 cln3 promoter (M phase to G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z GAAAAATTATCAGGCAAGAAAAAGAAATTACCAAGCCTGCTCTCACTGTAATGATCAAGT TACATAAATTTACTATCGGATTAGTGTGTCCCTCTGCCACATTTCCATATTTGGCCTTGG GTTTTTGCCCTCATCTTTTTTTTTTCTTCCTCCTACCTATTTATAATTTGTATATCTGTA CTTTCCTCGAGCTTTTAATCTTCTTCTTAAACATTTTCTTGTGTAGTATACTTTCACACA ATTTCTTTCTTGATTTTTTCTTCTACTACTGAGTCTGCCAGTCAAATGGATTTCTGAGGA AAGAGGACTATACCCATTAGGAAACGAATTGCCCGAGTAGTCTCCTCTGCCGACTTAAAC CAACCTTTTTCTATTTCTCTTTTCTTTTCTCCCTCTTTTTTCTCTGTACTAGCATCCAAA AGCAAGCATCCATCCGAGTCCCAGTCGCAATCTCACATCTCCAATTTAACGTATCCATTG CATTTCCTCATTCGGTTTAACTCCTCTGCATTTCTTTTCTGACCCATAGCATTTCTTACA TTCCATTGCATCTCCCTTTTACTCTCGTTCAAGACACTGATTTGATACGCTTTCTGTACG It is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 In stock false We get the sequence which is actived by cln3 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream: downstream: false Kequan Lin BBa_K1051304_sequence 1 cacctttatcgtgtgccacggcggtaaaaaactaccttcaggggtacgatacattctgtgctggcgaccacggggctgacagagacacccgtagaggctacattactgatttgggaaatttcccaaattggaaatatcacctcgtcgacgtgctgcggtgcatggctgttttacccgtttaggaaaaaactcggcgggttttcttgacgggcaaatgtcgccattcgtctcgtttgaacgcttggcctgtaaaacagctaactcattcactatctctatcctgcgaccgtgatgataccgaaagtatgctgatacagatcggtccctcatatcgcgatagggctttctgagctctcctcccccttcttccctccccagccaaagaggccggttttccttctggggaagtgtcgcaaccaaacgctgtgctcaaaccctaaattgtgaactttcaagaaaaaaaaaaagaaaaagtgaaaaattatcaggcaagaaaaagaaattaccaagcctgctctcactgtaatgatcaagttacataaatttactatcggattagtgtgtccctctgccacatttccatatttggccttgggtttttgccctcatcttttttttttcttcctcctacctatttataatttgtatatctgtactttcctcgagcttttaatcttcttcttaaacattttcttgtgtagtatactttcacacaatttctttcttgattttttcttctactactgagtctgccagtcaaatggatttctgaggaaagaggactatacccattaggaaacgaattgcccgagtagtctcctctgccgacttaaaccaacctttttctatttctcttttcttttctccctcttttttctctgtactagcatccaaaagcaagcatccatccgagtcccagtcgcaatctcacatctccaatttaacgtatccattgcatttcctcattcggtttaactcctctgcatttcttttctgacccatagcatttcttacattccattgcatctcccttttactctcgttcaagacactgatttgatacgctttctgtacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z