BBa_K1051300 1 BBa_K1051300 cln2 promoter (G1 phase to S phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z ATTTGTCTCAACGGAAGTACACGAAATTCGTCCCGCTGAACCTCAAAACTGCGTGTTCTA GTCACGAAACGCGCCAAAAACCGTTATGTTTCCGCATGCGGATACCTAGCGAAGAGCACA TTTGGCGTAGCCTGTTAAGTATAACTGCTAAAAATTGAAATGAACACCAACTGAAACAAC TGTTGGCCCTTTTTGAGGATCTAACCTGCGAAATGTTGATCTGACAGGCCTACTGAAAAA CGGAAAGGAAAAAAGGCCTGCGCAAACGCAGAACGCAGATCCGCCTTCTATTTGCGTATC GCACGCAGCCTCTGGCTACTTGTTTAACTTACTATCTCTTTCATATATAAAAAAATAGCA ATCGACTCTGGTAACTATTATGCTCCTCTTACTGGTTTTTTAGTGAATTGGCAATAGATA ATATCAAGGACAACTTACTTTTATTTTAACATCTACTTTACTTCATCGTCAAATTTTCTT CTTATATACAGCATTGCTAGACTCTATAGCTGCCAATTCATTCGCTTACCACATCATAAT TTGCATACAAAAGAAAATCTTTTCTTTTTTCCATTCATTCATTAA ATTTA ACGACAGACA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 It's complicated false We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATTTGTCTCAACGGAAGTAC downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTCTGTCGTTAAATTTAAT false Kequan Lin BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051350 1 BBa_K1051350 K1051300 (cln2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator) 2013-07-17T11:00:00Z 2015-05-08T01:08:54Z . K1051300 (cln2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator) false false _1358_ 0 11943 9 Not in stock false . false Kang KANG component2378362 1 BBa_K1051000 component2378361 1 BBa_K1051300 component2378363 1 BBa_K1051008 annotation2378363 1 BBa_K1051008 range2378363 1 1690 1707 annotation2378362 1 BBa_K1051000 range2378362 1 1007 1681 annotation2378361 1 BBa_K1051300 range2378361 1 1 1000 BBa_K1051000 1 BBa_K1051000 Stop codon free RFP in RFC[23] standard 2013-07-01T11:00:00Z 2015-05-08T01:08:52Z engineered mutant of red fluorescent protein from Discosoma striata Red fluorescent protein with assembly standard 23 is engineered mutant of red fluorescent protein from Discosoma striata (coral). false false _1358_ 0 15912 9 It's complicated false You have to remove the termination codon before you use it false Xiang LI BBa_K1051350_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagacatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttactagagtaatttttattcattttt BBa_K1051300_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagaca BBa_K1051008_sequence 1 taatttttattcattttt BBa_K1051000_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z