BBa_K1051053 1 BBa_K1051053 K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator) 2013-07-22T11:00:00Z 2015-05-08T01:08:53Z Saccharomyces cerevisiae genomics sequence Yeast terminator TBY-1 joint with red flourescent protein. After added with 23 suffix and preffix, this composite biobricks can be joint with other coding gene sequence in our project. Thus it would be a reporter showing red flourescent,also as a terminator which can stop the transcription. false false _1358_ 0 15923 9 Not in stock false No false Jinchun Zhang component2331041 1 BBa_K1051006 component2331040 1 BBa_K1051001 annotation2331040 1 BBa_K1051001 range2331040 1 1 716 annotation2331041 1 BBa_K1051006 range2331041 1 725 755 BBa_K1051351 1 BBa_K1051351 K1051301 (clb2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator) 2013-07-17T11:00:00Z 2015-05-08T01:08:54Z . K1051301 (clb2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator) false false _1358_ 0 11943 9 Not in stock false . false Kang KANG component2331115 1 BBa_K1051053 component2331112 1 BBa_K1051301 annotation2331115 1 BBa_K1051053 range2331115 1 1070 1824 annotation2331112 1 BBa_K1051301 range2331112 1 1 1063 BBa_K1051001 1 BBa_K1051001 Stop codon free ECFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:52Z kit ECFP without stop coding false false _1358_ 0 15912 9 Not in stock false add prefix and suffix del stop coding false Xiang LI BBa_K1051006 1 BBa_K1051006 Saccharomycescerevisiae TBY-1 terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z Saccharomycescerevisiae Saccharomycescerevisiae terminator with stopcodon false false _1358_ 0 15912 9 It's complicated false stopcodon + terminator false Xiang LI BBa_K1051301 1 BBa_K1051301 clb2 promoter (during G2 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z CTAGC ATCTC ATAGC ATGGG CATAGAGTTTCAGAAATTTTGCTCTTAATGGAAAATATAA CCTCTTTGGGGAAAAGAGAAATAGATCCTTTCTCAAAAATTTAGAAAGGAAAAAAAATCA AAATATAATAAAATAGATTTTTTTTAGGTTAATTTTTTGCCAATTTAGCTAGACTTTTGA AAAGATCCAAAAATTTACTAAAATTGAATCTTTCTGGTATTAATTTTGTCCTATACCTCA AGAAGTAAAGTATTCTCGAATTGATACTTCTGAAAAGCGAAAGAAATATAGCTAAAGGAT ACTGGTTATTTATTCTTTCTTTTTTTCTTTCTTTTTGATTGAGCCAGTGTATAATAAAAA CCTACTGCGAGCTATTCAATAAATTGACATAGAATATCTGTTGAAGCGGTTCTTTGATTG AGCATCTGTTTTCCTTTTGTATTTTTGTCCTTTTAGATTCTTTTAACGACATTGGAGAAA TTTTAATCATTGTATAAAAACTTCATTCGCTCGTTTGTCAGAAGTTTTTTTATAACTTGC TATTTCCTTCCAAGAAGCCTTTTATTGATTACCCCCTCTCTCTCTTCATTGATCTTATAG It is a promoter which promotes transciption in G2 of the yeast cell cycle .If you want to express your proteins in G2 of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 Not in stock false We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTAGCATCTCATAGCATGGG downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTACTATAAGATCAATGAAGAGA false Kequan Lin BBa_K1051006_sequence 1 taatagtgtatgtgtataatatcatagttta BBa_K1051001_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaa BBa_K1051301_sequence 1 ttttgttatccctgttgttgttgttttataagtcctaatctcgcttgaatatcgcccatacttaccctgttcttgaccgtctactaaggtgccttagggggactcaaacttaagatcgccttctacgcgttttatgctatgatcattagtgttgatgcatcggaagtttaaaaattaagtgcgagtgcattagcacagtggaattattagaatgaccactactccttctaatcaaacacgcggaaatagccgccaaaagacagattttattccaaatgcgggtaactatttgtataatatgtttacatattgagcccgtttaggaaagtgcaagttcaaggcactaatcaaaaaaggagatttgtaaatatagcgaccgaatcaggaaaaggtcaacaacgaagttcgcgatatggatgaacttcggtgcctgtccctcgaggaaatagcaaactcgttacagctagcatctcatagcatgggcatagagtttcagaaattttgctcttaatggaaaatataacctctttggggaaaagagaaatagatcctttctcaaaaatttagaaaggaaaaaaaatcaaaatataataaaatagattttttttaggttaattttttgccaatttagctagacttttgaaaagatccaaaaatttactaaaattgaatctttctggtattaattttgtcctatacctcaagaagtaaagtattctcgaattgatacttctgaaaagcgaaagaaatatagctaaaggatactggttatttattctttctttttttctttctttttgattgagccagtgtataataaaaacctactgcgagctattcaataaattgacatagaatatctgttgaagcggttctttgattgagcatctgttttccttttgtatttttgtccttttagattcttttaacgacattggagaaattttaatcattgtataaaaacttcattcgctcgtttgtcagaagtttttttataacttgctatttccttccaagaagccttttattgattaccccctctctctcttcattgatcttatag BBa_K1051053_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaatactagagtaatagtgtatgtgtataatatcatagttta BBa_K1051351_sequence 1 ttttgttatccctgttgttgttgttttataagtcctaatctcgcttgaatatcgcccatacttaccctgttcttgaccgtctactaaggtgccttagggggactcaaacttaagatcgccttctacgcgttttatgctatgatcattagtgttgatgcatcggaagtttaaaaattaagtgcgagtgcattagcacagtggaattattagaatgaccactactccttctaatcaaacacgcggaaatagccgccaaaagacagattttattccaaatgcgggtaactatttgtataatatgtttacatattgagcccgtttaggaaagtgcaagttcaaggcactaatcaaaaaaggagatttgtaaatatagcgaccgaatcaggaaaaggtcaacaacgaagttcgcgatatggatgaacttcggtgcctgtccctcgaggaaatagcaaactcgttacagctagcatctcatagcatgggcatagagtttcagaaattttgctcttaatggaaaatataacctctttggggaaaagagaaatagatcctttctcaaaaatttagaaaggaaaaaaaatcaaaatataataaaatagattttttttaggttaattttttgccaatttagctagacttttgaaaagatccaaaaatttactaaaattgaatctttctggtattaattttgtcctatacctcaagaagtaaagtattctcgaattgatacttctgaaaagcgaaagaaatatagctaaaggatactggttatttattctttctttttttctttctttttgattgagccagtgtataataaaaacctactgcgagctattcaataaattgacatagaatatctgttgaagcggttctttgattgagcatctgttttccttttgtatttttgtccttttagattcttttaacgacattggagaaattttaatcattgtataaaaacttcattcgctcgtttgtcagaagtttttttataacttgctatttccttccaagaagccttttattgattaccccctctctctcttcattgatcttatagtactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaatactagagtaatagtgtatgtgtataatatcatagttta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z