BBa_K1051352 1 BBa_K1051352 K1051302 (clb5 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator) 2013-07-17T11:00:00Z 2015-05-08T01:08:54Z . K1051302 (clb5 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator) false false _1358_ 0 11943 9 Not in stock false . false Kang KANG component2378365 1 BBa_K1051000 component2378364 1 BBa_K1051302 component2378366 1 BBa_K1051008 annotation2378365 1 BBa_K1051000 range2378365 1 655 1329 annotation2378364 1 BBa_K1051302 range2378364 1 1 648 annotation2378366 1 BBa_K1051008 range2378366 1 1338 1355 BBa_K1051302 1 BBa_K1051302 clb5 promoter (during G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z CGATCCTGAGTGCGAGCTTTCTTTTTTAGACTACTAATTTTATTTTGCTAGTCATTTTTT TTTTATACTCAAAAAGTAAAAAGACTACGAGTATATTCAAAGTAAAAAACGAACGTCAAA CTATCTCGATTAAAACTTGTCATACTGTGGGTATCATATTCTGTGCCCTCAGTGAAAAGA ACCAGCAAAAGAACGCGCATCTCGAGTGAAGACGCGCCCTTGATGGTACAAAATTTAACG GGAAGGCGCGTCGTGATGTTCACGCGCTTTGCCCACATTGGGATAGCGCCCACAGCATAT CTGTGCTAAACTCACTTTTCCTAGTGACTGCCGATAGCTACTGCCATCTACCGCGAAGGG AACTTCATTTGCGTTCATCGGTTTATTAGAAGCTACTTGGAACTAATTCTTAAGCTTCTC AAGAAAAGTTTTTTTTCTGTCTATCTATTGAAGTCTTTTTGTCTTTGTACTTCAAGAGAC TCAATCACCTAAAGCTTTTCACGGCCAATTAGTTGTCTCACACAAAGCAAAATAAGCTTA ATAATTAGCAGTAACGCGCTTTTCCCTGTATTTAAAGCCGCTGAACACCTTTACTGAACA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb5 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 Not in stock false We get the sequence which is actived by clb5 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATCCTGAGTGCGAGCTTT downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTTCAGTAAAGGTGTTCAG false Kequan Lin BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051000 1 BBa_K1051000 Stop codon free RFP in RFC[23] standard 2013-07-01T11:00:00Z 2015-05-08T01:08:52Z engineered mutant of red fluorescent protein from Discosoma striata Red fluorescent protein with assembly standard 23 is engineered mutant of red fluorescent protein from Discosoma striata (coral). false false _1358_ 0 15912 9 It's complicated false You have to remove the termination codon before you use it false Xiang LI BBa_K1051008_sequence 1 taatttttattcattttt BBa_K1051000_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgct BBa_K1051302_sequence 1 atggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttcttttttagactactaattttattttgctagtcattttttttttatactcaaaaagtaaaaagactacgagtatattcaaagtaaaaaacgaacgtcaaactatctcgattaaaacttgtcatactgtgggtatcatattctgtgccctcagtgaaaagaaccagcaaaagaacgcgcatctcgagtgaagacgcgcccttgatggtacaaaatttaacgggaaggcgcgtcgtgatgttcacgcgctttgcccacattgggatagcgcccacagcatatctgtgctaaactcacttttcctagtgactgccgatagctactgccatctaccgcgaagggaacttcatttgcgttcatcggtttattagaagctacttggaactaattcttaagcttctcaagaaaagttttttttctgtctatctattgaagtctttttgtctttgtacttcaagagactcaatcacctaaagcttttcacggccaattagttgtctcacacaaagcaaaataagcttaataattagcagtaacgcgcttttccctgtatttaaagccgctgaacacctttactgaaca BBa_K1051352_sequence 1 atggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttcttttttagactactaattttattttgctagtcattttttttttatactcaaaaagtaaaaagactacgagtatattcaaagtaaaaaacgaacgtcaaactatctcgattaaaacttgtcatactgtgggtatcatattctgtgccctcagtgaaaagaaccagcaaaagaacgcgcatctcgagtgaagacgcgcccttgatggtacaaaatttaacgggaaggcgcgtcgtgatgttcacgcgctttgcccacattgggatagcgcccacagcatatctgtgctaaactcacttttcctagtgactgccgatagctactgccatctaccgcgaagggaacttcatttgcgttcatcggtttattagaagctacttggaactaattcttaagcttctcaagaaaagttttttttctgtctatctattgaagtctttttgtctttgtacttcaagagactcaatcacctaaagcttttcacggccaattagttgtctcacacaaagcaaaataagcttaataattagcagtaacgcgcttttccctgtatttaaagccgctgaacacctttactgaacatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttactagagtaatttttattcattttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z