BBa_K1051304 1 BBa_K1051304 cln3 promoter (M phase to G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z GAAAAATTATCAGGCAAGAAAAAGAAATTACCAAGCCTGCTCTCACTGTAATGATCAAGT TACATAAATTTACTATCGGATTAGTGTGTCCCTCTGCCACATTTCCATATTTGGCCTTGG GTTTTTGCCCTCATCTTTTTTTTTTCTTCCTCCTACCTATTTATAATTTGTATATCTGTA CTTTCCTCGAGCTTTTAATCTTCTTCTTAAACATTTTCTTGTGTAGTATACTTTCACACA ATTTCTTTCTTGATTTTTTCTTCTACTACTGAGTCTGCCAGTCAAATGGATTTCTGAGGA AAGAGGACTATACCCATTAGGAAACGAATTGCCCGAGTAGTCTCCTCTGCCGACTTAAAC CAACCTTTTTCTATTTCTCTTTTCTTTTCTCCCTCTTTTTTCTCTGTACTAGCATCCAAA AGCAAGCATCCATCCGAGTCCCAGTCGCAATCTCACATCTCCAATTTAACGTATCCATTG CATTTCCTCATTCGGTTTAACTCCTCTGCATTTCTTTTCTGACCCATAGCATTTCTTACA TTCCATTGCATCTCCCTTTTACTCTCGTTCAAGACACTGATTTGATACGCTTTCTGTACG It is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 In stock false We get the sequence which is actived by cln3 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream: downstream: false Kequan Lin BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051354 1 BBa_K1051354 K1051304 (cln3 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator) 2013-07-17T11:00:00Z 2015-05-08T01:08:54Z . K1051304 (cln3 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator) false false _1358_ 0 11943 9 Not in stock false . false Kang KANG component2378370 1 BBa_K1051304 component2378371 1 BBa_K1051000 component2378372 1 BBa_K1051008 annotation2378371 1 BBa_K1051000 range2378371 1 1082 1756 annotation2378372 1 BBa_K1051008 range2378372 1 1765 1782 annotation2378370 1 BBa_K1051304 range2378370 1 1 1075 BBa_K1051000 1 BBa_K1051000 Stop codon free RFP in RFC[23] standard 2013-07-01T11:00:00Z 2015-05-08T01:08:52Z engineered mutant of red fluorescent protein from Discosoma striata Red fluorescent protein with assembly standard 23 is engineered mutant of red fluorescent protein from Discosoma striata (coral). false false _1358_ 0 15912 9 It's complicated false You have to remove the termination codon before you use it false Xiang LI BBa_K1051354_sequence 1 cacctttatcgtgtgccacggcggtaaaaaactaccttcaggggtacgatacattctgtgctggcgaccacggggctgacagagacacccgtagaggctacattactgatttgggaaatttcccaaattggaaatatcacctcgtcgacgtgctgcggtgcatggctgttttacccgtttaggaaaaaactcggcgggttttcttgacgggcaaatgtcgccattcgtctcgtttgaacgcttggcctgtaaaacagctaactcattcactatctctatcctgcgaccgtgatgataccgaaagtatgctgatacagatcggtccctcatatcgcgatagggctttctgagctctcctcccccttcttccctccccagccaaagaggccggttttccttctggggaagtgtcgcaaccaaacgctgtgctcaaaccctaaattgtgaactttcaagaaaaaaaaaaagaaaaagtgaaaaattatcaggcaagaaaaagaaattaccaagcctgctctcactgtaatgatcaagttacataaatttactatcggattagtgtgtccctctgccacatttccatatttggccttgggtttttgccctcatcttttttttttcttcctcctacctatttataatttgtatatctgtactttcctcgagcttttaatcttcttcttaaacattttcttgtgtagtatactttcacacaatttctttcttgattttttcttctactactgagtctgccagtcaaatggatttctgaggaaagaggactatacccattaggaaacgaattgcccgagtagtctcctctgccgacttaaaccaacctttttctatttctcttttcttttctccctcttttttctctgtactagcatccaaaagcaagcatccatccgagtcccagtcgcaatctcacatctccaatttaacgtatccattgcatttcctcattcggtttaactcctctgcatttcttttctgacccatagcatttcttacattccattgcatctcccttttactctcgttcaagacactgatttgatacgctttctgtacgtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttactagagtaatttttattcattttt BBa_K1051008_sequence 1 taatttttattcattttt BBa_K1051304_sequence 1 cacctttatcgtgtgccacggcggtaaaaaactaccttcaggggtacgatacattctgtgctggcgaccacggggctgacagagacacccgtagaggctacattactgatttgggaaatttcccaaattggaaatatcacctcgtcgacgtgctgcggtgcatggctgttttacccgtttaggaaaaaactcggcgggttttcttgacgggcaaatgtcgccattcgtctcgtttgaacgcttggcctgtaaaacagctaactcattcactatctctatcctgcgaccgtgatgataccgaaagtatgctgatacagatcggtccctcatatcgcgatagggctttctgagctctcctcccccttcttccctccccagccaaagaggccggttttccttctggggaagtgtcgcaaccaaacgctgtgctcaaaccctaaattgtgaactttcaagaaaaaaaaaaagaaaaagtgaaaaattatcaggcaagaaaaagaaattaccaagcctgctctcactgtaatgatcaagttacataaatttactatcggattagtgtgtccctctgccacatttccatatttggccttgggtttttgccctcatcttttttttttcttcctcctacctatttataatttgtatatctgtactttcctcgagcttttaatcttcttcttaaacattttcttgtgtagtatactttcacacaatttctttcttgattttttcttctactactgagtctgccagtcaaatggatttctgaggaaagaggactatacccattaggaaacgaattgcccgagtagtctcctctgccgacttaaaccaacctttttctatttctcttttcttttctccctcttttttctctgtactagcatccaaaagcaagcatccatccgagtcccagtcgcaatctcacatctccaatttaacgtatccattgcatttcctcattcggtttaactcctctgcatttcttttctgacccatagcatttcttacattccattgcatctcccttttactctcgttcaagacactgatttgatacgctttctgtacg BBa_K1051000_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z