BBa_K1051300 1 BBa_K1051300 cln2 promoter (G1 phase to S phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z ATTTGTCTCAACGGAAGTACACGAAATTCGTCCCGCTGAACCTCAAAACTGCGTGTTCTA GTCACGAAACGCGCCAAAAACCGTTATGTTTCCGCATGCGGATACCTAGCGAAGAGCACA TTTGGCGTAGCCTGTTAAGTATAACTGCTAAAAATTGAAATGAACACCAACTGAAACAAC TGTTGGCCCTTTTTGAGGATCTAACCTGCGAAATGTTGATCTGACAGGCCTACTGAAAAA CGGAAAGGAAAAAAGGCCTGCGCAAACGCAGAACGCAGATCCGCCTTCTATTTGCGTATC GCACGCAGCCTCTGGCTACTTGTTTAACTTACTATCTCTTTCATATATAAAAAAATAGCA ATCGACTCTGGTAACTATTATGCTCCTCTTACTGGTTTTTTAGTGAATTGGCAATAGATA ATATCAAGGACAACTTACTTTTATTTTAACATCTACTTTACTTCATCGTCAAATTTTCTT CTTATATACAGCATTGCTAGACTCTATAGCTGCCAATTCATTCGCTTACCACATCATAAT TTGCATACAAAAGAAAATCTTTTCTTTTTTCCATTCATTCATTAA ATTTA ACGACAGACA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 It's complicated false We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATTTGTCTCAACGGAAGTAC downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTCTGTCGTTAAATTTAAT false Kequan Lin BBa_K1051053 1 BBa_K1051053 K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator) 2013-07-22T11:00:00Z 2015-05-08T01:08:53Z Saccharomyces cerevisiae genomics sequence Yeast terminator TBY-1 joint with red flourescent protein. After added with 23 suffix and preffix, this composite biobricks can be joint with other coding gene sequence in our project. Thus it would be a reporter showing red flourescent,also as a terminator which can stop the transcription. false false _1358_ 0 15923 9 Not in stock false No false Jinchun Zhang component2331040 1 BBa_K1051001 component2331041 1 BBa_K1051006 annotation2331041 1 BBa_K1051006 range2331041 1 725 755 annotation2331040 1 BBa_K1051001 range2331040 1 1 716 BBa_K1051001 1 BBa_K1051001 Stop codon free ECFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:52Z kit ECFP without stop coding false false _1358_ 0 15912 9 Not in stock false add prefix and suffix del stop coding false Xiang LI BBa_K1051355 1 BBa_K1051355 K1051300 (cln2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 ter 2013-07-18T11:00:00Z 2015-05-08T01:08:54Z 1334bp promoter+ECFP+stop codon false false _1358_ 0 15940 9 Not in stock false biobrick false Kequan Lin component2331111 1 BBa_K1051053 component2331108 1 BBa_K1051300 annotation2331108 1 BBa_K1051300 range2331108 1 1 1000 annotation2331111 1 BBa_K1051053 range2331111 1 1007 1761 BBa_K1051006 1 BBa_K1051006 Saccharomycescerevisiae TBY-1 terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z Saccharomycescerevisiae Saccharomycescerevisiae terminator with stopcodon false false _1358_ 0 15912 9 It's complicated false stopcodon + terminator false Xiang LI BBa_K1051006_sequence 1 taatagtgtatgtgtataatatcatagttta BBa_K1051001_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaa BBa_K1051355_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagacatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaatactagagtaatagtgtatgtgtataatatcatagttta BBa_K1051300_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagaca BBa_K1051053_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaatactagagtaatagtgtatgtgtataatatcatagttta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z