BBa_K1051300 1 BBa_K1051300 cln2 promoter (G1 phase to S phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z ATTTGTCTCAACGGAAGTACACGAAATTCGTCCCGCTGAACCTCAAAACTGCGTGTTCTA GTCACGAAACGCGCCAAAAACCGTTATGTTTCCGCATGCGGATACCTAGCGAAGAGCACA TTTGGCGTAGCCTGTTAAGTATAACTGCTAAAAATTGAAATGAACACCAACTGAAACAAC TGTTGGCCCTTTTTGAGGATCTAACCTGCGAAATGTTGATCTGACAGGCCTACTGAAAAA CGGAAAGGAAAAAAGGCCTGCGCAAACGCAGAACGCAGATCCGCCTTCTATTTGCGTATC GCACGCAGCCTCTGGCTACTTGTTTAACTTACTATCTCTTTCATATATAAAAAAATAGCA ATCGACTCTGGTAACTATTATGCTCCTCTTACTGGTTTTTTAGTGAATTGGCAATAGATA ATATCAAGGACAACTTACTTTTATTTTAACATCTACTTTACTTCATCGTCAAATTTTCTT CTTATATACAGCATTGCTAGACTCTATAGCTGCCAATTCATTCGCTTACCACATCATAAT TTGCATACAAAAGAAAATCTTTTCTTTTTTCCATTCATTCATTAA ATTTA ACGACAGACA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 It's complicated false We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATTTGTCTCAACGGAAGTAC downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTCTGTCGTTAAATTTAAT false Kequan Lin BBa_K1051002 1 BBa_K1051002 Stop codon free EYFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit EYFP without stop coding false false _1358_ 0 15912 9 Not in stock false add prefix and suffix del stop coding false Xiang LI BBa_K1051360 1 BBa_K1051360 K1051300(cln2 promoter) + K1051002 (non stop codon EYFP) + K1051008 (Stop codon + CAA terminator) 2013-07-18T11:00:00Z 2015-05-08T01:08:54Z 1335bp cln2 promoter+EYFP+stop codon false false _1358_ 0 15940 9 Not in stock false biobrick false Kequan Lin component2378374 1 BBa_K1051002 component2378375 1 BBa_K1051008 component2378373 1 BBa_K1051300 annotation2378374 1 BBa_K1051002 range2378374 1 1007 1723 annotation2378373 1 BBa_K1051300 range2378373 1 1 1000 annotation2378375 1 BBa_K1051008 range2378375 1 1732 1749 BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051360_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagacatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtactagagtaatttttattcattttt BBa_K1051002_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag BBa_K1051300_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagaca BBa_K1051008_sequence 1 taatttttattcattttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z