BBa_K1051002 1 BBa_K1051002 Stop codon free EYFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit EYFP without stop coding false false _1358_ 0 15912 9 Not in stock false add prefix and suffix del stop coding false Xiang LI BBa_K1051302 1 BBa_K1051302 clb5 promoter (during G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z CGATCCTGAGTGCGAGCTTTCTTTTTTAGACTACTAATTTTATTTTGCTAGTCATTTTTT TTTTATACTCAAAAAGTAAAAAGACTACGAGTATATTCAAAGTAAAAAACGAACGTCAAA CTATCTCGATTAAAACTTGTCATACTGTGGGTATCATATTCTGTGCCCTCAGTGAAAAGA ACCAGCAAAAGAACGCGCATCTCGAGTGAAGACGCGCCCTTGATGGTACAAAATTTAACG GGAAGGCGCGTCGTGATGTTCACGCGCTTTGCCCACATTGGGATAGCGCCCACAGCATAT CTGTGCTAAACTCACTTTTCCTAGTGACTGCCGATAGCTACTGCCATCTACCGCGAAGGG AACTTCATTTGCGTTCATCGGTTTATTAGAAGCTACTTGGAACTAATTCTTAAGCTTCTC AAGAAAAGTTTTTTTTCTGTCTATCTATTGAAGTCTTTTTGTCTTTGTACTTCAAGAGAC TCAATCACCTAAAGCTTTTCACGGCCAATTAGTTGTCTCACACAAAGCAAAATAAGCTTA ATAATTAGCAGTAACGCGCTTTTCCCTGTATTTAAAGCCGCTGAACACCTTTACTGAACA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb5 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 Not in stock false We get the sequence which is actived by clb5 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATCCTGAGTGCGAGCTTT downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTTCAGTAAAGGTGTTCAG false Kequan Lin BBa_K1051362 1 BBa_K1051362 K1051302(clb5 promoter) + K1051002 (non stop codon EYFP) + K1051008 (Stop codon + CAA terminator) 2013-07-18T11:00:00Z 2015-05-08T01:08:54Z 1335bp clb5 promoter+EYFP+stop codon false false _1358_ 0 15940 9 Not in stock false biobrick false Kequan Lin component2378381 1 BBa_K1051008 component2378379 1 BBa_K1051302 component2378380 1 BBa_K1051002 annotation2378381 1 BBa_K1051008 range2378381 1 1380 1397 annotation2378379 1 BBa_K1051302 range2378379 1 1 648 annotation2378380 1 BBa_K1051002 range2378380 1 655 1371 BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051002_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag BBa_K1051008_sequence 1 taatttttattcattttt BBa_K1051302_sequence 1 atggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttcttttttagactactaattttattttgctagtcattttttttttatactcaaaaagtaaaaagactacgagtatattcaaagtaaaaaacgaacgtcaaactatctcgattaaaacttgtcatactgtgggtatcatattctgtgccctcagtgaaaagaaccagcaaaagaacgcgcatctcgagtgaagacgcgcccttgatggtacaaaatttaacgggaaggcgcgtcgtgatgttcacgcgctttgcccacattgggatagcgcccacagcatatctgtgctaaactcacttttcctagtgactgccgatagctactgccatctaccgcgaagggaacttcatttgcgttcatcggtttattagaagctacttggaactaattcttaagcttctcaagaaaagttttttttctgtctatctattgaagtctttttgtctttgtacttcaagagactcaatcacctaaagcttttcacggccaattagttgtctcacacaaagcaaaataagcttaataattagcagtaacgcgcttttccctgtatttaaagccgctgaacacctttactgaaca BBa_K1051362_sequence 1 atggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttcttttttagactactaattttattttgctagtcattttttttttatactcaaaaagtaaaaagactacgagtatattcaaagtaaaaaacgaacgtcaaactatctcgattaaaacttgtcatactgtgggtatcatattctgtgccctcagtgaaaagaaccagcaaaagaacgcgcatctcgagtgaagacgcgcccttgatggtacaaaatttaacgggaaggcgcgtcgtgatgttcacgcgctttgcccacattgggatagcgcccacagcatatctgtgctaaactcacttttcctagtgactgccgatagctactgccatctaccgcgaagggaacttcatttgcgttcatcggtttattagaagctacttggaactaattcttaagcttctcaagaaaagttttttttctgtctatctattgaagtctttttgtctttgtacttcaagagactcaatcacctaaagcttttcacggccaattagttgtctcacacaaagcaaaataagcttaataattagcagtaacgcgcttttccctgtatttaaagccgctgaacacctttactgaacatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtactagagtaatttttattcattttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z