BBa_K1051300 1 BBa_K1051300 cln2 promoter (G1 phase to S phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z ATTTGTCTCAACGGAAGTACACGAAATTCGTCCCGCTGAACCTCAAAACTGCGTGTTCTA GTCACGAAACGCGCCAAAAACCGTTATGTTTCCGCATGCGGATACCTAGCGAAGAGCACA TTTGGCGTAGCCTGTTAAGTATAACTGCTAAAAATTGAAATGAACACCAACTGAAACAAC TGTTGGCCCTTTTTGAGGATCTAACCTGCGAAATGTTGATCTGACAGGCCTACTGAAAAA CGGAAAGGAAAAAAGGCCTGCGCAAACGCAGAACGCAGATCCGCCTTCTATTTGCGTATC GCACGCAGCCTCTGGCTACTTGTTTAACTTACTATCTCTTTCATATATAAAAAAATAGCA ATCGACTCTGGTAACTATTATGCTCCTCTTACTGGTTTTTTAGTGAATTGGCAATAGATA ATATCAAGGACAACTTACTTTTATTTTAACATCTACTTTACTTCATCGTCAAATTTTCTT CTTATATACAGCATTGCTAGACTCTATAGCTGCCAATTCATTCGCTTACCACATCATAAT TTGCATACAAAAGAAAATCTTTTCTTTTTTCCATTCATTCATTAA ATTTA ACGACAGACA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 It's complicated false We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATTTGTCTCAACGGAAGTAC downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTCTGTCGTTAAATTTAAT false Kequan Lin BBa_K1051365 1 BBa_K1051365 K1051300 (cln2 promoter) + K1051003 (non stop codon GFP) + K1051006 (Stop codon + TBY-1 terminator) 2013-07-18T11:00:00Z 2015-05-08T01:08:54Z 1332bp cln2 promoter+GFP+stop codon false false _1358_ 0 15940 9 Not in stock false biobrick false Kequan Lin component2331817 1 BBa_K1051006 component2331815 1 BBa_K1051300 component2331816 1 BBa_K1051003 annotation2331817 1 BBa_K1051006 range2331817 1 1729 1759 annotation2331816 1 BBa_K1051003 range2331816 1 1007 1720 annotation2331815 1 BBa_K1051300 range2331815 1 1 1000 BBa_K1051003 1 BBa_K1051003 Stop codon free GFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit GFP without stop coding false false _1358_ 0 15912 9 In stock false add prefix suffix del stop coding false Xiang LI BBa_K1051006 1 BBa_K1051006 Saccharomycescerevisiae TBY-1 terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z Saccharomycescerevisiae Saccharomycescerevisiae terminator with stopcodon false false _1358_ 0 15912 9 It's complicated false stopcodon + terminator false Xiang LI BBa_K1051006_sequence 1 taatagtgtatgtgtataatatcatagttta BBa_K1051300_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagaca BBa_K1051365_sequence 1 ttttcgtgattttcaccgagcgacacaaaaacgggtcgcgtcagaaataattttaaaagagcagcgtaatataccgctacagaaaaattggttcaagtatccatttttaagcatctttgcttctatttactcttgaaaatattcctccagtatcgcctgtgttggagtgcggtaacttttattgttttctctacgagtgaaaggcattttttgaaaccactattcgtttccttctgtaagttatctcatctttttcgtatcctccgcacttttaccctgaaatgcgaactaaagcaactatacattgttaataatgatactgaggttcaaaagtgccaagttatcaattcatgcgcgctttacccggctccatctttccgaaaacggaaatcatcgcgaaatttgtctcaacggaagtacacgaaattcgtcccgctgaacctcaaaactgcgtgttctagtcacgaaacgcgccaaaaaccgttatgtttccgcatgcggatacctagcgaagagcacatttggcgtagcctgttaagtataactgctaaaaattgaaatgaacaccaactgaaacaactgttggccctttttgaggatctaacctgcgaaatgttgatctgacaggcctactgaaaaacggaaaggaaaaaaggcctgcgcaaacgcagaacgcagatccgccttctatttgcgtatcgcacgcagcctctggctacttgtttaacttactatctctttcatatataaaaaaatagcaatcgactctggtaactattatgctcctcttactggttttttagtgaattggcaatagataatatcaaggacaacttacttttattttaacatctactttacttcatcgtcaaattttcttcttatatacagcattgctagactctatagctgccaattcattcgcttaccacatcataatttgcatacaaaagaaaatcttttcttttttccattcattcattaaatttaacgacagacatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagagtaatagtgtatgtgtataatatcatagttta BBa_K1051003_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z