BBa_K1051304 1 BBa_K1051304 cln3 promoter (M phase to G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z GAAAAATTATCAGGCAAGAAAAAGAAATTACCAAGCCTGCTCTCACTGTAATGATCAAGT TACATAAATTTACTATCGGATTAGTGTGTCCCTCTGCCACATTTCCATATTTGGCCTTGG GTTTTTGCCCTCATCTTTTTTTTTTCTTCCTCCTACCTATTTATAATTTGTATATCTGTA CTTTCCTCGAGCTTTTAATCTTCTTCTTAAACATTTTCTTGTGTAGTATACTTTCACACA ATTTCTTTCTTGATTTTTTCTTCTACTACTGAGTCTGCCAGTCAAATGGATTTCTGAGGA AAGAGGACTATACCCATTAGGAAACGAATTGCCCGAGTAGTCTCCTCTGCCGACTTAAAC CAACCTTTTTCTATTTCTCTTTTCTTTTCTCCCTCTTTTTTCTCTGTACTAGCATCCAAA AGCAAGCATCCATCCGAGTCCCAGTCGCAATCTCACATCTCCAATTTAACGTATCCATTG CATTTCCTCATTCGGTTTAACTCCTCTGCATTTCTTTTCTGACCCATAGCATTTCTTACA TTCCATTGCATCTCCCTTTTACTCTCGTTCAAGACACTGATTTGATACGCTTTCTGTACG It is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 In stock false We get the sequence which is actived by cln3 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream: downstream: false Kequan Lin BBa_K1051369 1 BBa_K1051369 K1051304 (cln3 promoter) + K1051003 (non stop codon GFP) + K1051006 (Stop codon + TBY-1 terminator) 2013-07-18T11:00:00Z 2015-05-08T01:08:55Z 1332bp cln3 promoter+GFP+stop codon false false _1358_ 0 15940 9 It's complicated false biobrick false Kequan Lin component2331830 1 BBa_K1051304 component2331832 1 BBa_K1051006 component2331831 1 BBa_K1051003 annotation2331831 1 BBa_K1051003 range2331831 1 1082 1795 annotation2331830 1 BBa_K1051304 range2331830 1 1 1075 annotation2331832 1 BBa_K1051006 range2331832 1 1804 1834 BBa_K1051003 1 BBa_K1051003 Stop codon free GFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit GFP without stop coding false false _1358_ 0 15912 9 In stock false add prefix suffix del stop coding false Xiang LI BBa_K1051006 1 BBa_K1051006 Saccharomycescerevisiae TBY-1 terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z Saccharomycescerevisiae Saccharomycescerevisiae terminator with stopcodon false false _1358_ 0 15912 9 It's complicated false stopcodon + terminator false Xiang LI BBa_K1051006_sequence 1 taatagtgtatgtgtataatatcatagttta BBa_K1051304_sequence 1 cacctttatcgtgtgccacggcggtaaaaaactaccttcaggggtacgatacattctgtgctggcgaccacggggctgacagagacacccgtagaggctacattactgatttgggaaatttcccaaattggaaatatcacctcgtcgacgtgctgcggtgcatggctgttttacccgtttaggaaaaaactcggcgggttttcttgacgggcaaatgtcgccattcgtctcgtttgaacgcttggcctgtaaaacagctaactcattcactatctctatcctgcgaccgtgatgataccgaaagtatgctgatacagatcggtccctcatatcgcgatagggctttctgagctctcctcccccttcttccctccccagccaaagaggccggttttccttctggggaagtgtcgcaaccaaacgctgtgctcaaaccctaaattgtgaactttcaagaaaaaaaaaaagaaaaagtgaaaaattatcaggcaagaaaaagaaattaccaagcctgctctcactgtaatgatcaagttacataaatttactatcggattagtgtgtccctctgccacatttccatatttggccttgggtttttgccctcatcttttttttttcttcctcctacctatttataatttgtatatctgtactttcctcgagcttttaatcttcttcttaaacattttcttgtgtagtatactttcacacaatttctttcttgattttttcttctactactgagtctgccagtcaaatggatttctgaggaaagaggactatacccattaggaaacgaattgcccgagtagtctcctctgccgacttaaaccaacctttttctatttctcttttcttttctccctcttttttctctgtactagcatccaaaagcaagcatccatccgagtcccagtcgcaatctcacatctccaatttaacgtatccattgcatttcctcattcggtttaactcctctgcatttcttttctgacccatagcatttcttacattccattgcatctcccttttactctcgttcaagacactgatttgatacgctttctgtacg BBa_K1051003_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa BBa_K1051369_sequence 1 cacctttatcgtgtgccacggcggtaaaaaactaccttcaggggtacgatacattctgtgctggcgaccacggggctgacagagacacccgtagaggctacattactgatttgggaaatttcccaaattggaaatatcacctcgtcgacgtgctgcggtgcatggctgttttacccgtttaggaaaaaactcggcgggttttcttgacgggcaaatgtcgccattcgtctcgtttgaacgcttggcctgtaaaacagctaactcattcactatctctatcctgcgaccgtgatgataccgaaagtatgctgatacagatcggtccctcatatcgcgatagggctttctgagctctcctcccccttcttccctccccagccaaagaggccggttttccttctggggaagtgtcgcaaccaaacgctgtgctcaaaccctaaattgtgaactttcaagaaaaaaaaaaagaaaaagtgaaaaattatcaggcaagaaaaagaaattaccaagcctgctctcactgtaatgatcaagttacataaatttactatcggattagtgtgtccctctgccacatttccatatttggccttgggtttttgccctcatcttttttttttcttcctcctacctatttataatttgtatatctgtactttcctcgagcttttaatcttcttcttaaacattttcttgtgtagtatactttcacacaatttctttcttgattttttcttctactactgagtctgccagtcaaatggatttctgaggaaagaggactatacccattaggaaacgaattgcccgagtagtctcctctgccgacttaaaccaacctttttctatttctcttttcttttctccctcttttttctctgtactagcatccaaaagcaagcatccatccgagtcccagtcgcaatctcacatctccaatttaacgtatccattgcatttcctcattcggtttaactcctctgcatttcttttctgacccatagcatttcttacattccattgcatctcccttttactctcgttcaagacactgatttgatacgctttctgtacgtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagagtaatagtgtatgtgtataatatcatagttta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z