BBa_K1051004 1 BBa_K1051004 Stop codon free mOrange in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit morange without stopcodon false false _1358_ 0 15912 9 In stock false add prefix suffix del stop codon false Xiang LI BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051371 1 BBa_K1051371 K1051301 (clb2 promoter) + K1051004 (non stop codon mOrange) + K1051008 (Stop codon + CAA terminator 2013-07-18T11:00:00Z 2015-05-08T01:08:55Z 1356bp clb2 promoter+mOrange+stop codon false false _1358_ 0 15940 9 Not in stock false biobrick false Kequan Lin component2378391 1 BBa_K1051301 component2378392 1 BBa_K1051004 component2378393 1 BBa_K1051008 annotation2378393 1 BBa_K1051008 range2378393 1 1816 1833 annotation2378391 1 BBa_K1051301 range2378391 1 1 1063 annotation2378392 1 BBa_K1051004 range2378392 1 1070 1807 BBa_K1051301 1 BBa_K1051301 clb2 promoter (during G2 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z CTAGC ATCTC ATAGC ATGGG CATAGAGTTTCAGAAATTTTGCTCTTAATGGAAAATATAA CCTCTTTGGGGAAAAGAGAAATAGATCCTTTCTCAAAAATTTAGAAAGGAAAAAAAATCA AAATATAATAAAATAGATTTTTTTTAGGTTAATTTTTTGCCAATTTAGCTAGACTTTTGA AAAGATCCAAAAATTTACTAAAATTGAATCTTTCTGGTATTAATTTTGTCCTATACCTCA AGAAGTAAAGTATTCTCGAATTGATACTTCTGAAAAGCGAAAGAAATATAGCTAAAGGAT ACTGGTTATTTATTCTTTCTTTTTTTCTTTCTTTTTGATTGAGCCAGTGTATAATAAAAA CCTACTGCGAGCTATTCAATAAATTGACATAGAATATCTGTTGAAGCGGTTCTTTGATTG AGCATCTGTTTTCCTTTTGTATTTTTGTCCTTTTAGATTCTTTTAACGACATTGGAGAAA TTTTAATCATTGTATAAAAACTTCATTCGCTCGTTTGTCAGAAGTTTTTTTATAACTTGC TATTTCCTTCCAAGAAGCCTTTTATTGATTACCCCCTCTCTCTCTTCATTGATCTTATAG It is a promoter which promotes transciption in G2 of the yeast cell cycle .If you want to express your proteins in G2 of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 Not in stock false We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTAGCATCTCATAGCATGGG downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTACTATAAGATCAATGAAGAGA false Kequan Lin BBa_K1051004_sequence 1 atggcaactagcggcatggttagtaaaggagaagaaaacaatatggcaatcataaaagaatttatgagattcaaagtcagaatggaaggttctgtaaatggtcacgagttcgaaatagaaggggaaggtgaaggtagaccctatgaaggctttcaaacggctaaattaaaagttaccaagggtggaccattgccctttgcttgggatatcctgtctcctcagttcacttatggtagtaaagcctatgttaagcatcctgctgatattcctgattacttcaagttgagttttccagaaggtttcaaatgggagagagttatgaattttgaagatggcggagttgtgacagtgacacaagactcctcacttcaagacggtgagtttatttacaaggtaaaactacgtggcactaactttccgtcggatggaccagtcatgcaaaaaaagacgatgggttgggaggcttcatctgagcgaatgtatccagaagatggggcactaaagggcgaaattaagatgaggctcaaattaaaggatggtggacattatacctcggaagtgaaaactacctataaagccaaaaagccagttcaattacctggtgcatacattgttggcattaagttggacatcacaagccacaatgaagattatacaatagtagagcagtacgaacgcgcggaaggtaggcattctactggaggcatggatgaactatacaaaggttctggtaccgca BBa_K1051301_sequence 1 ttttgttatccctgttgttgttgttttataagtcctaatctcgcttgaatatcgcccatacttaccctgttcttgaccgtctactaaggtgccttagggggactcaaacttaagatcgccttctacgcgttttatgctatgatcattagtgttgatgcatcggaagtttaaaaattaagtgcgagtgcattagcacagtggaattattagaatgaccactactccttctaatcaaacacgcggaaatagccgccaaaagacagattttattccaaatgcgggtaactatttgtataatatgtttacatattgagcccgtttaggaaagtgcaagttcaaggcactaatcaaaaaaggagatttgtaaatatagcgaccgaatcaggaaaaggtcaacaacgaagttcgcgatatggatgaacttcggtgcctgtccctcgaggaaatagcaaactcgttacagctagcatctcatagcatgggcatagagtttcagaaattttgctcttaatggaaaatataacctctttggggaaaagagaaatagatcctttctcaaaaatttagaaaggaaaaaaaatcaaaatataataaaatagattttttttaggttaattttttgccaatttagctagacttttgaaaagatccaaaaatttactaaaattgaatctttctggtattaattttgtcctatacctcaagaagtaaagtattctcgaattgatacttctgaaaagcgaaagaaatatagctaaaggatactggttatttattctttctttttttctttctttttgattgagccagtgtataataaaaacctactgcgagctattcaataaattgacatagaatatctgttgaagcggttctttgattgagcatctgttttccttttgtatttttgtccttttagattcttttaacgacattggagaaattttaatcattgtataaaaacttcattcgctcgtttgtcagaagtttttttataacttgctatttccttccaagaagccttttattgattaccccctctctctcttcattgatcttatag BBa_K1051008_sequence 1 taatttttattcattttt BBa_K1051371_sequence 1 ttttgttatccctgttgttgttgttttataagtcctaatctcgcttgaatatcgcccatacttaccctgttcttgaccgtctactaaggtgccttagggggactcaaacttaagatcgccttctacgcgttttatgctatgatcattagtgttgatgcatcggaagtttaaaaattaagtgcgagtgcattagcacagtggaattattagaatgaccactactccttctaatcaaacacgcggaaatagccgccaaaagacagattttattccaaatgcgggtaactatttgtataatatgtttacatattgagcccgtttaggaaagtgcaagttcaaggcactaatcaaaaaaggagatttgtaaatatagcgaccgaatcaggaaaaggtcaacaacgaagttcgcgatatggatgaacttcggtgcctgtccctcgaggaaatagcaaactcgttacagctagcatctcatagcatgggcatagagtttcagaaattttgctcttaatggaaaatataacctctttggggaaaagagaaatagatcctttctcaaaaatttagaaaggaaaaaaaatcaaaatataataaaatagattttttttaggttaattttttgccaatttagctagacttttgaaaagatccaaaaatttactaaaattgaatctttctggtattaattttgtcctatacctcaagaagtaaagtattctcgaattgatacttctgaaaagcgaaagaaatatagctaaaggatactggttatttattctttctttttttctttctttttgattgagccagtgtataataaaaacctactgcgagctattcaataaattgacatagaatatctgttgaagcggttctttgattgagcatctgttttccttttgtatttttgtccttttagattcttttaacgacattggagaaattttaatcattgtataaaaacttcattcgctcgtttgtcagaagtttttttataacttgctatttccttccaagaagccttttattgattaccccctctctctcttcattgatcttatagtactagatggcaactagcggcatggttagtaaaggagaagaaaacaatatggcaatcataaaagaatttatgagattcaaagtcagaatggaaggttctgtaaatggtcacgagttcgaaatagaaggggaaggtgaaggtagaccctatgaaggctttcaaacggctaaattaaaagttaccaagggtggaccattgccctttgcttgggatatcctgtctcctcagttcacttatggtagtaaagcctatgttaagcatcctgctgatattcctgattacttcaagttgagttttccagaaggtttcaaatgggagagagttatgaattttgaagatggcggagttgtgacagtgacacaagactcctcacttcaagacggtgagtttatttacaaggtaaaactacgtggcactaactttccgtcggatggaccagtcatgcaaaaaaagacgatgggttgggaggcttcatctgagcgaatgtatccagaagatggggcactaaagggcgaaattaagatgaggctcaaattaaaggatggtggacattatacctcggaagtgaaaactacctataaagccaaaaagccagttcaattacctggtgcatacattgttggcattaagttggacatcacaagccacaatgaagattatacaatagtagagcagtacgaacgcgcggaaggtaggcattctactggaggcatggatgaactatacaaaggttctggtaccgcatactagagtaatttttattcattttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z