LOCUS BBa_K1051800 4164 bp DNA linear 22-Dec-2024 DEFINITION protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon. ACCESSION BBa_K1051800 VERSION BBa_K1051800.1 FEATURES Location/Qualifiers ORIGIN 1 atggacaaga agtacagcat tgggctcgcc atcggcacaa acagcgtcgg ctgggccgtc 61 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgaccgc 121 cacagcataa agaagaacct cattggtgcc ctcctgttcg actccgggga gacggccgaa 181 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc 241 tacctccagg agatttttag taatgagatg gctaaggtgg atgactcttt cttccatagg 301 ctggaggagt cctttttggt ggaggaggat aaaaagcatg agcgccaccc aatctttggc 361 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag 421 aagctggtag acagtaccga taaggctgac ttgcggttga tctatctcgc gctggcgcat 481 atgatcaagt ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat 541 gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga ggagaacccg 601 atcaacgcat ctggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg 661 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat 721 cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa 781 gatgccaagc tgcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc 841 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt 901 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt 961 atgatcaagc gctatgatga gcaccaccag gacttgactt tgctgaaggc ccttgtcaga 1021 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc 1081 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg 1141 gaaaaaatgg acggcaccga ggagctgctg gtaaagctga acagagaaga cctgctgcgc 1201 aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac 1261 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt 1321 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg cggaaattcc 1381 agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa 1441 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa 1501 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgaata cttcacagtt 1561 tacaacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg 1621 tctggagagc agaagaaagc tatcgtggac ctcctcttca agaccaaccg gaaagttacc 1681 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc 1741 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc 1801 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc 1861 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct 1921 catctcttcg acgacaaagt gatgaaacag ctcaagagac gccgatatac aggatggggg 1981 cggctgtcaa gaaaactgat caatggcatc cgagacaagc agagtggaaa gacaatcctg 2041 gattttctga agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac 2101 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggtcaggg ggacagtctt 2161 catgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actacaaacc 2221 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt 2281 atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg 2341 atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatcctgaa ggaacaccca 2401 gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctaca aaacggcagg 2461 gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatgcc 2521 atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc 2581 gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa 2641 aattattggc ggcagctgtt gaacgccaaa ctgatcacac aacggaagtt cgataatctg 2701 actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag 2761 cttgttgaga cacgccagat caccaagcat gtggcccaaa ttctcgattc acgcatgaac 2821 accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct 2881 aagctggtct cagatttcag aaaggacttt cagttttaca aggtgagaga gatcaacaat 2941 taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa 3001 tatcccaagc tggaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa 3061 atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc 3121 aacatcatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga 3181 ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc 3241 gccacagtgc gtaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta 3301 cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc 3361 gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct 3421 tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc 3481 aaggaactgc tgggcatcac aatcatggag cgatcatcct tcgagaaaaa ccccatcgac 3541 tttctggagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gctgcccaag 3601 tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagcta 3661 caaaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc 3721 cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa 3781 caacacaaac actaccttga tgagatcatc gagcaaataa gcgagttctc caaaagagtg 3841 atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag 3901 cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcc 3961 cctgccgcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag 4021 gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc 4081 gacctctctc agctcggtgg agacggcagc ggttccccca agaaaaaacg caaggtggaa 4141 gatcctaaga aaaagcggaa agtg //