BBa_K1051900
type: ComponentDefinition
persistent identity: BBa K1051900
display id: BBa_K1051900
version: 1
was generated by: iGEM to SBOL conversion
description: G1 phase magic with cell synchronization device and CRISPRi induced alternative splicing device.
created: 2013-09-24T11:00:00Z
modified: 2015-05-08T01:08:55Z
owned by: james
owned by: myers
mutable description: clb5 +d-box +GFP + src1 intron + tom40 + cca + gal + dCas9 + cca + snr52 + sgRNA(Hub1) + sup4
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
star: false
creator: Yang Zhou
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K1051971
component: BBa_K1051505
component: BBa_K1051851
sequence annotation: BBa_K1051971
sequence annotation: BBa_K1051851
sequence annotation: BBa_K1051505
sequence: https://synbiohub.org/public/igem/BBa_K1051900_sequence/1

BBa_K1051971
type: Component
persistent identity: component2367209
display id: component2367209
version: 1
top level: BBa_K1051900
definition: BBa_K1051971
access: public

BBa_K1051505
type: Component
persistent identity: component2367220
display id: component2367220
version: 1
top level: BBa_K1051900
definition: BBa_K1051505
access: public

BBa_K1051851
type: Component
persistent identity: component2367215
display id: component2367215
version: 1
top level: BBa_K1051900
definition: BBa_K1051851
access: public

BBa_K1051971
type: SequenceAnnotation
persistent identity: annotation2367209
display id: annotation2367209
version: 1
top level: BBa_K1051900
location: https://synbiohub.org/public/igem/BBa_K1051900/annotation2367209/range2367209/1
component: BBa_K1051971

https://synbiohub.org/public/igem/BBa_K1051900/annotation2367209/range2367209/1
type: Range
persistent identity: range2367209
display id: range2367209
version: 1
top level: BBa_K1051900
direction: direction/reverse
start: 1
end: 1785
orientation: inline

BBa_K1051851
type: SequenceAnnotation
persistent identity: annotation2367215
display id: annotation2367215
version: 1
top level: BBa_K1051900
location: https://synbiohub.org/public/igem/BBa_K1051900/annotation2367215/range2367215/1
component: BBa_K1051851

https://synbiohub.org/public/igem/BBa_K1051900/annotation2367215/range2367215/1
type: Range
persistent identity: range2367215
display id: range2367215
version: 1
top level: BBa_K1051900
direction: direction/reverse
start: 1794
end: 6934
orientation: inline

BBa_K1051505
type: SequenceAnnotation
persistent identity: annotation2367220
display id: annotation2367220
version: 1
top level: BBa_K1051900
location: https://synbiohub.org/public/igem/BBa_K1051900/annotation2367220/range2367220/1
component: BBa_K1051505

https://synbiohub.org/public/igem/BBa_K1051900/annotation2367220/range2367220/1
type: Range
persistent identity: range2367220
display id: range2367220
version: 1
top level: BBa_K1051900
direction: direction/reverse
start: 6943
end: 8391
orientation: inline

BBa_K1051505
type: ComponentDefinition
persistent identity: BBa K1051505
display id: BBa_K1051505
version: 1
was generated by: iGEM to SBOL conversion
description: GAL1- > Sic1- > TBY-1
created: 2013-07-04T11:00:00Z
modified: 2015-05-08T01:08:55Z
mutable provenance: It come from saccharomyces cerevisiae.
owned by: james
owned by: myers
mutable description: This part mainly contain a promoter of GAL, a gene of Sic1 and a terminator of ADH1. It can use to regulate cell cycle, prolong the cell cycle.
discontinued: false
dominant: false
experience: experience/None
group u list: _1358_
m user id: 0
owner id: 15918
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: mutate the multi-phosphorylation site of Sic1.
star: false
creator: Lin Qiongfen
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1051006
component: BBa_S05180
sequence annotation: BBa_S05180
sequence annotation: BBa_K1051006
sequence: https://synbiohub.org/public/igem/BBa_K1051505_sequence/1

BBa_K1051006
type: Component
persistent identity: component2332619
display id: component2332619
version: 1
top level: BBa_K1051505
definition: BBa_K1051006
access: public

BBa_S05180
type: Component
persistent identity: component2332618
display id: component2332618
version: 1
top level: BBa_K1051505
definition: BBa_S05180
access: public

BBa_S05180
type: SequenceAnnotation
persistent identity: annotation2332618
display id: annotation2332618
version: 1
top level: BBa_K1051505
location: https://synbiohub.org/public/igem/BBa_K1051505/annotation2332618/range2332618/1
component: BBa_S05180

https://synbiohub.org/public/igem/BBa_K1051505/annotation2332618/range2332618/1
type: Range
persistent identity: range2332618
display id: range2332618
version: 1
top level: BBa_K1051505
direction: direction/reverse
start: 1
end: 1410
orientation: inline

BBa_K1051006
type: SequenceAnnotation
persistent identity: annotation2332619
display id: annotation2332619
version: 1
top level: BBa_K1051505
location: https://synbiohub.org/public/igem/BBa_K1051505/annotation2332619/range2332619/1
component: BBa_K1051006

https://synbiohub.org/public/igem/BBa_K1051505/annotation2332619/range2332619/1
type: Range
persistent identity: range2332619
display id: range2332619
version: 1
top level: BBa_K1051505
direction: direction/reverse
start: 1419
end: 1449
orientation: inline

BBa_K1051971
type: ComponentDefinition
persistent identity: BBa K1051971
display id: BBa_K1051971
version: 1
was generated by: iGEM to SBOL conversion
description: clb6 P + D-box + GFP + I701 + TOM40 + T
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:55Z
mutable provenance: No
owned by: james
owned by: myers
mutable description: clb5 Promoter + D-box + GFP + SRC1 Intron+GG + Targeting Peptide to Mitochondria outer membrane + T
discontinued: false
dominant: false
experience: experience/None
group u list: _1358_
m user id: 0
owner id: 15920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No
star: false
creator: Yang Zhou
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_S05198
component: BBa_S05201
sequence annotation: BBa_S05201
sequence annotation: BBa_S05198
sequence: https://synbiohub.org/public/igem/BBa_K1051971_sequence/1

BBa_S05198
type: Component
persistent identity: component2367162
display id: component2367162
version: 1
top level: BBa_K1051971
definition: BBa_S05198
access: public

BBa_S05201
type: Component
persistent identity: component2367155
display id: component2367155
version: 1
top level: BBa_K1051971
definition: BBa_S05201
access: public

BBa_S05201
type: SequenceAnnotation
persistent identity: annotation2367155
display id: annotation2367155
version: 1
top level: BBa_K1051971
location: https://synbiohub.org/public/igem/BBa_K1051971/annotation2367155/range2367155/1
component: BBa_S05201

https://synbiohub.org/public/igem/BBa_K1051971/annotation2367155/range2367155/1
type: Range
persistent identity: range2367155
display id: range2367155
version: 1
top level: BBa_K1051971
direction: direction/reverse
start: 1
end: 746
orientation: inline

BBa_S05198
type: SequenceAnnotation
persistent identity: annotation2367162
display id: annotation2367162
version: 1
top level: BBa_K1051971
location: https://synbiohub.org/public/igem/BBa_K1051971/annotation2367162/range2367162/1
component: BBa_S05198

https://synbiohub.org/public/igem/BBa_K1051971/annotation2367162/range2367162/1
type: Range
persistent identity: range2367162
display id: range2367162
version: 1
top level: BBa_K1051971
direction: direction/reverse
start: 753
end: 1785
orientation: inline

BBa_K1051851
type: ComponentDefinition
persistent identity: BBa K1051851
display id: BBa_K1051851
version: 1
was generated by: iGEM to SBOL conversion
description: k1051850+k1051801
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:08:55Z
mutable provenance: k1051850,k1051801
owned by: james
owned by: myers
mutable description: Devices express dCas 9 protein and sgRNA targeting to gene HUBI.
discontinued: false
dominant: false
group u list: _1358_
m user id: 0
owner id: 15924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: no
star: false
creator: Shihong Chen
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1051801
component: BBa_K1051850
sequence annotation: BBa_K1051850
sequence annotation: BBa_K1051801
sequence: https://synbiohub.org/public/igem/BBa_K1051851_sequence/1

BBa_K1051801
type: Component
persistent identity: component2360231
display id: component2360231
version: 1
top level: BBa_K1051851
definition: BBa_K1051801
access: public

BBa_K1051850
type: Component
persistent identity: component2360230
display id: component2360230
version: 1
top level: BBa_K1051851
definition: BBa_K1051850
access: public

BBa_K1051850
type: SequenceAnnotation
persistent identity: annotation2360230
display id: annotation2360230
version: 1
top level: BBa_K1051851
location: https://synbiohub.org/public/igem/BBa_K1051851/annotation2360230/range2360230/1
component: BBa_K1051850

https://synbiohub.org/public/igem/BBa_K1051851/annotation2360230/range2360230/1
type: Range
persistent identity: range2360230
display id: range2360230
version: 1
top level: BBa_K1051851
direction: direction/reverse
start: 1
end: 4745
orientation: inline

BBa_K1051801
type: SequenceAnnotation
persistent identity: annotation2360231
display id: annotation2360231
version: 1
top level: BBa_K1051851
location: https://synbiohub.org/public/igem/BBa_K1051851/annotation2360231/range2360231/1
component: BBa_K1051801

https://synbiohub.org/public/igem/BBa_K1051851/annotation2360231/range2360231/1
type: Range
persistent identity: range2360231
display id: range2360231
version: 1
top level: BBa_K1051851
direction: direction/reverse
start: 4754
end: 5141
orientation: inline

BBa_K1051006
type: ComponentDefinition
persistent identity: BBa K1051006
display id: BBa_K1051006
version: 1
was generated by: iGEM to SBOL conversion
description: Saccharomycescerevisiae TBY-1 terminator with stopcodon
created: 2013-07-07T11:00:00Z
modified: 2015-05-08T01:08:53Z
mutable provenance: Saccharomycescerevisiae
owned by: james
owned by: myers
mutable description: Saccharomycescerevisiae terminator with stopcodon
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15912
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: stopcodon + terminator
star: false
creator: Xiang LI
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1051006_sequence/1

BBa_S05180
type: ComponentDefinition
persistent identity: BBa S05180
display id: BBa_S05180
version: 1
was generated by: iGEM to SBOL conversion
description: J63006:K1051500
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:14:50Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 11943
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Kang KANG
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K1051500
component: BBa_J63006
sequence annotation: BBa_J63006
sequence annotation: BBa_K1051500
sequence: https://synbiohub.org/public/igem/BBa_S05180_sequence/1

BBa_K1051500
type: Component
persistent identity: component2332615
display id: component2332615
version: 1
top level: BBa_S05180
definition: BBa_K1051500
access: public

BBa_J63006
type: Component
persistent identity: component2332614
display id: component2332614
version: 1
top level: BBa_S05180
definition: GAL1
access: public

BBa_J63006
type: SequenceAnnotation
persistent identity: annotation2332614
display id: annotation2332614
version: 1
top level: BBa_S05180
location: https://synbiohub.org/public/igem/BBa_S05180/annotation2332614/range2332614/1
component: BBa_J63006

https://synbiohub.org/public/igem/BBa_S05180/annotation2332614/range2332614/1
type: Range
persistent identity: range2332614
display id: range2332614
version: 1
top level: BBa_S05180
direction: direction/reverse
start: 1
end: 549
orientation: inline

BBa_K1051500
type: SequenceAnnotation
persistent identity: annotation2332615
display id: annotation2332615
version: 1
top level: BBa_S05180
location: https://synbiohub.org/public/igem/BBa_S05180/annotation2332615/range2332615/1
component: BBa_K1051500

https://synbiohub.org/public/igem/BBa_S05180/annotation2332615/range2332615/1
type: Range
persistent identity: range2332615
display id: range2332615
version: 1
top level: BBa_S05180
direction: direction/reverse
start: 556
end: 1410
orientation: inline

BBa_K1051801
type: ComponentDefinition
persistent identity: BBa K1051801
display id: BBa_K1051801
version: 1
was generated by: iGEM to SBOL conversion
description: Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib
created: 2013-09-07T11:00:00Z
modified: 2015-05-08T01:08:55Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This part consists of promoter SNR52, sgRNA coding sequence and SUP4 terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1358_
m user id: 0
owner id: 15924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: no
star: false
creator: Shihong Chen
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1051801_sequence/1

BBa_S05198
type: ComponentDefinition
persistent identity: BBa S05198
display id: BBa_S05198
version: 1
was generated by: iGEM to SBOL conversion
description: S05194:K1051008
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:14:51Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 15920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yang Zhou
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K1051008
component: BBa_S05194
sequence annotation: BBa_S05194
sequence annotation: BBa_K1051008
sequence: https://synbiohub.org/public/igem/BBa_S05198_sequence/1

BBa_K1051008
type: Component
persistent identity: component2337816
display id: component2337816
version: 1
top level: BBa_S05198
definition: BBa_K1051008
access: public

BBa_S05194
type: Component
persistent identity: component2337815
display id: component2337815
version: 1
top level: BBa_S05198
definition: BBa_S05194
access: public

BBa_S05194
type: SequenceAnnotation
persistent identity: annotation2337815
display id: annotation2337815
version: 1
top level: BBa_S05198
location: https://synbiohub.org/public/igem/BBa_S05198/annotation2337815/range2337815/1
component: BBa_S05194

https://synbiohub.org/public/igem/BBa_S05198/annotation2337815/range2337815/1
type: Range
persistent identity: range2337815
display id: range2337815
version: 1
top level: BBa_S05198
direction: direction/reverse
start: 1
end: 1007
orientation: inline

BBa_K1051008
type: SequenceAnnotation
persistent identity: annotation2337816
display id: annotation2337816
version: 1
top level: BBa_S05198
location: https://synbiohub.org/public/igem/BBa_S05198/annotation2337816/range2337816/1
component: BBa_K1051008

https://synbiohub.org/public/igem/BBa_S05198/annotation2337816/range2337816/1
type: Range
persistent identity: range2337816
display id: range2337816
version: 1
top level: BBa_S05198
direction: direction/reverse
start: 1016
end: 1033
orientation: inline

BBa_S05201
type: ComponentDefinition
persistent identity: BBa S05201
display id: BBa_S05201
version: 1
was generated by: iGEM to SBOL conversion
description: K1051302:K1051201
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:14:51Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 15920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yang Zhou
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K1051201
component: BBa_K1051303
sequence annotation: BBa_K1051303
sequence annotation: BBa_K1051201
sequence: https://synbiohub.org/public/igem/BBa_S05201_sequence/1

BBa_K1051201
type: Component
persistent identity: component2367142
display id: component2367142
version: 1
top level: BBa_S05201
definition: BBa_K1051201
access: public

BBa_K1051303
type: Component
persistent identity: component2367141
display id: component2367141
version: 1
top level: BBa_S05201
definition: BBa_K1051303
access: public

BBa_K1051303
type: SequenceAnnotation
persistent identity: annotation2367141
display id: annotation2367141
version: 1
top level: BBa_S05201
location: https://synbiohub.org/public/igem/BBa_S05201/annotation2367141/range2367141/1
component: BBa_K1051303

https://synbiohub.org/public/igem/BBa_S05201/annotation2367141/range2367141/1
type: Range
persistent identity: range2367141
display id: range2367141
version: 1
top level: BBa_S05201
direction: direction/reverse
start: 1
end: 699
orientation: inline

BBa_K1051201
type: SequenceAnnotation
persistent identity: annotation2367142
display id: annotation2367142
version: 1
top level: BBa_S05201
location: https://synbiohub.org/public/igem/BBa_S05201/annotation2367142/range2367142/1
component: BBa_K1051201

https://synbiohub.org/public/igem/BBa_S05201/annotation2367142/range2367142/1
type: Range
persistent identity: range2367142
display id: range2367142
version: 1
top level: BBa_S05201
direction: direction/reverse
start: 708
end: 746
orientation: inline

BBa_K1051850
type: ComponentDefinition
persistent identity: BBa K1051850
display id: BBa_K1051850
version: 1
was generated by: iGEM to SBOL conversion
description: J63006(Gal I promoter)+k1051800(dCas 9)+k1051008(terminator)
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:08:55Z
mutable provenance: J63006 k1051800 k1051008
owned by: james
owned by: myers
mutable description: A device of dCas 9 protein expression.
discontinued: false
dominant: false
group u list: _1358_
m user id: 0
owner id: 15924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: no
star: false
creator: Shihong Chen
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J63006
component: BBa_K1051008
component: BBa_K1051800
sequence annotation: BBa_J63006
sequence annotation: BBa_K1051800
sequence annotation: BBa_K1051008
sequence: https://synbiohub.org/public/igem/BBa_K1051850_sequence/1

BBa_J63006
type: Component
persistent identity: component2360224
display id: component2360224
version: 1
top level: BBa_K1051850
definition: GAL1
access: public

BBa_K1051008
type: Component
persistent identity: component2360226
display id: component2360226
version: 1
top level: BBa_K1051850
definition: BBa_K1051008
access: public

BBa_K1051800
type: Component
persistent identity: component2360225
display id: component2360225
version: 1
top level: BBa_K1051850
definition: dCas 9
access: public

BBa_J63006
type: SequenceAnnotation
persistent identity: annotation2360224
display id: annotation2360224
version: 1
top level: BBa_K1051850
location: https://synbiohub.org/public/igem/BBa_K1051850/annotation2360224/range2360224/1
component: BBa_J63006

https://synbiohub.org/public/igem/BBa_K1051850/annotation2360224/range2360224/1
type: Range
persistent identity: range2360224
display id: range2360224
version: 1
top level: BBa_K1051850
direction: direction/reverse
start: 1
end: 549
orientation: inline

BBa_K1051800
type: SequenceAnnotation
persistent identity: annotation2360225
display id: annotation2360225
version: 1
top level: BBa_K1051850
location: https://synbiohub.org/public/igem/BBa_K1051850/annotation2360225/range2360225/1
component: BBa_K1051800

https://synbiohub.org/public/igem/BBa_K1051850/annotation2360225/range2360225/1
type: Range
persistent identity: range2360225
display id: range2360225
version: 1
top level: BBa_K1051850
direction: direction/reverse
start: 556
end: 4719
orientation: inline

BBa_K1051008
type: SequenceAnnotation
persistent identity: annotation2360226
display id: annotation2360226
version: 1
top level: BBa_K1051850
location: https://synbiohub.org/public/igem/BBa_K1051850/annotation2360226/range2360226/1
component: BBa_K1051008

https://synbiohub.org/public/igem/BBa_K1051850/annotation2360226/range2360226/1
type: Range
persistent identity: range2360226
display id: range2360226
version: 1
top level: BBa_K1051850
direction: direction/reverse
start: 4728
end: 4745
orientation: inline

BBa_K1051303
type: ComponentDefinition
persistent identity: BBa K1051303
display id: BBa_K1051303
version: 1
was generated by: iGEM to SBOL conversion
description: clb6 promoter (during G1 phase)
created: 2013-06-29T11:00:00Z
modified: 2015-05-08T01:08:54Z
mutable provenance: TAACT AGGTG GTCCT TCCAT GAAAGTTTTTTTTTTTTGAAGATGTTCTTTTTTTTTTTTT GGGCCTCATTACAATGTAAGAGGTTATTATGTGTTACATGAGTAAAGCTAATTGACGCAA GCAGGTAATGAAGAAAGCCCTATATTACCGTATAGACAATGGGCGCGTCCCTAAAGAGGA TACCAAATAGCAGTTTTACGCGTACCACAAAATTTAACGACCAACGCGTATTCTTCGCGT TTTTTTTTTGCGACGGTAAACAAAAAAAGGGCCCTCTGCGGTAAAACAAGTGGGTCTACC ACAGGACACAGGACTTGGTTGTTTCTACTCTCCCTGTGTATCCTACAATTGGCAATTGGC AATTCGCAACCTGCGTTTAAATATTTACCGTTTTAGCACATTGATTACCTTTTTTGCTAG GGTTAAGTTTTTGCCTTTTTCTTATTAACATATATATAACTGCTTCATTTCATACAAGTG ACTTTTATCTTTTATTAAATAATAACAAGCTTCACTAATCTAGCGTTCATACAACATACA AATCAGTGTGTTGAAAATTATTATTCTCTGATATTCTCTCCCTCCTTTTAAATTTTTAAA
owned by: james
owned by: myers
mutable description: It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15940
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We get the sequence which is actived by clb6 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTAACT AGGTG GTCCT TCCAT downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTAAAAATTTAAAAGGAGG
star: false
creator: Kequan Lin
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K1051303_sequence/1

BBa_K1051201
type: ComponentDefinition
persistent identity: BBa K1051201
display id: BBa_K1051201
version: 1
was generated by: iGEM to SBOL conversion
description: D-BOX
created: 2013-06-29T11:00:00Z
modified: 2015-05-08T01:08:54Z
mutable provenance: 5' - AATTC GCGGCCGC T TCTAGA GTACAAAGGTTGGCCTTAAACAATGTAACAAATACGACA ACTAGT A GCGGCCG CTGCA- 3' 5???- G CGGCCGC T ACTAGT TGTCGTATTTGTTACATTGTTTAAGGCCAACCTTTGTAC TCTAGAA GCGGCCGC G-3???
owned by: james
owned by: myers
mutable description: D-BOX
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 18073
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: D-BOX
star: false
creator: wanling ZHOU
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1051201_sequence/1

BBa_K1051500
type: ComponentDefinition
persistent identity: BBa K1051500
display id: BBa_K1051500
version: 1
was generated by: iGEM to SBOL conversion
description: Sic1 mutation
created: 2013-07-01T11:00:00Z
modified: 2015-05-08T01:08:55Z
mutable provenance: Come from saccharomyces cerevisiae.
owned by: james
owned by: myers
mutable description: Mutate seven phosphorylation site of Sic1, stopping the cell cycle in the G1 phase.
discontinued: false
dominant: false
group u list: _1358_
m user id: 0
owner id: 15918
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Mutate seven phosphorylation site of Sic1 and one site of Spe1's restriction site.
star: false
creator: Lin Qiongfen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1051500_sequence/1

BBa_K1051008
type: ComponentDefinition
persistent identity: BBa K1051008
display id: BBa_K1051008
version: 1
was generated by: iGEM to SBOL conversion
description: S. cerevisiae CAA terminator with stopcodon
created: 2013-07-07T11:00:00Z
modified: 2015-05-08T01:08:53Z
mutable provenance: S. cerevisiae
owned by: james
owned by: myers
mutable description: S. cerevisiae terminator with stopcodon
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15912
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: stopcodon + terminator
star: false
creator: Xiang LI
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1051008_sequence/1

dCas 9
type: ComponentDefinition
persistent identity: BBa K1051800
display id: BBa_K1051800
version: 1
was generated by: iGEM to SBOL conversion
description: protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.
created: 2013-07-10T11:00:00Z
modified: 2015-05-08T01:08:55Z
mutable provenance: The Cas9 gene was got from Mr. Kai Tian,BGI Shenzhen China. The gene was splited into three parts to synthetize optimizing the codons in eucaryon.
owned by: james
owned by: myers
mutable description: Protein dCas9 is the key of the technology CRISPRi,and this part is the gene of the dcas9.Delete its ending codon is to provide an convenient way to add other protein domain to improve the function of dCas9.
discontinued: false
dominant: false
experience: experience/None
group u list: _1358_
m user id: 0
owner id: 15924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To mutation four Restriction Enzyme cutting sites(SpeI) and two codons(D10A & H841A),we design PCR primers to mutate them by PCR using Phution DNA polymerase. Refrence http://www.ncbi.nlm.nih.gov/pubmed/?term=repurposing+CRISPR+as+an+RNA-Guided+Platfrom
star: false
creator: Shihong Chen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1051800_sequence/1

BBa_S05194
type: ComponentDefinition
persistent identity: BBa S05194
display id: BBa_S05194
version: 1
was generated by: iGEM to SBOL conversion
description: S05154:K1051105
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:14:51Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 15920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yang Zhou
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S05154
component: BBa_K1051105
sequence annotation: BBa_S05154
sequence annotation: BBa_K1051105
sequence: https://synbiohub.org/public/igem/BBa_S05194_sequence/1

BBa_S05154
type: Component
persistent identity: component2337791
display id: component2337791
version: 1
top level: BBa_S05194
definition: BBa_S05154
access: public

BBa_K1051105
type: Component
persistent identity: component2337792
display id: component2337792
version: 1
top level: BBa_S05194
definition: BBa_K1051105
access: public

BBa_S05154
type: SequenceAnnotation
persistent identity: annotation2337791
display id: annotation2337791
version: 1
top level: BBa_S05194
location: https://synbiohub.org/public/igem/BBa_S05194/annotation2337791/range2337791/1
component: BBa_S05154

https://synbiohub.org/public/igem/BBa_S05194/annotation2337791/range2337791/1
type: Range
persistent identity: range2337791
display id: range2337791
version: 1
top level: BBa_S05194
direction: direction/reverse
start: 1
end: 854
orientation: inline

BBa_K1051105
type: SequenceAnnotation
persistent identity: annotation2337792
display id: annotation2337792
version: 1
top level: BBa_S05194
location: https://synbiohub.org/public/igem/BBa_S05194/annotation2337792/range2337792/1
component: BBa_K1051105

https://synbiohub.org/public/igem/BBa_S05194/annotation2337792/range2337792/1
type: Range
persistent identity: range2337792
display id: range2337792
version: 1
top level: BBa_S05194
direction: direction/reverse
start: 861
end: 1007
orientation: inline

GAL1
type: ComponentDefinition
persistent identity: BBa J63006
display id: BBa_J63006
version: 1
was generated by: iGEM to SBOL conversion
description: yeast GAL1 promoter
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: genomic GAL1 locus
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GAL1 promoter from S. cerevisiae
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: In stock
status: status/Available
bookmark: false
mutable notes: unchanged from genomic sequence
star: true
creator: Caroline Ajo-Franklin
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J63006_sequence/1

BBa_S05154
type: ComponentDefinition
persistent identity: BBa S05154
display id: BBa_S05154
version: 1
was generated by: iGEM to SBOL conversion
description: GFP+I701
created: 2013-07-08T11:00:00Z
modified: 2015-05-08T01:14:50Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 15920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yang Zhou
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K1051701
component: BBa_K1051003
sequence annotation: BBa_K1051003
sequence annotation: BBa_K1051701
sequence: https://synbiohub.org/public/igem/BBa_S05154_sequence/1

BBa_K1051701
type: Component
persistent identity: component2332116
display id: component2332116
version: 1
top level: BBa_S05154
definition: BBa_K1051701
access: public

BBa_K1051003
type: Component
persistent identity: component2332115
display id: component2332115
version: 1
top level: BBa_S05154
definition: BBa_K1051003
access: public

BBa_K1051003
type: SequenceAnnotation
persistent identity: annotation2332115
display id: annotation2332115
version: 1
top level: BBa_S05154
location: https://synbiohub.org/public/igem/BBa_S05154/annotation2332115/range2332115/1
component: BBa_K1051003

https://synbiohub.org/public/igem/BBa_S05154/annotation2332115/range2332115/1
type: Range
persistent identity: range2332115
display id: range2332115
version: 1
top level: BBa_S05154
direction: direction/reverse
start: 1
end: 714
orientation: inline

BBa_K1051701
type: SequenceAnnotation
persistent identity: annotation2332116
display id: annotation2332116
version: 1
top level: BBa_S05154
location: https://synbiohub.org/public/igem/BBa_S05154/annotation2332116/range2332116/1
component: BBa_K1051701

https://synbiohub.org/public/igem/BBa_S05154/annotation2332116/range2332116/1
type: Range
persistent identity: range2332116
display id: range2332116
version: 1
top level: BBa_S05154
direction: direction/reverse
start: 723
end: 854
orientation: inline

BBa_K1051105
type: ComponentDefinition
persistent identity: BBa K1051105
display id: BBa_K1051105
version: 1
was generated by: iGEM to SBOL conversion
description: TOM40;targeting peptide to mitochondria outer membrane
created: 2013-07-11T11:00:00Z
modified: 2015-05-08T01:08:53Z
mutable provenance: Saccharomyces cerevisiae genomics sequence
owned by: james
owned by: myers
mutable description: After added with 23 prefix and suffix, it can be used as a transporter to the mitochondria inner membrane. Also, when joining with different flourescent proteins, they can light the inner membrane using such proteins.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15923
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: ATG was added at start
star: false
creator: Jinchun Zhang
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1051105_sequence/1

BBa_K1051701
type: ComponentDefinition
persistent identity: BBa K1051701
display id: BBa_K1051701
version: 1
was generated by: iGEM to SBOL conversion
description: SRC1 Intron+GG
created: 2013-06-29T11:00:00Z
modified: 2015-05-08T01:08:55Z
mutable provenance: From SRC1
owned by: james
owned by: myers
mutable description: Intron from SRC1 and is added GG to form a Ser codon
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1358_
m user id: 0
owner id: 15920
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no
star: false
creator: Yang Zhou
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1051701_sequence/1

BBa_K1051003
type: ComponentDefinition
persistent identity: BBa K1051003
display id: BBa_K1051003
version: 1
was generated by: iGEM to SBOL conversion
description: Stop codon free GFP in RFC[23] standard
created: 2013-07-03T11:00:00Z
modified: 2015-05-08T01:08:53Z
mutable provenance: kit
owned by: james
owned by: myers
mutable description: GFP without stop coding
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15912
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: add prefix suffix del stop coding
star: false
creator: Xiang LI
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1051003_sequence/1

https://synbiohub.org/public/igem/BBa_K1051900_sequence/1
type: Sequence
persistent identity: BBa K1051900 sequence
display id: BBa_K1051900_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagaggtacaaaggttggccttaaacaatgtaacaaatacgacatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttttactagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051505_sequence/1
type: Sequence
persistent identity: BBa K1051505 sequence
display id: BBa_K1051505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatgattccttccatcccaccaaggtccagagggactaggtaccttgcgcagcctagtggcaataccagttctagtgccctaatgcaaggtcaaaagatcccccaaaagccttcacagaacctagtccctgtcattccctcaacaactaagtcttttaaaaatgcgccattattagcacctcccaattcgaacatgggtatgaccactccatttaatgggcttacgactcctcaacgcacgccgtttccaaaatcttcagtgaagaggacactattccaatttgaaagtcatgataatggaacagtaagggaagagcaggaaccattgggtcgtgtaaataggatattgtttcccacgcagcaaaatgtggatatagatgcagcagaagaagaagaagaaggagaagttcttcttccccccagcagacctacatctgccaggcagttacatttatcacttgaaagagatgagtttgatcagacacatagaaagaagattattaaagatgtacctggtacgcccagcgacaaggtgataacatttgaattggcaaaaaattggaacaacaactctccgaaaaatgacgccaggagtcaagaaagtgaagacgaggaagacatcatcatcaatccagtgcgggtgggtaaaaatccctttgcatcagatgaactggtcactcaggaaattagaaatgaacgtaaaagggcaatgttgagagaaaacccagatatagaagacgtaataacatatgtcaataagaagggagaggtggtagagaaacgaaggttaacggatgaagaaaagagaagattcaagccaaaggcattgtttcaatctagggatcaagagcattgatactagagtaatagtgtatgtgtataatatcatagttta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051971_sequence/1
type: Sequence
persistent identity: BBa K1051971 sequence
display id: BBa_K1051971_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagaggtacaaaggttggccttaaacaatgtaacaaatacgacatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051851_sequence/1
type: Sequence
persistent identity: BBa K1051851 sequence
display id: BBa_K1051851_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatggacaagaagtacagcattgggctcgccatcggcacaaacagcgtcggctgggccgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccgaccgccacagcataaagaagaacctcattggtgccctcctgttcgactccggggagacggccgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgctacctccaggagatttttagtaatgagatggctaaggtggatgactctttcttccataggctggaggagtcctttttggtggaggaggataaaaagcatgagcgccacccaatctttggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaagaagctggtagacagtaccgataaggctgacttgcggttgatctatctcgcgctggcgcatatgatcaagtttcggggacacttcctcatcgagggggacctgaacccagacaacagcgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaggagaacccgatcaacgcatctggagttgacgccaaagcaatcctgagcgctaggctgtccaaatcccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttggtaatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaagatgccaagctgcaactgagcaaagacacctacgatgatgatctcgacaatctgctggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagtatgatcaagcgctatgatgagcaccaccaggacttgactttgctgaaggcccttgtcagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttggaaaaaatggacggcaccgaggagctgctggtaaagctgaacagagaagacctgctgcgcaaacagcgcactttcgacaatggaagcatcccccaccagattcacctgggcgaactgcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagattgagaaaatcctcacatttcggataccctactatgtaggccccctcgcccgcggaaattccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgaggaagtcgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051006_sequence/1
type: Sequence
persistent identity: BBa K1051006 sequence
display id: BBa_K1051006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatagtgtatgtgtataatatcatagttta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05180_sequence/1
type: Sequence
persistent identity: BBa S05180 sequence
display id: BBa_S05180_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatgattccttccatcccaccaaggtccagagggactaggtaccttgcgcagcctagtggcaataccagttctagtgccctaatgcaaggtcaaaagatcccccaaaagccttcacagaacctagtccctgtcattccctcaacaactaagtcttttaaaaatgcgccattattagcacctcccaattcgaacatgggtatgaccactccatttaatgggcttacgactcctcaacgcacgccgtttccaaaatcttcagtgaagaggacactattccaatttgaaagtcatgataatggaacagtaagggaagagcaggaaccattgggtcgtgtaaataggatattgtttcccacgcagcaaaatgtggatatagatgcagcagaagaagaagaagaaggagaagttcttcttccccccagcagacctacatctgccaggcagttacatttatcacttgaaagagatgagtttgatcagacacatagaaagaagattattaaagatgtacctggtacgcccagcgacaaggtgataacatttgaattggcaaaaaattggaacaacaactctccgaaaaatgacgccaggagtcaagaaagtgaagacgaggaagacatcatcatcaatccagtgcgggtgggtaaaaatccctttgcatcagatgaactggtcactcaggaaattagaaatgaacgtaaaagggcaatgttgagagaaaacccagatatagaagacgtaataacatatgtcaataagaagggagaggtggtagagaaacgaaggttaacggatgaagaaaagagaagattcaagccaaaggcattgtttcaatctagggatcaagagcattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051801_sequence/1
type: Sequence
persistent identity: BBa K1051801 sequence
display id: BBa_K1051801_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctttgaaaagataatgtatgattatgctttcactcatatttatacagaaacttgatgttttctttcgagtatatacaaggtgattacatgtacgtttgaagtacaacactagattttgtagtgccctcttgggctagcggtaaaggtgcgcattttttcacaccctacaatgttctgttcaaaagattttggtcaaacgctgtagaagtgaaagttggtgcgcatgtttcggcgttcgaaacttctccgcagtgaaagataaatgatcagttgtgaatgaccgattaggttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtggtgctttttttgttttttatgtct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05198_sequence/1
type: Sequence
persistent identity: BBa S05198 sequence
display id: BBa_S05198_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05201_sequence/1
type: Sequence
persistent identity: BBa S05201 sequence
display id: BBa_S05201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagaggtacaaaggttggccttaaacaatgtaacaaatacgaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051850_sequence/1
type: Sequence
persistent identity: BBa K1051850 sequence
display id: BBa_K1051850_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatggacaagaagtacagcattgggctcgccatcggcacaaacagcgtcggctgggccgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccgaccgccacagcataaagaagaacctcattggtgccctcctgttcgactccggggagacggccgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgctacctccaggagatttttagtaatgagatggctaaggtggatgactctttcttccataggctggaggagtcctttttggtggaggaggataaaaagcatgagcgccacccaatctttggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaagaagctggtagacagtaccgataaggctgacttgcggttgatctatctcgcgctggcgcatatgatcaagtttcggggacacttcctcatcgagggggacctgaacccagacaacagcgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaggagaacccgatcaacgcatctggagttgacgccaaagcaatcctgagcgctaggctgtccaaatcccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttggtaatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaagatgccaagctgcaactgagcaaagacacctacgatgatgatctcgacaatctgctggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagtatgatcaagcgctatgatgagcaccaccaggacttgactttgctgaaggcccttgtcagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttggaaaaaatggacggcaccgaggagctgctggtaaagctgaacagagaagacctgctgcgcaaacagcgcactttcgacaatggaagcatcccccaccagattcacctgggcgaactgcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagattgagaaaatcctcacatttcggataccctactatgtaggccccctcgcccgcggaaattccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgaggaagtcgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051303_sequence/1
type: Sequence
persistent identity: BBa K1051303 sequence
display id: BBa_K1051303_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051201_sequence/1
type: Sequence
persistent identity: BBa K1051201 sequence
display id: BBa_K1051201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtacaaaggttggccttaaacaatgtaacaaatacgaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051500_sequence/1
type: Sequence
persistent identity: BBa K1051500 sequence
display id: BBa_K1051500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattccttccatcccaccaaggtccagagggactaggtaccttgcgcagcctagtggcaataccagttctagtgccctaatgcaaggtcaaaagatcccccaaaagccttcacagaacctagtccctgtcattccctcaacaactaagtcttttaaaaatgcgccattattagcacctcccaattcgaacatgggtatgaccactccatttaatgggcttacgactcctcaacgcacgccgtttccaaaatcttcagtgaagaggacactattccaatttgaaagtcatgataatggaacagtaagggaagagcaggaaccattgggtcgtgtaaataggatattgtttcccacgcagcaaaatgtggatatagatgcagcagaagaagaagaagaaggagaagttcttcttccccccagcagacctacatctgccaggcagttacatttatcacttgaaagagatgagtttgatcagacacatagaaagaagattattaaagatgtacctggtacgcccagcgacaaggtgataacatttgaattggcaaaaaattggaacaacaactctccgaaaaatgacgccaggagtcaagaaagtgaagacgaggaagacatcatcatcaatccagtgcgggtgggtaaaaatccctttgcatcagatgaactggtcactcaggaaattagaaatgaacgtaaaagggcaatgttgagagaaaacccagatatagaagacgtaataacatatgtcaataagaagggagaggtggtagagaaacgaaggttaacggatgaagaaaagagaagattcaagccaaaggcattgtttcaatctagggatcaagagcattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051008_sequence/1
type: Sequence
persistent identity: BBa K1051008 sequence
display id: BBa_K1051008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatttttattcattttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051800_sequence/1
type: Sequence
persistent identity: BBa K1051800 sequence
display id: BBa_K1051800_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggacaagaagtacagcattgggctcgccatcggcacaaacagcgtcggctgggccgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccgaccgccacagcataaagaagaacctcattggtgccctcctgttcgactccggggagacggccgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgctacctccaggagatttttagtaatgagatggctaaggtggatgactctttcttccataggctggaggagtcctttttggtggaggaggataaaaagcatgagcgccacccaatctttggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaagaagctggtagacagtaccgataaggctgacttgcggttgatctatctcgcgctggcgcatatgatcaagtttcggggacacttcctcatcgagggggacctgaacccagacaacagcgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaggagaacccgatcaacgcatctggagttgacgccaaagcaatcctgagcgctaggctgtccaaatcccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttggtaatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaagatgccaagctgcaactgagcaaagacacctacgatgatgatctcgacaatctgctggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagtatgatcaagcgctatgatgagcaccaccaggacttgactttgctgaaggcccttgtcagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttggaaaaaatggacggcaccgaggagctgctggtaaagctgaacagagaagacctgctgcgcaaacagcgcactttcgacaatggaagcatcccccaccagattcacctgggcgaactgcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagattgagaaaatcctcacatttcggataccctactatgtaggccccctcgcccgcggaaattccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgaggaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttgataaaaatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgaatacttcacagtttacaacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcattcctgtctggagagcagaagaaagctatcgtggacctcctcttcaagaccaaccggaaagttaccgtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcgactctgttgaaatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaaaatcattaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacattgtcctcacccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaacttacgctcatctcttcgacgacaaagtgatgaaacagctcaagagacgccgatatacaggatgggggcggctgtcaagaaaactgat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05194_sequence/1
type: Sequence
persistent identity: BBa S05194 sequence
display id: BBa_S05194_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J63006_sequence/1
type: Sequence
persistent identity: BBa J63006 sequence
display id: BBa_J63006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05154_sequence/1
type: Sequence
persistent identity: BBa S05154 sequence
display id: BBa_S05154_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051105_sequence/1
type: Sequence
persistent identity: BBa K1051105 sequence
display id: BBa_K1051105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051701_sequence/1
type: Sequence
persistent identity: BBa K1051701 sequence
display id: BBa_K1051701_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1051003_sequence/1
type: Sequence
persistent identity: BBa K1051003 sequence
display id: BBa_K1051003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z