BBa_K1051301 1 BBa_K1051301 clb2 promoter (during G2 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z CTAGC ATCTC ATAGC ATGGG CATAGAGTTTCAGAAATTTTGCTCTTAATGGAAAATATAA CCTCTTTGGGGAAAAGAGAAATAGATCCTTTCTCAAAAATTTAGAAAGGAAAAAAAATCA AAATATAATAAAATAGATTTTTTTTAGGTTAATTTTTTGCCAATTTAGCTAGACTTTTGA AAAGATCCAAAAATTTACTAAAATTGAATCTTTCTGGTATTAATTTTGTCCTATACCTCA AGAAGTAAAGTATTCTCGAATTGATACTTCTGAAAAGCGAAAGAAATATAGCTAAAGGAT ACTGGTTATTTATTCTTTCTTTTTTTCTTTCTTTTTGATTGAGCCAGTGTATAATAAAAA CCTACTGCGAGCTATTCAATAAATTGACATAGAATATCTGTTGAAGCGGTTCTTTGATTG AGCATCTGTTTTCCTTTTGTATTTTTGTCCTTTTAGATTCTTTTAACGACATTGGAGAAA TTTTAATCATTGTATAAAAACTTCATTCGCTCGTTTGTCAGAAGTTTTTTTATAACTTGC TATTTCCTTCCAAGAAGCCTTTTATTGATTACCCCCTCTCTCTCTTCATTGATCTTATAG It is a promoter which promotes transciption in G2 of the yeast cell cycle .If you want to express your proteins in G2 of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 Not in stock false We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTAGCATCTCATAGCATGGG downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTACTATAAGATCAATGAAGAGA false Kequan Lin BBa_K1051902 1 BBa_K1051902 clb2+h2a2+RFP+PEST1+tyb-1 2013-09-24T11:00:00Z 2015-05-08T01:08:55Z clb2+h2a2+RFP+PEST1+tyb-1 false false _1358_ 0 15920 9 Not in stock false false Yang Zhou component2362457 1 BBa_K1051006 component2362454 1 BBa_K1051114 component2362456 1 BBa_K1051202 component2362455 1 BBa_K1051000 component2362453 1 BBa_K1051301 annotation2362454 1 BBa_K1051114 range2362454 1 1070 1477 annotation2362456 1 BBa_K1051202 range2362456 1 2167 2655 annotation2362457 1 BBa_K1051006 range2362457 1 2664 2694 annotation2362453 1 BBa_K1051301 range2362453 1 1 1063 annotation2362455 1 BBa_K1051000 range2362455 1 1484 2158 BBa_K1051006 1 BBa_K1051006 Saccharomycescerevisiae TBY-1 terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z Saccharomycescerevisiae Saccharomycescerevisiae terminator with stopcodon false false _1358_ 0 15912 9 It's complicated false stopcodon + terminator false Xiang LI BBa_K1051114 1 BBa_K1051114 H2A2;targeting peptide to nucleus 2013-07-11T11:00:00Z 2015-05-08T01:08:53Z Saccharomyces cerevisiae genomics sequence After added with 23 prefix and suffix, it can be used as a transporter to the nucleus of baking yeast. Also, when joining with different flourescent proteins, they can light the inner membrane using such proteins. false false _1358_ 0 15923 9 It's complicated false No. false Jinchun Zhang BBa_K1051202 1 BBa_K1051202 cln1-PEST 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z dbox dbox false false _1358_ 0 15924 9 In stock false dbox false wanling ZHOU BBa_K1051000 1 BBa_K1051000 Stop codon free RFP in RFC[23] standard 2013-07-01T11:00:00Z 2015-05-08T01:08:52Z engineered mutant of red fluorescent protein from Discosoma striata Red fluorescent protein with assembly standard 23 is engineered mutant of red fluorescent protein from Discosoma striata (coral). false false _1358_ 0 15912 9 It's complicated false You have to remove the termination codon before you use it false Xiang LI BBa_K1051902_sequence 1 ttttgttatccctgttgttgttgttttataagtcctaatctcgcttgaatatcgcccatacttaccctgttcttgaccgtctactaaggtgccttagggggactcaaacttaagatcgccttctacgcgttttatgctatgatcattagtgttgatgcatcggaagtttaaaaattaagtgcgagtgcattagcacagtggaattattagaatgaccactactccttctaatcaaacacgcggaaatagccgccaaaagacagattttattccaaatgcgggtaactatttgtataatatgtttacatattgagcccgtttaggaaagtgcaagttcaaggcactaatcaaaaaaggagatttgtaaatatagcgaccgaatcaggaaaaggtcaacaacgaagttcgcgatatggatgaacttcggtgcctgtccctcgaggaaatagcaaactcgttacagctagcatctcatagcatgggcatagagtttcagaaattttgctcttaatggaaaatataacctctttggggaaaagagaaatagatcctttctcaaaaatttagaaaggaaaaaaaatcaaaatataataaaatagattttttttaggttaattttttgccaatttagctagacttttgaaaagatccaaaaatttactaaaattgaatctttctggtattaattttgtcctatacctcaagaagtaaagtattctcgaattgatacttctgaaaagcgaaagaaatatagctaaaggatactggttatttattctttctttttttctttctttttgattgagccagtgtataataaaaacctactgcgagctattcaataaattgacatagaatatctgttgaagcggttctttgattgagcatctgttttccttttgtatttttgtccttttagattcttttaacgacattggagaaattttaatcattgtataaaaacttcattcgctcgtttgtcagaagtttttttataacttgctatttccttccaagaagccttttattgattaccccctctctctcttcattgatcttatagtactagatggccagaacaaagcaaacagcaagaaagtccactggtggtaaggccccaagaaagcaattagcttctaaggctgccagaaaatccgccccatctaccggtggtgttaagaagcctcacagatataagccaggtactgttgctttgagagaaatcagaagattccaaaaatctactgaactgttgatcagaaagttgcctttccaaagattggtcagagaaatcgctcaagatttcaagaccgacttgagatttcaatcttctgccatcggtgccttgcaagaatctgtcgaagcctacttagtctctttatttgaagataccaacttggctgccattcacgccaagcgtgtcactatccaaaagaaggatatcaagttggctagaagattaagaggtgaaagatcatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttactagagtctacaatagacctaaccagaaaaattgctgtcaattctcgttactttgaccaaaatgcctcttcatcatcagtttcttctccaagcacatattcttcgggaaccaattatactccaatgcgaaacttcagtgcacaatcagacaacagtgttttcagtactaccaacattgaccattcatcgccgatcacccctcacatgtacacttttaatcagtttaaaaacgaaagtgcttgtgacagtgccataagcgtaagcagtctacctaatcaaacccaaaatggtaacatgccattatcaagcaattatcagaatatgatgctagaagaaaggaataaagagaataggattcccaactcatcatccgctgaaataccacaacgtgccaaatttatgacgactggtatattccagaacacaggagaacttactaatagagcgtcttcgatatcgctatcgttaaggaaccacaatagctctcaactgtgatactagagtaatagtgtatgtgtataatatcatagttta BBa_K1051006_sequence 1 taatagtgtatgtgtataatatcatagttta BBa_K1051301_sequence 1 ttttgttatccctgttgttgttgttttataagtcctaatctcgcttgaatatcgcccatacttaccctgttcttgaccgtctactaaggtgccttagggggactcaaacttaagatcgccttctacgcgttttatgctatgatcattagtgttgatgcatcggaagtttaaaaattaagtgcgagtgcattagcacagtggaattattagaatgaccactactccttctaatcaaacacgcggaaatagccgccaaaagacagattttattccaaatgcgggtaactatttgtataatatgtttacatattgagcccgtttaggaaagtgcaagttcaaggcactaatcaaaaaaggagatttgtaaatatagcgaccgaatcaggaaaaggtcaacaacgaagttcgcgatatggatgaacttcggtgcctgtccctcgaggaaatagcaaactcgttacagctagcatctcatagcatgggcatagagtttcagaaattttgctcttaatggaaaatataacctctttggggaaaagagaaatagatcctttctcaaaaatttagaaaggaaaaaaaatcaaaatataataaaatagattttttttaggttaattttttgccaatttagctagacttttgaaaagatccaaaaatttactaaaattgaatctttctggtattaattttgtcctatacctcaagaagtaaagtattctcgaattgatacttctgaaaagcgaaagaaatatagctaaaggatactggttatttattctttctttttttctttctttttgattgagccagtgtataataaaaacctactgcgagctattcaataaattgacatagaatatctgttgaagcggttctttgattgagcatctgttttccttttgtatttttgtccttttagattcttttaacgacattggagaaattttaatcattgtataaaaacttcattcgctcgtttgtcagaagtttttttataacttgctatttccttccaagaagccttttattgattaccccctctctctcttcattgatcttatag BBa_K1051000_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgct BBa_K1051114_sequence 1 atggccagaacaaagcaaacagcaagaaagtccactggtggtaaggccccaagaaagcaattagcttctaaggctgccagaaaatccgccccatctaccggtggtgttaagaagcctcacagatataagccaggtactgttgctttgagagaaatcagaagattccaaaaatctactgaactgttgatcagaaagttgcctttccaaagattggtcagagaaatcgctcaagatttcaagaccgacttgagatttcaatcttctgccatcggtgccttgcaagaatctgtcgaagcctacttagtctctttatttgaagataccaacttggctgccattcacgccaagcgtgtcactatccaaaagaaggatatcaagttggctagaagattaagaggtgaaagatca BBa_K1051202_sequence 1 tctacaatagacctaaccagaaaaattgctgtcaattctcgttactttgaccaaaatgcctcttcatcatcagtttcttctccaagcacatattcttcgggaaccaattatactccaatgcgaaacttcagtgcacaatcagacaacagtgttttcagtactaccaacattgaccattcatcgccgatcacccctcacatgtacacttttaatcagtttaaaaacgaaagtgcttgtgacagtgccataagcgtaagcagtctacctaatcaaacccaaaatggtaacatgccattatcaagcaattatcagaatatgatgctagaagaaaggaataaagagaataggattcccaactcatcatccgctgaaataccacaacgtgccaaatttatgacgactggtatattccagaacacaggagaacttactaatagagcgtcttcgatatcgctatcgttaaggaaccacaatagctctcaactgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z