BBa_K1051700 1 BBa_K1051700 SRC1 Intron -4bp at 5 2013-06-28T11:00:00Z 2015-05-08T01:08:55Z GCAAGTGAGTACAATTTATTATCTTACAAAAATTATTAAACCTGTTATTATATGATAACAGTTTTAATTTTTTTTGAATTTTTTTTTGTTTTTGATTTATTTACTAACATCTTTTCCTAATTTTCTCTAG Intron from SRC1 can be spliced into two different forms by regulating the presence of Hub1 or Bud31 false false _1358_ 0 15920 9 In stock false should be used with Hub1 or Bud31 false Yang Zhou BBa_S05151 1 BBa_S05151 GFP+I700 2013-07-07T11:00:00Z 2015-05-08T01:14:50Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2332112 1 BBa_K1051700 component2332111 1 BBa_K1051003 annotation2332111 1 BBa_K1051003 range2332111 1 1 714 annotation2332112 1 BBa_K1051700 range2332112 1 723 851 BBa_K1051303 1 BBa_K1051303 clb6 promoter (during G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z TAACT AGGTG GTCCT TCCAT GAAAGTTTTTTTTTTTTGAAGATGTTCTTTTTTTTTTTTT GGGCCTCATTACAATGTAAGAGGTTATTATGTGTTACATGAGTAAAGCTAATTGACGCAA GCAGGTAATGAAGAAAGCCCTATATTACCGTATAGACAATGGGCGCGTCCCTAAAGAGGA TACCAAATAGCAGTTTTACGCGTACCACAAAATTTAACGACCAACGCGTATTCTTCGCGT TTTTTTTTTGCGACGGTAAACAAAAAAAGGGCCCTCTGCGGTAAAACAAGTGGGTCTACC ACAGGACACAGGACTTGGTTGTTTCTACTCTCCCTGTGTATCCTACAATTGGCAATTGGC AATTCGCAACCTGCGTTTAAATATTTACCGTTTTAGCACATTGATTACCTTTTTTGCTAG GGTTAAGTTTTTGCCTTTTTCTTATTAACATATATATAACTGCTTCATTTCATACAAGTG ACTTTTATCTTTTATTAAATAATAACAAGCTTCACTAATCTAGCGTTCATACAACATACA AATCAGTGTGTTGAAAATTATTATTCTCTGATATTCTCTCCCTCCTTTTAAATTTTTAAA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 It's complicated true We get the sequence which is actived by clb6 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTAACT AGGTG GTCCT TCCAT downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTAAAAATTTAAAAGGAGG false Kequan Lin BBa_K1051970 1 BBa_K1051970 clb6 P + D-box + GFP + I700 + TOM40 + T 2013-09-09T11:00:00Z 2015-05-08T01:08:55Z No clb5 Promoter + D-box + GFP + SRC1 Intron -4bp at 5' + Targeting Peptide to Mitochondria outer membrane + T false false _1358_ 0 15920 9 Not in stock false No false Yang Zhou component2367145 1 BBa_S05201 component2367152 1 BBa_K1051745 annotation2367152 1 BBa_K1051745 range2367152 1 753 1782 annotation2367145 1 BBa_S05201 range2367145 1 1 746 BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051105 1 BBa_K1051105 TOM40;targeting peptide to mitochondria outer membrane 2013-07-11T11:00:00Z 2015-05-08T01:08:53Z Saccharomyces cerevisiae genomics sequence After added with 23 prefix and suffix, it can be used as a transporter to the mitochondria inner membrane. Also, when joining with different flourescent proteins, they can light the inner membrane using such proteins. false false _1358_ 0 15923 9 It's complicated false ATG was added at start false Jinchun Zhang BBa_K1051745 1 BBa_K1051745 GFP+I700+TOM40+T 2013-09-12T11:00:00Z 2015-05-08T01:08:55Z No test false false _1358_ 0 15920 9 Not in stock false No false Yang Zhou component2339839 1 BBa_S05193 component2339840 1 BBa_K1051008 annotation2339839 1 BBa_S05193 range2339839 1 1 1004 annotation2339840 1 BBa_K1051008 range2339840 1 1013 1030 BBa_K1051003 1 BBa_K1051003 Stop codon free GFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit GFP without stop coding false false _1358_ 0 15912 9 In stock false add prefix suffix del stop coding false Xiang LI BBa_S05193 1 BBa_S05193 S05151:K1051105 2013-09-09T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2337786 1 BBa_K1051105 component2337785 1 BBa_S05151 annotation2337785 1 BBa_S05151 range2337785 1 1 851 annotation2337786 1 BBa_K1051105 range2337786 1 858 1004 BBa_K1051201 1 BBa_K1051201 D-BOX 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z 5' - AATTC GCGGCCGC T TCTAGA GTACAAAGGTTGGCCTTAAACAATGTAACAAATACGACA ACTAGT A GCGGCCG CTGCA- 3' 5???- G CGGCCGC T ACTAGT TGTCGTATTTGTTACATTGTTTAAGGCCAACCTTTGTAC TCTAGAA GCGGCCGC G-3??? D-BOX false false _1358_ 0 18073 9 In stock false D-BOX false wanling ZHOU BBa_S05201 1 BBa_S05201 K1051302:K1051201 2013-09-09T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2367141 1 BBa_K1051303 component2367142 1 BBa_K1051201 annotation2367142 1 BBa_K1051201 range2367142 1 708 746 annotation2367141 1 BBa_K1051303 range2367141 1 1 699 BBa_K1051970_sequence 1 acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagaggtacaaaggttggccttaaacaatgtaacaaatacgacatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagcggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttt BBa_K1051745_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagcggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttt BBa_S05201_sequence 1 acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagaggtacaaaggttggccttaaacaatgtaacaaatacgaca BBa_K1051303_sequence 1 acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaa BBa_S05151_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagcgg BBa_K1051201_sequence 1 gtacaaaggttggccttaaacaatgtaacaaatacgaca BBa_K1051008_sequence 1 taatttttattcattttt BBa_K1051003_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa BBa_K1051700_sequence 1 gtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagcgg BBa_K1051105_sequence 1 atgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattg BBa_S05193_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagcggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z