BBa_S05198 1 BBa_S05198 S05194:K1051008 2013-09-09T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2337815 1 BBa_S05194 component2337816 1 BBa_K1051008 annotation2337815 1 BBa_S05194 range2337815 1 1 1007 annotation2337816 1 BBa_K1051008 range2337816 1 1016 1033 BBa_K1051303 1 BBa_K1051303 clb6 promoter (during G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z TAACT AGGTG GTCCT TCCAT GAAAGTTTTTTTTTTTTGAAGATGTTCTTTTTTTTTTTTT GGGCCTCATTACAATGTAAGAGGTTATTATGTGTTACATGAGTAAAGCTAATTGACGCAA GCAGGTAATGAAGAAAGCCCTATATTACCGTATAGACAATGGGCGCGTCCCTAAAGAGGA TACCAAATAGCAGTTTTACGCGTACCACAAAATTTAACGACCAACGCGTATTCTTCGCGT TTTTTTTTTGCGACGGTAAACAAAAAAAGGGCCCTCTGCGGTAAAACAAGTGGGTCTACC ACAGGACACAGGACTTGGTTGTTTCTACTCTCCCTGTGTATCCTACAATTGGCAATTGGC AATTCGCAACCTGCGTTTAAATATTTACCGTTTTAGCACATTGATTACCTTTTTTGCTAG GGTTAAGTTTTTGCCTTTTTCTTATTAACATATATATAACTGCTTCATTTCATACAAGTG ACTTTTATCTTTTATTAAATAATAACAAGCTTCACTAATCTAGCGTTCATACAACATACA AATCAGTGTGTTGAAAATTATTATTCTCTGATATTCTCTCCCTCCTTTTAAATTTTTAAA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 It's complicated true We get the sequence which is actived by clb6 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTAACT AGGTG GTCCT TCCAT downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTAAAAATTTAAAAGGAGG false Kequan Lin BBa_K1051971 1 BBa_K1051971 clb6 P + D-box + GFP + I701 + TOM40 + T 2013-09-09T11:00:00Z 2015-05-08T01:08:55Z No clb5 Promoter + D-box + GFP + SRC1 Intron+GG + Targeting Peptide to Mitochondria outer membrane + T false false _1358_ 0 15920 9 Not in stock false No false Yang Zhou component2367155 1 BBa_S05201 component2367162 1 BBa_S05198 annotation2367155 1 BBa_S05201 range2367155 1 1 746 annotation2367162 1 BBa_S05198 range2367162 1 753 1785 BBa_S05201 1 BBa_S05201 K1051302:K1051201 2013-09-09T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2367142 1 BBa_K1051201 component2367141 1 BBa_K1051303 annotation2367142 1 BBa_K1051201 range2367142 1 708 746 annotation2367141 1 BBa_K1051303 range2367141 1 1 699 BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051105 1 BBa_K1051105 TOM40;targeting peptide to mitochondria outer membrane 2013-07-11T11:00:00Z 2015-05-08T01:08:53Z Saccharomyces cerevisiae genomics sequence After added with 23 prefix and suffix, it can be used as a transporter to the mitochondria inner membrane. Also, when joining with different flourescent proteins, they can light the inner membrane using such proteins. false false _1358_ 0 15923 9 It's complicated false ATG was added at start false Jinchun Zhang BBa_S05154 1 BBa_S05154 GFP+I701 2013-07-08T11:00:00Z 2015-05-08T01:14:50Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2332115 1 BBa_K1051003 component2332116 1 BBa_K1051701 annotation2332115 1 BBa_K1051003 range2332115 1 1 714 annotation2332116 1 BBa_K1051701 range2332116 1 723 854 BBa_K1051003 1 BBa_K1051003 Stop codon free GFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit GFP without stop coding false false _1358_ 0 15912 9 In stock false add prefix suffix del stop coding false Xiang LI BBa_K1051701 1 BBa_K1051701 SRC1 Intron+GG 2013-06-29T11:00:00Z 2015-05-08T01:08:55Z From SRC1 Intron from SRC1 and is added GG to form a Ser codon false false _1358_ 0 15920 9 In stock false no false Yang Zhou BBa_K1051201 1 BBa_K1051201 D-BOX 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z 5' - AATTC GCGGCCGC T TCTAGA GTACAAAGGTTGGCCTTAAACAATGTAACAAATACGACA ACTAGT A GCGGCCG CTGCA- 3' 5???- G CGGCCGC T ACTAGT TGTCGTATTTGTTACATTGTTTAAGGCCAACCTTTGTAC TCTAGAA GCGGCCGC G-3??? D-BOX false false _1358_ 0 18073 9 In stock false D-BOX false wanling ZHOU BBa_S05194 1 BBa_S05194 S05154:K1051105 2013-09-09T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2337792 1 BBa_K1051105 component2337791 1 BBa_S05154 annotation2337791 1 BBa_S05154 range2337791 1 1 854 annotation2337792 1 BBa_K1051105 range2337792 1 861 1007 BBa_K1051971_sequence 1 acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagaggtacaaaggttggccttaaacaatgtaacaaatacgacatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttt BBa_S05201_sequence 1 acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagaggtacaaaggttggccttaaacaatgtaacaaatacgaca BBa_K1051303_sequence 1 acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaa BBa_K1051201_sequence 1 gtacaaaggttggccttaaacaatgtaacaaatacgaca BBa_K1051008_sequence 1 taatttttattcattttt BBa_S05198_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttt BBa_S05154_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctaggg BBa_K1051003_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa BBa_K1051105_sequence 1 atgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattg BBa_S05194_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctagggtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattg BBa_K1051701_sequence 1 gcaagtgagtacaatttattatcttacaaaaattattaaacctgttattatatgataacagttttaattttttttgaatttttttttgtttttgatttatttactaacatcttttcctaattttctctaggg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z