BBa_K1051974 1 BBa_K1051974 clb6 P + D-box + GFP + MER2 Intron + TOM40 + T 2013-09-20T11:00:00Z 2015-05-08T01:08:55Z No clb5 Promoter + D-box + GFP + MER2 Intron + Targeting Peptide to Mitochondria outer membrane + T false false _1358_ 0 15920 9 Not in stock false No false Yang Zhou component2367188 1 BBa_K1051755 component2367181 1 BBa_K1051303 annotation2367181 1 BBa_K1051303 range2367181 1 1 699 annotation2367188 1 BBa_K1051755 range2367188 1 706 1686 BBa_K1051303 1 BBa_K1051303 clb6 promoter (during G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z TAACT AGGTG GTCCT TCCAT GAAAGTTTTTTTTTTTTGAAGATGTTCTTTTTTTTTTTTT GGGCCTCATTACAATGTAAGAGGTTATTATGTGTTACATGAGTAAAGCTAATTGACGCAA GCAGGTAATGAAGAAAGCCCTATATTACCGTATAGACAATGGGCGCGTCCCTAAAGAGGA TACCAAATAGCAGTTTTACGCGTACCACAAAATTTAACGACCAACGCGTATTCTTCGCGT TTTTTTTTTGCGACGGTAAACAAAAAAAGGGCCCTCTGCGGTAAAACAAGTGGGTCTACC ACAGGACACAGGACTTGGTTGTTTCTACTCTCCCTGTGTATCCTACAATTGGCAATTGGC AATTCGCAACCTGCGTTTAAATATTTACCGTTTTAGCACATTGATTACCTTTTTTGCTAG GGTTAAGTTTTTGCCTTTTTCTTATTAACATATATATAACTGCTTCATTTCATACAAGTG ACTTTTATCTTTTATTAAATAATAACAAGCTTCACTAATCTAGCGTTCATACAACATACA AATCAGTGTGTTGAAAATTATTATTCTCTGATATTCTCTCCCTCCTTTTAAATTTTTAAA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 It's complicated true We get the sequence which is actived by clb6 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTAACT AGGTG GTCCT TCCAT downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTAAAAATTTAAAAGGAGG false Kequan Lin BBa_S05219 1 BBa_S05219 K1051003:K1051704 2013-09-13T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2341358 1 BBa_K1051003 component2341359 1 BBa_K1051704 annotation2341358 1 BBa_K1051003 range2341358 1 1 714 annotation2341359 1 BBa_K1051704 range2341359 1 723 802 BBa_K1051755 1 BBa_K1051755 GFP + MER2 Intron + TOM40 + T 2013-09-20T11:00:00Z 2015-05-08T01:08:55Z NO GFP + MER2 Intron + Targeting Peptide to Mitochondria outer membrane + T false false _1358_ 0 15920 9 Not in stock false NO false Yang Zhou component2357337 1 BBa_S05236 component2357338 1 BBa_K1051008 annotation2357337 1 BBa_S05236 range2357337 1 1 955 annotation2357338 1 BBa_K1051008 range2357338 1 964 981 BBa_K1051008 1 BBa_K1051008 S. cerevisiae CAA terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z S. cerevisiae S. cerevisiae terminator with stopcodon false false _1358_ 0 15912 9 In stock false stopcodon + terminator false Xiang LI BBa_K1051105 1 BBa_K1051105 TOM40;targeting peptide to mitochondria outer membrane 2013-07-11T11:00:00Z 2015-05-08T01:08:53Z Saccharomyces cerevisiae genomics sequence After added with 23 prefix and suffix, it can be used as a transporter to the mitochondria inner membrane. Also, when joining with different flourescent proteins, they can light the inner membrane using such proteins. false false _1358_ 0 15923 9 It's complicated false ATG was added at start false Jinchun Zhang BBa_K1051704 1 BBa_K1051704 Mer2 Intron 2013-06-29T11:00:00Z 2015-05-08T01:08:55Z From MER2 Intron from MER2. Can be splice into two different forms(spliced or unspliced) by regulating the presence of Mer1. false false _1358_ 0 15920 9 Not in stock false No false Yang Zhou BBa_K1051003 1 BBa_K1051003 Stop codon free GFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit GFP without stop coding false false _1358_ 0 15912 9 In stock false add prefix suffix del stop coding false Xiang LI BBa_S05236 1 BBa_S05236 K1051003:K1051704 2013-09-20T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 15920 9 Not in stock false false Yang Zhou component2357332 1 BBa_K1051105 component2357331 1 BBa_S05219 annotation2357332 1 BBa_K1051105 range2357332 1 809 955 annotation2357331 1 BBa_S05219 range2357331 1 1 802 BBa_S05219_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggttcgtaccaacacagtgcataccctcaagttttttattcattttcttccaaaacacatttactaacaactgtagtatag BBa_K1051303_sequence 1 acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaa BBa_K1051755_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggttcgtaccaacacagtgcataccctcaagttttttattcattttcttccaaaacacatttactaacaactgtagtatagtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttt BBa_K1051008_sequence 1 taatttttattcattttt BBa_K1051003_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa BBa_S05236_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggttcgtaccaacacagtgcataccctcaagttttttattcattttcttccaaaacacatttactaacaactgtagtatagtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattg BBa_K1051105_sequence 1 atgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattg BBa_K1051974_sequence 1 acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagaggttcgtaccaacacagtgcataccctcaagttttttattcattttcttccaaaacacatttactaacaactgtagtatagtactagatgttttgcggtgaaatcgatcatttcaagaacgataccaagattggttgcggtctacaatttgaaactgctggtaatcaagaattactaatgttacaacaaggtttagacgcagatggtaacccattgcaagctcttcctcaattgtactagagtaatttttattcattttt BBa_K1051704_sequence 1 gttcgtaccaacacagtgcataccctcaagttttttattcattttcttccaaaacacatttactaacaactgtagtatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z