LOCUS       BBa_K1053106            2163 bp    DNA     linear       29-Nov-2024
DEFINITION  Pconst.(H)-RBS-FA-linker-MS2-RBS-FB-linker-PP7-double terminator
ACCESSION   BBa_K1053106
VERSION     BBa_K1053106.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             64..1346
                     /label=BBa_K1053103
     misc_feature    1359..1362
                     /label=conserved
     RBS             1355..1366
                     /label=BBa_B0034
     CDS             1373..2026
                     /label=BBa_K1053104
     stem_loop       2046..2089
                     /label=stem_loop
     misc_feature    2035..2114
                     /label=BBa_B0010
     terminator      2035..2114
                     /label=BBa_B0010
     stem_loop       2130..2149
                     /label=T7 TE
     misc_feature    2156..2156
                     /label=stop
     polyA_site      2150..2163
                     /label=polya
     misc_feature    2123..2163
                     /label=BBa_B0012
     terminator      2123..2163
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gagtggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      121 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      181 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      241 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      301 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      361 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      421 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      481 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagggag
      541 gaggaggatc aggcggtggt ggctctatgg catccaactt cacacagttc gttttggttg
      601 ataacggcgg taccggcgac gttaccgtgg cgccaagcaa ttttgccaac ggcgtggcgg
      661 aatggattag cagcaacagc cgctcgcagg cttacaaggt gacctgctcg gtgcgccaga
      721 gctctgcaca gaatcgtaag tatacgatta aggttgaagt accaaaagtg gcaacccaaa
      781 ccgtcggcgg ggtggaactg ccggtcgcgg cgtggcgcag ctatctgaat atggaactga
      841 ccatcccgat ttttgcgaca aatagtgatt gtgagctgat tgtaaaagcc atgcaagggc
      901 ttttgaaaga cggtaacccg attccgtcgg caattgcggc caacagcggt atctatgcga
      961 acttcaccca atttgttctg gtggacaatg gaggtacggg tgatgtgacc gtggctccca
     1021 gcaattttgc gaacggagtg gcggaatgga tttcatccaa ttcacgttcg caagcgtata
     1081 aagtaacgtg ctcagtgcgt cagagttcag cacagaaccg taaatacacg attaaagtcg
     1141 aagtaccgaa agttgcaact cagaccgtag gcggcgtcga gctgccggtt gccgcgtggc
     1201 gcagttacct gaatatggag ttaactattc ctatctttgc cactaattct gattgtgaac
     1261 ttattgtcaa agccatgcag ggcttactca aagatggcaa cccgatccct tctgccatcg
     1321 ccgccaacag tggcatctac taataatact agagaaagag gagaaatact agatgggcaa
     1381 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1441 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1501 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1561 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1621 gggaggagga ggatcaggcg gtggtggctc tatgtccaaa accatcgttc tttcggtcgg
     1681 cgaggctact cgcactctga ctgagatcca gtccaccgca gaccgtcaga tcttcgaaga
     1741 gaaggtcggg cctctggtgg gtcggctgcg cctcacggct tcgctccgtc aaaacggagc
     1801 caagaccgcg tatcgcgtca acctaaaact ggatcaggcg gacgtcgttg attccggact
     1861 tccgaaagtg cgctacactc aggtatggtc gcacgacgtg acaatcgttg cgaatagcac
     1921 cgaggcctcg cgcaaatcgt tgtacgattt gaccaagtcc ctcgtcgcga cctcgcaggt
     1981 cgaagatctt gtcgtcaacc ttgtgccgct gggccgttga taataatact agagccaggc
     2041 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2101 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2161 ata
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