BBa_K1053104 1 BBa_K1053104 FB-linker-PP7 2013-09-11T11:00:00Z 2015-05-08T01:08:56Z K738005 Split GFP C-terminal domain fused with PP7 protein false false _1360_ 0 18170 9 It's complicated false Split GFP C-terminal domain fused with PP7 protein false Eri Takagi BBa_K1053103 1 BBa_K1053103 FA-linker-MS2 2013-09-09T11:00:00Z 2015-05-08T01:08:56Z K738004 Split GFP N-terminal domain fused with MS2 protein false false _1360_ 0 18170 9 In stock false Split GFP N-terminal domain fused with MS2 protein false Eri Takagi BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1053106 1 BBa_K1053106 Pconst.(H)-RBS-FA-linker-MS2-RBS-FB-linker-PP7-double terminator 2013-10-10T11:00:00Z 2015-05-08T01:08:56Z We used the synthesized sequences of split GFP fused with MS2 and PP7 (FA-linker-MS2, FB-linker-PP7) respectively, reference of BioBrick part BBa_K738004 (FA-linker-MS2) and BBa_K738005 (FB-linker-PP7) (ZJU-China 2012). Split GFP N-terminal domain (FA) is bound to MS2 through linker. Split GFP is consisted of two half GFP; FA (N-terminal domain) and FB (C-terminal domain) between amino acid residue 158 and 159. Either FA or FB is not able to emit fluorescence. When FA and FB come closer, the fragments associate and restore fluorescence. Also, MS2 is the single strand RNA phage coat protein which is bound specifically by MS2 aptamer. false false _1360_ 0 18170 9 Not in stock false The synthesized three-part sequence for FA-linker-MS2 was ligated by overlap PCR. false Eri Takagi component2369516 1 BBa_B0012 component2369510 1 BBa_K1053103 component2369507 1 BBa_J23100 component2369512 1 BBa_B0034 component2369514 1 BBa_B0010 component2369513 1 BBa_K1053104 component2369509 1 BBa_B0034 annotation2369510 1 BBa_K1053103 range2369510 1 64 1346 annotation2369513 1 BBa_K1053104 range2369513 1 1373 2026 annotation2369514 1 BBa_B0010 range2369514 1 2035 2114 annotation2369507 1 BBa_J23100 range2369507 1 1 35 annotation2369509 1 BBa_B0034 range2369509 1 44 55 annotation2369512 1 BBa_B0034 range2369512 1 1355 1366 annotation2369516 1 BBa_B0012 range2369516 1 2123 2163 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1053103_sequence 1 tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagggaggaggaggatcaggcggtggtggctctatggcatccaacttcacacagttcgttttggttgataacggcggtaccggcgacgttaccgtggcgccaagcaattttgccaacggcgtggcggaatggattagcagcaacagccgctcgcaggcttacaaggtgacctgctcggtgcgccagagctctgcacagaatcgtaagtatacgattaaggttgaagtaccaaaagtggcaacccaaaccgtcggcggggtggaactgccggtcgcggcgtggcgcagctatctgaatatggaactgaccatcccgatttttgcgacaaatagtgattgtgagctgattgtaaaagccatgcaagggcttttgaaagacggtaacccgattccgtcggcaattgcggccaacagcggtatctatgcgaacttcacccaatttgttctggtggacaatggaggtacgggtgatgtgaccgtggctcccagcaattttgcgaacggagtggcggaatggatttcatccaattcacgttcgcaagcgtataaagtaacgtgctcagtgcgtcagagttcagcacagaaccgtaaatacacgattaaagtcgaagtaccgaaagttgcaactcagaccgtaggcggcgtcgagctgccggttgccgcgtggcgcagttacctgaatatggagttaactattcctatctttgccactaattctgattgtgaacttattgtcaaagccatgcagggcttactcaaagatggcaacccgatcccttctgccatcgccgccaacagtggcatctactaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1053104_sequence 1 atgggcaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagggaggaggaggatcaggcggtggtggctctatgtccaaaaccatcgttctttcggtcggcgaggctactcgcactctgactgagatccagtccaccgcagaccgtcagatcttcgaagagaaggtcgggcctctggtgggtcggctgcgcctcacggcttcgctccgtcaaaacggagccaagaccgcgtatcgcgtcaacctaaaactggatcaggcggacgtcgttgattccggacttccgaaagtgcgctacactcaggtatggtcgcacgacgtgacaatcgttgcgaatagcaccgaggcctcgcgcaaatcgttgtacgatttgaccaagtccctcgtcgcgacctcgcaggtcgaagatcttgtcgtcaaccttgtgccgctgggccgttgataataa BBa_K1053106_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagagtggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagggaggaggaggatcaggcggtggtggctctatggcatccaacttcacacagttcgttttggttgataacggcggtaccggcgacgttaccgtggcgccaagcaattttgccaacggcgtggcggaatggattagcagcaacagccgctcgcaggcttacaaggtgacctgctcggtgcgccagagctctgcacagaatcgtaagtatacgattaaggttgaagtaccaaaagtggcaacccaaaccgtcggcggggtggaactgccggtcgcggcgtggcgcagctatctgaatatggaactgaccatcccgatttttgcgacaaatagtgattgtgagctgattgtaaaagccatgcaagggcttttgaaagacggtaacccgattccgtcggcaattgcggccaacagcggtatctatgcgaacttcacccaatttgttctggtggacaatggaggtacgggtgatgtgaccgtggctcccagcaattttgcgaacggagtggcggaatggatttcatccaattcacgttcgcaagcgtataaagtaacgtgctcagtgcgtcagagttcagcacagaaccgtaaatacacgattaaagtcgaagtaccgaaagttgcaactcagaccgtaggcggcgtcgagctgccggttgccgcgtggcgcagttacctgaatatggagttaactattcctatctttgccactaattctgattgtgaacttattgtcaaagccatgcagggcttactcaaagatggcaacccgatcccttctgccatcgccgccaacagtggcatctactaataatactagagaaagaggagaaatactagatgggcaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagggaggaggaggatcaggcggtggtggctctatgtccaaaaccatcgttctttcggtcggcgaggctactcgcactctgactgagatccagtccaccgcagaccgtcagatcttcgaagagaaggtcgggcctctggtgggtcggctgcgcctcacggcttcgctccgtcaaaacggagccaagaccgcgtatcgcgtcaacctaaaactggatcaggcggacgtcgttgattccggacttccgaaagtgcgctacactcaggtatggtcgcacgacgtgacaatcgttgcgaatagcaccgaggcctcgcgcaaatcgttgtacgatttgaccaagtccctcgtcgcgacctcgcaggtcgaagatcttgtcgtcaaccttgtgccgctgggccgttgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z