LOCUS BBa_K1053303 3421 bp DNA linear 16-Dec-2024 DEFINITION PlacO-1-RBS-tetR-DT-PcI-RBS-lacI-DT-PtetO-1-RBS-cI-DT ACCESSION BBa_K1053303 VERSION BBa_K1053303.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 230..846 /label=tetR misc_feature 847..880 /label=SsrA misc_feature 887..911 /label=Help:Barcodes CDS 227..911 /label=BBa_C0040 stem_loop 931..974 /label=stem_loop misc_feature 920..999 /label=BBa_B0010 terminator 920..999 /label=BBa_B0010 stem_loop 1015..1034 /label=T7 TE misc_feature 1041..1041 /label=stop polyA_site 1035..1048 /label=polya misc_feature 1008..1048 /label=BBa_B0012 terminator 1008..1048 /label=BBa_B0012 terminator 920..1048 /label=BBa_B0015 promoter 1094..1099 /label=-10 promoter 1071..1076 /label=-35 misc_feature 1081..1097 /label=OR1 misc_feature 1057..1073 /label=OR2 misc_feature 1057..1105 /label=BBa_R0051 promoter 1057..1105 /label=BBa_R0051 misc_feature 1118..1121 /label=conserved RBS 1114..1125 /label=BBa_B0034 misc_feature 2221..2259 /label=LVA CDS 1132..2259 /label=lacI-LVA misc_feature 1132..2259 /label=BBa_C0012 misc_feature 2260..2284 /label=Help:Barcodes CDS 1132..2259 /label=BBa_C0012 stem_loop 2304..2347 /label=stem_loop misc_feature 2293..2372 /label=BBa_B0010 terminator 2293..2372 /label=BBa_B0010 stem_loop 2388..2407 /label=T7 TE misc_feature 2414..2414 /label=stop polyA_site 2408..2421 /label=polya misc_feature 2381..2421 /label=BBa_B0012 terminator 2381..2421 /label=BBa_B0012 terminator 2293..2421 /label=BBa_B0015 misc_feature 2430..2448 /label=TetR 1 misc_feature 2430..2483 /label=BBa_R0040 promoter 2449..2454 /label=-35 misc_feature 2455..2473 /label=TetR 2 promoter 2472..2477 /label=-10 promoter 2430..2483 /label=BBa_R0040 misc_feature 2496..2499 /label=conserved RBS 2492..2503 /label=BBa_B0034 CDS 2513..3220 /label=cI lambda misc_feature 3221..3253 /label=LVA misc_feature 3260..3284 /label=Help:Barcodes CDS 2510..3284 /label=BBa_C0051 stem_loop 3304..3347 /label=stem_loop misc_feature 3293..3372 /label=BBa_B0010 terminator 3293..3372 /label=BBa_B0010 stem_loop 3388..3407 /label=T7 TE misc_feature 3414..3414 /label=stop polyA_site 3408..3421 /label=polya misc_feature 3381..3421 /label=BBa_B0012 terminator 3381..3421 /label=BBa_B0012 terminator 3293..3421 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga 241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt 301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt 361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac 421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag 481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc 541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt 601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt 661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag 721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc 781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag 841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 901 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1021 ccttcgggtg ggcctttctg cgtttatata ctagagtaac accgtgcgtg ttgactattt 1081 tacctctggc ggtgataatg gttgctacta gagaaagagg agaaatacta gatggtgaat 1141 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt 1201 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg 1261 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag 1321 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc 1381 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa 1441 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt 1501 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc 1561 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt 1621 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag 1681 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc 1741 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg 1801 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact 1861 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc 1921 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca 1981 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc 2041 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc 2101 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc 2161 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 2221 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 2281 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2341 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2401 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta 2461 tcagtgatag agatactgag cactactaga gaaagaggag aaatactaga tgagcacaaa 2521 aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga 2581 aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg 2641 gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc 2701 attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga 2761 aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta 2821 ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa cctttaccaa 2881 aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct 2941 tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg 3001 aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag 3061 acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt 3121 acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg 3181 gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta 3241 cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat 3301 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 3361 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 3421 a //