BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1054005 1 BBa_K1054005 LrgB+Double Terminator 2013-09-09T11:00:00Z 2015-05-08T01:08:56Z false false _9_ 0 15789 9 It's complicated false false Jiasheng Wang component2346812 1 BBa_B0015 component2346805 1 BBa_K1054003 annotation2346805 1 BBa_K1054003 range2346805 1 1 696 annotation2346812 1 BBa_B0015 range2346812 1 705 833 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 BBa_K1054003 1 BBa_K1054003 lrgB antiholin factor 2013-09-09T11:00:00Z 2015-05-08T01:08:56Z Bacillus subtilis Inhibit cell lysis caused by holin (BBa_K1054002) false false _1361_ 0 15789 9 It's complicated false No false Jiasheng Wang annotation2338528 1 start range2338528 1 1 3 annotation2338529 1 stop range2338529 1 694 696 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1054005_sequence 1 atggaaagcacaatgagtccttattttgggattgtcgtatcacttgctgcattcggcatcggcacctttttatttaagaaaacgaaaggcttcttccttttcactcctttgtttgttgccatggtgctggggatcgcatttttaaaaatcggcggtttttcctacgcggattataataacgggggagagatcattaagttttttcttgaaccggcaacgatcgcatttgcaattcctttgtacaaacaaagagataagcttaaaaagtactggtggcaaatcatggcctccattattgccggctctatctgctctgtcacgattgtttatctgctcgctaaaggcattcatctcgattcggccgtaatgaaaagcatgcttccgcaggcggctacaacggcaattgcgcttcctttatcaaaaggcatcgggggcatcagcgacatcacagcctttgcggtcatctttaacgctgtcatcgtatatgcgctcggagcattgttcttgaaggtgtttaaagtgaaaaatccgatttctaaaggattggctcttggaacatccggacatgcacttggtgtagcggtcgggattgagatgggagaagttgaagctgcgatggcaagtattgctgttgtcgtagtaggagtggtaacggttctggtcatccctgtgttcgtgcagctgatcggaggataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1054003_sequence 1 atggaaagcacaatgagtccttattttgggattgtcgtatcacttgctgcattcggcatcggcacctttttatttaagaaaacgaaaggcttcttccttttcactcctttgtttgttgccatggtgctggggatcgcatttttaaaaatcggcggtttttcctacgcggattataataacgggggagagatcattaagttttttcttgaaccggcaacgatcgcatttgcaattcctttgtacaaacaaagagataagcttaaaaagtactggtggcaaatcatggcctccattattgccggctctatctgctctgtcacgattgtttatctgctcgctaaaggcattcatctcgattcggccgtaatgaaaagcatgcttccgcaggcggctacaacggcaattgcgcttcctttatcaaaaggcatcgggggcatcagcgacatcacagcctttgcggtcatctttaacgctgtcatcgtatatgcgctcggagcattgttcttgaaggtgtttaaagtgaaaaatccgatttctaaaggattggctcttggaacatccggacatgcacttggtgtagcggtcgggattgagatgggagaagttgaagctgcgatggcaagtattgctgttgtcgtagtaggagtggtaacggttctggtcatccctgtgttcgtgcagctgatcggaggataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z