LOCUS       BBa_K1054013            2490 bp    DNA     linear       25-Nov-2024
DEFINITION  pLuxR+CheZ+RFP
ACCESSION   BBa_K1054013
VERSION     BBa_K1054013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1043
                     /label=BBa_J09855
     RBS             1051..1063
                     /label=RBS
     CDS             1064..1708
                     /label=CheZ
     misc_feature    1064..1066
                     /label=start codon
     misc_feature    1703..1708
                     /label=stop codon
     CDS             1..1708
                     /label=BBa_K1054012
     RBS             1717..1731
                     /label=RBS-1\Strong
     RBS             1724..1728
                     /label=RBS
     misc_feature    1717..1731
                     /label=BBa_B0030
     RBS             1717..1731
                     /label=BBa_B0030
     CDS             1738..2412
                     /label=mrfp1
     misc_feature    2419..2443
                     /label=Help:Barcodes
     CDS             1738..2443
                     /label=BBa_E1010
     misc_feature    2452..2490
                     /label=B1006
     stem_loop       2461..2482
                     /label=modified thr terminator
     polyA_site      2483..2490
                     /label=PolyA
     polyA_site      2452..2460
                     /label=PolyA
     terminator      2452..2490
                     /label=BBa_B1006
     misc_feature    1717..2490
                     /label=BBa_K081014
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag aaaagaggag aaaatgcaac catcaatcaa
     1081 acctgctgac gagcattcag ctggcgatat cattgtgcgc atcggcagcc tgacgcgtat
     1141 gctgcgcgac agtttgcggg aactggggct ggatcaggcc attgccgaag cggcggaagc
     1201 catccccgat gcgcgcgatc gtttgtacta tgttgtgcag atgaccgccc aggctgcgga
     1261 gcgggcgctg aacagtgttg aggcgtcaca accgcatcag gatcaaatgg agaaatcagc
     1321 aaaagcgtta acccaacgtt gggatgactg gtttgccgat ccgattgacc ttgccgacgc
     1381 ccgtgaactg gtaacagata cacgacaatt tctggcagat gtacccgcgc ataccagctt
     1441 tactaacgcg caactgctgg aaatcatgat ggcgcaggat tttcaggatc tcaccgggca
     1501 ggtcattaag cggatgatgg atgtcattca ggagatcgaa cgccagttgc tgatggtgct
     1561 gttggaaaac atcccggaac aggagtcgcg tccaaaacgt gaaaaccaga gtttgcttaa
     1621 tggacctcag gtcgatacca gcaaagccgg tgtggtagcc agtcaggatc aggtggacga
     1681 tttgttggat agtcttggat tttaataata ctagagatta aagaggagaa atactagatg
     1741 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
     1801 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
     1861 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
     1921 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
     1981 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
     2041 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
     2101 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
     2161 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc
     2221 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac
     2281 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc
     2341 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac
     2401 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gaaaaaaaaa
     2461 ccccgcccct gacagggcgg ggtttttttt
//