##gff-version 3
##sequence-region BBa_K1054013 1 2490
BBa_K1054013	.	engineered_region	1717	2490	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K1054013	.	CDS	1738	2443	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K1054013	.	CDS	1738	2412	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1054013	.	engineered_tag	2419	2443	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1054013	.	ribosome_entry_site	1717	1731	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K1054013	.	ribosome_entry_site	1717	1731	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1054013	.	engineered_region	1717	1731	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1054013	.	ribosome_entry_site	1724	1728	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1054013	.	terminator	2452	2490	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K1054013	.	polyA_site	2452	2460	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1054013	.	stem_loop	2461	2482	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1054013	.	polyA_site	2483	2490	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1054013	.	engineered_region	2452	2490	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1054013	.	CDS	1	1708	.	+	0	ID=BBa_K1054012;Name=BBa_K1054012
BBa_K1054013	.	ribosome_entry_site	1051	1063	.	+	0	ID=annotation2342076;Name=RBS;Parent=BBa_K1054012
BBa_K1054013	.	engineered_region	1	1043	.	+	0	ID=annotation2342075;Name=BBa_J09855;Parent=BBa_K1054012
BBa_K1054013	.	CDS	1064	1708	.	+	0	ID=annotation2342077;Name=CheZ;Parent=BBa_K1054012
BBa_K1054013	.	stop_codon	1703	1708	.	+	0	ID=annotation2342079;Name=stop codon;Parent=BBa_K1054012
BBa_K1054013	.	start_codon	1064	1066	.	+	0	ID=annotation2342078;Name=start codon;Parent=BBa_K1054012
>BBa_K1054013
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagaaaagaggagaaaatgcaaccatcaatcaaacctgctgacgagcattcagctggcg
atatcattgtgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgc
cgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgg
gcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttggg
atgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgt
acccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcag
gtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaac
aggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagc
cagtcaggatcaggtggacgatttgttggatagtcttggattttaataatactagagattaaagaggagaaatactaga
tggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactag
agaaaaaaaaaccccgcccctgacagggcggggtttttttt