BBa_K1059001 1 BBa_K1059001 GFP-LVA under J23101 control 2013-09-16T11:00:00Z 2015-05-08T01:08:57Z The GFP-LVA sequence is from part 145280, but we change the RBS. This device consists of a GFP with LVA tag under the control of J23101 promoter. false false _1367_ 0 15654 9 In stock false The GFP-LVA is from part K145280, but it is reported that the RBS is so weak that it cannot work well, so we have to change the RBS to B0035 by PCR. false Yu Wang annotation2352564 1 start range2352564 1 66 78 annotation2352566 1 stop codon range2352566 1 818 826 annotation2352562 1 J23101 range2352562 1 1 35 annotation2352565 1 GFP-LVA range2352565 1 66 824 annotation2352567 1 B0015 range2352567 1 833 961 annotation2352563 1 B0035 range2352563 1 46 65 BBa_K1059001_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagatattaaagaggagaataaataatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z