BBa_K1059002
1
BBa_K1059002
GFP-LVA under B0035 control
2013-09-16T11:00:00Z
2015-05-08T01:08:57Z
The GFP-LVA sequence is from part K145280, we change the RBS B0032 to B0035 and standardize it by PCR .
This device consists of a GFP with LVA tag under the control of B0035. And at the end of the part, there???s a terminator named B0015.
false
false
_1367_
0
15654
9
In stock
false
The GFP-LVA is from part K145280, but it is reported that the RBS is so weak that it cannot work well, so we have to change the RBS to B0035 by PCR.
false
Yu Wang
annotation2352610
1
rbs
range2352610
1
4
23
annotation2352613
1
stop codon
range2352613
1
774
779
annotation2352612
1
start
range2352612
1
24
26
annotation2352611
1
GFP-LVA
range2352611
1
24
779
annotation2352614
1
B0015
range2352614
1
791
919
BBa_K1059002_sequence
1
gatattaaagaggagaataaataatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z