BBa_K1059002 1 BBa_K1059002 GFP-LVA under B0035 control 2013-09-16T11:00:00Z 2015-05-08T01:08:57Z The GFP-LVA sequence is from part K145280, we change the RBS B0032 to B0035 and standardize it by PCR . This device consists of a GFP with LVA tag under the control of B0035. And at the end of the part, there???s a terminator named B0015. false false _1367_ 0 15654 9 In stock false The GFP-LVA is from part K145280, but it is reported that the RBS is so weak that it cannot work well, so we have to change the RBS to B0035 by PCR. false Yu Wang annotation2352611 1 GFP-LVA range2352611 1 24 779 annotation2352614 1 B0015 range2352614 1 791 919 annotation2352612 1 start range2352612 1 24 26 annotation2352613 1 stop codon range2352613 1 774 779 annotation2352610 1 rbs range2352610 1 4 23 BBa_K1059002_sequence 1 gatattaaagaggagaataaataatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z