BBa_K1059014 1 BBa_K1059014 RBS B0032+mamK coding squence 2013-09-15T11:00:00Z 2015-05-08T01:08:57Z It comes from Magnetospirillum magneticum AMB-1 bacteria strain.We firstly design primers to get this gene squence by PCR from genome, and then use another primer to add RBS J23106, prefix and sufix onto it. mamK is a protein coding squence from Magnetospirillum magneticum AMB-1 bacteria strain, mamAB gene cluster. It will guide the construction of MMB with mamI, mamB, mamL, mamQ, which we use to construct the intracellular compartment. false false _1367_ 0 15604 9 It's complicated false Because there is a PstI cut site, so we process point mutation by over-lap PCR.synthetic biology proposes standard prefix and sufix, so we add prefix and sufix by PCR before we ligease it onto plasmid PSB1C3. false Wenjun Wang annotation2347126 1 BBa_B0032 range2347126 1 1 13 annotation2347127 1 RBS range2347127 1 7 10 annotation2347125 1 RBS-3\Weak range2347125 1 1 13 BBa_K1059014_sequence 1 tcacacaggaaagatgagtgaaggtgaaggccaggccaagaacagattgttccttggcatcgaccttgggacttcccatactgcggtgatgtcgagccggggcaaaaagttcctgctgaagtcggtggttggatacccgaaggacgtcatcggtttgaagctgctcggccgcccctatgtggtcggtgacgaggctttcgagatgcgctcctacctggacatccgctatccgctccaggacggcgtgctgagcgagatcagcgaccgcgacatcgaagtggcgcggcatctgctgacccatgtggtgaagtcggccgagcccggccccaacgacgagatctgcgccgtcatcggcgtgccggcgcgggcgtcggcggccaacaaggcgctgctgctcaagatggctcaggaggtggttcataccgccctagtggtgtccgaaccgttcatggtcggctacggcctggacaagctgatcaacaccatcatcgtcgatatcggcgccggaaccacggatatctgcgccctgaagggcactgttcccggccccgaagatcaggttaccctgaccaaggctggcaactatgtggatgagcggctccagaacgccatcttggaacgtcatcccgaacagctgatgaacgtgaatgtcgcctgcgcggtgaaagagcagttctccttcgtcggcacccccaccgaggttgcctcgttcgagttccgcgccgccggcaagcccgtgcgcgctgacgtgaccgagccggtgaagatcgcctgcgaggcgctgatgcccgacattatcgaaagcatcgagacgctgctgcgttcgttccagcccgaatatcaggccaccgtgcttcaaaacatcgtgtttgccggcggcggctcgcgcattcgcggattggcggcctacgtcaaggaaaagctgcgtcccttcggcgatgcgaacgtgacctgcgtcaaggaccccactttcgacggttgccggggcgccttgcgtctggcggaagagcttccgccgcagtattggcgtcagcttggagacgtctccggctcgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z